Patents by Inventor May Hui
May Hui has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20080234592Abstract: A method of recording information for use in generating an electrocardiogram indicative of a health status of a user's heart, the method being operable using a device (1) which includes: a housing (2); a processor (3) disposed in the housing (3); first and a second input terminal (4a,4b) disposed on the housing (2), wherein the first and second input terminals (4a,4b) are operatively connected to the processor (3); and a display (5) operatively connected to the processor (3); wherein the method includes the steps of: (i) contacting a right and left thumb (8a,8b) of the user with the first and second input terminals (4a,4b) respectively; and (ii) the processor (3) detecting an ECG signal formed between the first and second input terminals (4a,4b), wherein the ECG signal is indicative of the health status of the user's heart.Type: ApplicationFiled: September 28, 2007Publication date: September 25, 2008Inventors: Meng Huat Damian Lim, Agustin Marjanti, Chin Han Kok, May Hui Shirley Koh, Xin Liu, Kok Beng Neo
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Publication number: 20030082535Abstract: Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide triphosphate feedstocks, and a chain terminating nucleotide triphosphate. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: ApplicationFiled: March 7, 2001Publication date: May 1, 2003Inventors: James Leushner, May Hui, James M. Dunn, Jean-Michel Lacroix
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Patent number: 6413718Abstract: Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The reaction mixture also includes an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing the unconventional nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: GrantFiled: April 24, 1998Date of Patent: July 2, 2002Assignee: Visible Genetics Inc.Inventors: James Leushner, Jean-Michel Lacroix, May Hui, James M. Dunn, Marina T. Larson
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Publication number: 20020037502Abstract: Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The reaction mixture also includes an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing the unconventional nucleotide. The mixture is processed through multiple thermal cycles for annealing extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: ApplicationFiled: April 24, 1998Publication date: March 28, 2002Inventors: JAMES LEUSHNER, JEAN-MICHEL LACROIX, MAY HUI, JAMES M. DUNN, MARINA T. LARSON
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Patent number: 6274315Abstract: Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The reaction mixture also includes an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing the unconventional nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: GrantFiled: April 24, 1998Date of Patent: August 14, 2001Assignee: Visible Genetics Inc.Inventors: James Leushner, Jean-Michel Lacroix, May Hui, James M. Dunn, Marina T. Larson
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Patent number: 6214555Abstract: Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide triphosphate feedstocks, and a chain terminating nucleotide triphosphate. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: GrantFiled: May 13, 1999Date of Patent: April 10, 2001Assignee: Visible Genetics Inc.Inventors: James Leushner, May Hui, James M. Dunn, Jean-Michel LaCroix
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Patent number: 6083699Abstract: A method is provided for simultaneously determining the positions of a selected nucleotide base in a target region of both strands of a denatured duplex nucleic acid polymer. The nucleic acid polymer is combined with a reactant mixture comprising first and second oligonucleotide primers, said primers binding to the sense and antisense strands, respectively, of the nucleic acid polymer at a location flanking the target region; a thermostable DNA polymerase; a chain-terminating nucleotide triphosphate complementary to the selected nucleotide base; and other reagents for synthesis of chain extension products to form a reaction mixture. This mixture is processed through a plurality of thermal cycles, each including at least a chain extension phase and a denaturation phase to produce chain extension products. These chain extension products are evaluated to determine the positions of the selected bases.Type: GrantFiled: January 20, 1998Date of Patent: July 4, 2000Assignee: Visible Genetics Inc.Inventors: James Leushner, May Hui, James M. Dunn, Marina T. Larson, Jean-Michel Lacroix, Robert Shipman
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Patent number: 6063567Abstract: Reliable and cost effective testing for mutations in the RB1 gene can be accomplished by (a) quantitatively amplifying exons of the sample RB1 gene using primers complementary to intron regions flanking each exon; and (b) determining the lengths and/or quantities of the amplification products for each exon and comparing that length or quantity to the length or quantity of amplification products obtained when a wild-type RB1 gene is amplified using the same primers. Differences in length between an amplified sample exon and the corresponding amplified wild-type exon reflect the occurrence on an insertion or deletion mutation in the sample RB1 gene. Differences in quantity reflect the complete absence of an exon, or heterozygosity for a mutant exon. Next, the nucleic acid sequence of each exon found to contain an insertion or deletion mutation is determined, or of all exons in the event no insertion or deletion mutations are identified.Type: GrantFiled: January 7, 1997Date of Patent: May 16, 2000Assignee: Visible Genetics Inc.Inventors: Brenda L. Gallie, James M. Dunn, John K. Stevens, May Hui
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Patent number: 6013436Abstract: The instant invention relates to methods and products for the diagnosis of mutations in the von Hippel-Lindau tumor suppressor gene. More specifically, the products are DNA oligonucleotides for use in amplifying and sequencing genomic DNA and kits including these oligonucleotides. The method of the invention relates to a hierarchical system for cost-effective diagnosis of von Hippel-Lindau tumor suppressor disease-associated mutations.Type: GrantFiled: August 19, 1996Date of Patent: January 11, 2000Assignee: Visible Genetics, Inc.Inventors: May Hui, James M. Dunn, John K. Stevens, Denis Capatos, David E. Matthews
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Patent number: 5888736Abstract: Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenase.TM. which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide triphosphate feedstocks, and a chain terminating nucleotide triphosphate. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: GrantFiled: February 27, 1997Date of Patent: March 30, 1999Assignee: Visible Genetics, Inc.Inventors: Jean-Michel Lacroix, James Leushner, May Hui, James M. Dunn, Marina T. Larson
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Patent number: 5830657Abstract: Sequencing of a selected region of a target nucleic acid polymer in a genomic DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase.TM. which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.Type: GrantFiled: July 19, 1996Date of Patent: November 3, 1998Assignee: Visible Genetics Inc.Inventors: James Leushner, May Hui, James M. Dunn, Marina T. Larson
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Patent number: 5789168Abstract: Amplification and sequencing of a selected region of a target nucleic acid polymer are be performed in a single vessel. The sample is added to an amplification mixture containing a thermally stable polymerase and nucleoside feedstocks. Chain terminating dideoxynucleosides are added either at the beginning of the amplification reaction or during the course of the amplification. A thermally stable polymerase which incorporates dideoxynucleotides into an extending oligonucleotide at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleosides can be used in the amplification mixture or added with the chain terminating nucleoside.Type: GrantFiled: May 1, 1996Date of Patent: August 4, 1998Assignee: Visible Genetics Inc.Inventors: James Leushner, May Hui, James M. Dunn, Marina T. Larson