Abstract: A method is provided for manipulating objects in a cavity including a liquid, the method including providing in at least one region of the cavity objects capable of absorbing light in a given wavelength range, forming an aggregate of the objects by submitting them to an acoustic field, and disrupting the aggregate by submitting the aggregate to a light beam emitting at the given wavelength range. Also provided is a device for manipulating objects.

Abstract: A microfluidic chip, in particular for a cell culture, the chip including a block made from biocompatible material, a passage channel made in the block for the passage of cells bathed in a liquid, in particular a nutrient liquid, a resonant cavity made in the block, connected to the passage channel and including walls for containing the cells originating from the passage channel, a generator generating acoustic waves capable of forming at least one cell aggregate in acoustic levitation in the resonant cavity, and at least one optical emitter capable of illuminating cells in the resonant cavity through at least one wall of the resonant cavity and simultaneous to the generation of acoustic waves in such a way as to structure the at least one aggregate by means of the optical exclusion technique.

Abstract: A method is provided for manipulating objects in a cavity including a liquid, the method including providing in at least one region of the cavity objects capable of absorbing light in a given wavelength range, forming an aggregate of the objects by submitting them to an acoustic field, and disrupting the aggregate by submitting the aggregate to a light beam emitting at the given wavelength range. Also provided is a device for manipulating objects.

Abstract: A method of forming an aggregate of objects in a channel including a liquid, the method including: a) providing objects in at least a region of the channel, and b) forming an aggregate of the objects by submitting them to a modulated pulsed acoustic field, wherein the modulated pulsed acoustic field applied at step b) is modulated in amplitude.

Abstract: A method of forming a multilayer aggregate of objects in a channel including a liquid, the method including: a) providing objects at first and second superposed regions of the channel, b) obtaining first and second aggregates of objects, optionally by applying transverse acoustic waves, preferably stationary waves, within each region to objects, and c) bringing the first and second aggregates into contact to form the multilayer aggregate of objects by submitting the first and second aggregates to: gravity in absence of acoustic waves, or to acoustic waves, optionally stationary waves, inducing displacement of the first and second aggregates toward each other.

Abstract: Systems, methods, and devices are provided for manipulating objects in a liquid using a pulsed acoustic field that is modulated in amplitude. The amplitude modulated pulsed acoustic field may form the objects into a layer or layers. The objects may be colloidal objects having an average size of about 50 nm to about 5 ?m, or a mixture of colloidal and non-colloidal objects.

Abstract: A method of forming an aggregate of objects in a channel including a liquid, said method including: a) providing objects in at least a region of the channel, and b) forming an aggregate of said objects by submitting them to a modulated pulsed acoustic field, wherein the modulated pulsed acoustic field applied at step b) is modulated in amplitude.

Abstract: A method of forming a multilayer aggregate of objects in a channel including a liquid, the method including: a) providing objects at first and second superposed regions of the channel, b) obtaining first and second aggregates of objects, optionally by applying transverse acoustic waves, preferably stationary waves, within each region to objects, and c) bringing the first and second aggregates into contact to form the multilayer aggregate of objects by submitting the first and second aggregates to: gravity in absence of acoustic waves, or to acoustic waves, optionally stationary waves, inducing displacement of the first and second aggregates toward each other.

Abstract: The invention relates to a device (1) intended for handling objects (O) present in a channel (2) within a fluid (F), in particular a liquid. The device comprises: a channel (2) extending along a longitudinal axis (X), said channel (2) having a transverse section with a width (_) measured along a first transverse axis (Y) and a thickness measured along a second transverse axis (Z) perpendicular to the first, said width (_) being greater than or equal to the thickness, and said channel comprising first (3) and second (4) walls along the second transverse axis (Z); and an acoustic wave generator (10) generating acoustic waves in the channel from at least one of the walls (3; 4), said acoustic wave generator (10) operating at a frequency f that is different from a resonant frequency f0 of the channel (2) along the second transverse axis (Z).

Abstract: Systems, methods, and devices are provided for manipulating objects in a liquid using a pulsed acoustic field that is modulated in amplitude. The amplitude modulated pulsed acoustic field may form the objects into a layer or layers. The objects may be colloidal objects having an average size of about 50 nm to about 5 ?m, or a mixture of colloidal and non-colloidal objects.

Abstract: The present invention relates to a fluidic separation device comprising: at least one microchannel (2; 66) extending along a longitudinal axis (X), the microchannel having a cross-section that presents a width measured along a first transverse axis (Y) and a thickness measured along a second transverse axis (Z) perpendicular to the first, the width being greater than the thickness, the microchannel including, along the second transverse axis, bottom and top walls (3 and 4); at least first, second, and third inlets (7, 8, and 9) in fluidic communication with the microchannel (2), the second inlet (8) being disposed on the second transverse axis (Z) between the first and third inlets (7 and 9); and at least first and third transverse separation walls (10 and 11) respectively separating the first and second inlets and the second and third inlets, the first and second separation walls (10; 11) being arranged in such a manner that the second inlet (8) is separated from each of said bottom and top walls (3 and 4

Abstract: The present invention relates to a fluidic separation device comprising: at least one microchannel (2; 66) extending along a longitudinal axis (X), the microchannel having a cross-section that presents a width measured along a first transverse axis (Y) and a thickness measured along a second transverse axis (Z) perpendicular to the first, the width being greater than the thickness, the microchannel including, along the second transverse axis, bottom and top walls (3 and 4); at least first, second, and third inlets (7, 8, and 9) in fluidic communication with the microchannel (2), the second inlet (8) being disposed on the second transverse axis (Z) between the first and third inlets (7 and 9); and at least first and third transverse separation walls (10 and 11) respectively separating the first and second inlets and the second and third inlets, the first and second separation walls (10; 11) being arranged in such a manner that the second inlet (8) is separated from each of said bottom and top walls (3 and