Patents by Inventor May Tom-Moy
May Tom-Moy has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9234892Abstract: A method for labelling a tissue section is provided. In certain embodiments, the method may comprise: (a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (c) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. A kit for performing the method is also provided.Type: GrantFiled: May 8, 2014Date of Patent: January 12, 2016Assignee: Agilent Technologies, Inc.Inventors: May Tom-Moy, Nazumi Alice Yamada
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Publication number: 20150323532Abstract: A method for labelling a tissue section is provided. In certain embodiments, the method may comprise: (a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (c) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. A kit for performing the method is also provided.Type: ApplicationFiled: May 8, 2014Publication date: November 12, 2015Applicant: Agilent Technologies, Inc.Inventors: May Tom-Moy, Nazumi Alice Yamada
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Publication number: 20090142775Abstract: Methods and kits for separating organelles of a specific type from a mixture of organelles of different types are described. The methods comprise providing a mixture of organelles of different types, adding probes to the mixture to form an organelle-probe complex with a distinct diffusion coefficient, and separating the organelles of the specific type from the mixture of organelles based upon the diffusion coefficient of the complex. The probes used in these methods include an affinity portion which binds selectively to the organelle of the specific type and a tag portion which imparts the distinct diffusion coefficient to the complex.Type: ApplicationFiled: November 30, 2007Publication date: June 4, 2009Applicant: Agilent Technologies, Inc.Inventor: May Tom-Moy
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Patent number: 7488607Abstract: Methods and apparatus for electronically addressing and interrogating microarrays are disclosed. The described microarrays include a plurality of features disposed on a substrate. Each of the features has a first electrode disposed on the substrate, a second electrode disposed on the substrate, and a probe disposed between the first electrode and second electrode. The substrate also includes addressing circuitry in operable relation to the features. Method of using the microarrays are also described.Type: GrantFiled: September 30, 2003Date of Patent: February 10, 2009Assignee: Agilent Technologies, Inc.Inventors: May Tom-Moy, Carl Myerholtz, August Hidalgo, William McAllister
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Publication number: 20070202008Abstract: Systems and methods for nanopore flow cells are provided.Type: ApplicationFiled: February 28, 2006Publication date: August 30, 2007Inventors: Carol Schembri, May Tom-Moy
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Publication number: 20060105461Abstract: Nanopore analysis systems, methods of preparing nanopore analysis systems, and methods of automating the analysis of samples using nanopore analysis systems, are provided.Type: ApplicationFiled: October 22, 2004Publication date: May 18, 2006Inventors: May Tom-Moy, Reid Brennen, Arthur Schleifer, Hongfeng Yin, Tom Goor, James Young, Richard Pittaro
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Publication number: 20050255515Abstract: The invention provides methods and sensors for detecting target biological molecules. Biosensors feature photoactivatable charge separation moieties capable of generating electron-hole pairs upon photoinduction. Photoinduced charge carriers participate in redox reactions that are detectable, for example, by optical, chemical, or electronic means.Type: ApplicationFiled: June 14, 2005Publication date: November 17, 2005Inventors: Daniel Roitman, May Tom-Moy, Seiji Inaoka, Robert Ach
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Publication number: 20050136408Abstract: Methods and systems for characterizing a polymer in a sample are provided. In the subject methods, a sample that includes a polymer labeled with at least one nanoparticle is contacted with a nanopore under conditions so that the polymer translocates through the nanopore. A signal is read from the nanopore to characterize the translocated polymer. The subject systems include a nanopore device and a polymer that is labeled with at least one nanoparticle. Also provided is programming stored on a computer-readable medium for use in practicing the subject methods. Kits for use in practicing the subject methods are also provided.Type: ApplicationFiled: December 19, 2003Publication date: June 23, 2005Inventors: May Tom-Moy, Richard Pittaro, Carl Myerholtz
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Publication number: 20050079505Abstract: Methods and apparatus for electronically addressing and interrogating microarrays are disclosed. The described microarrays include a plurality of features disposed on a substrate. Each of the features has a first electrode disposed on the substrate, a second electrode disposed on the substrate, and a probe disposed between the first electrode and second electrode. The substrate also includes addressing circuitry in operable relation to the features. Method of using the microarrays are also described.Type: ApplicationFiled: September 30, 2003Publication date: April 14, 2005Inventors: May Tom-Moy, Carl Myerholtz, August Hidalgo, William McAllister
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Publication number: 20050069905Abstract: A device for electronic detection of a target includes a probe, e.g. an oligonucleotide, attached to a pad of resistive material, wherein the pad is adjacent a first electrode and also is adjacent a second electrode. In use, the probe is contacted with a sample containing the target, e.g. a target nucleic acid, under conditions and for a time sufficient to allow target to bind the probe. An enhancement reaction is then applied to result in a change in an observable property of the device. The observable property is then monitored using measurement apparatus operably associated with the device. Typically, multiple devices will be present on an array of devices, allowing multiplex analysis of multiple different targets using a single array of devices.Type: ApplicationFiled: September 30, 2003Publication date: March 31, 2005Inventors: Carl Myerholtz, May Tom-Moy, August Hidalgo, William McAllister
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Patent number: 6723516Abstract: A method is provided for continuously monitoring for the presence or quantity of an analyte in a flowing liquid stream. The method involves binding an analyte-specific receptor species to the surface of a piezoelectric substrate, contacting the surface bound receptor species with the flowing liquid stream and quantitating the presence of the analyte. A novel apparatus for detecting the presence of an analyte in a liquid chromatography eluant is provided as well.Type: GrantFiled: October 24, 1996Date of Patent: April 20, 2004Assignee: Agilent Technologies, Inc.Inventors: May Tom-Moy, Thomas P. Doherty, Richard L. Baer, Darlene J. Spira-Solomon
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Publication number: 20030186245Abstract: The invention provides methods and sensors for detecting target biological molecules. Biosensors feature photoactivatable charge separation moieties capable of generating electron-hole pairs upon photoinduction. Photoinduced charge carriers participate in redox reactions that are detectable, for example, by optical, chemical, or electronic means.Type: ApplicationFiled: March 28, 2002Publication date: October 2, 2003Inventors: Daniel B. Roitman, May Tom-Moy, Seiji Inaoka, Robert A. Ach
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Patent number: 6586232Abstract: A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions.Type: GrantFiled: March 21, 2001Date of Patent: July 1, 2003Assignee: Agilent Technologies, Inc.Inventors: May Tom-Moy, Carl Alan Myerholtz
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Publication number: 20020192718Abstract: A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions.Type: ApplicationFiled: March 21, 2001Publication date: December 19, 2002Inventors: May Tom-Moy, Carl Alan Myerholtz
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Patent number: 6235488Abstract: A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions.Type: GrantFiled: April 29, 1992Date of Patent: May 22, 2001Assignee: Agilent Technologies, Inc.Inventors: May Tom-Moy, Carl Alan Myerholtz
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Patent number: 6077674Abstract: A method of making full-length oligonucleotide arrays provides for the purification of pre-synthesized full-length oligonucleotides from shorter length oligonucleotides and other impurities at the same time the oligonucleotides are deposited on the array. A synthesized mixture that includes desired full-length oligonucleotides and some capped shorter length or "failed" oligonucleotide sequences, is reacted with a linking agent to add a linking group on to the free-end of the full-length oligonucleotides but not the shorter-length oligonucleotides. The resulting mixture is deposited on an array without first separately purifying the mixture to remove the unwanted shorter-length oligonucleotides. After deposition, unbound material, including the shorter length oligonucleotide sequences and other impurities, is removed.Type: GrantFiled: October 27, 1999Date of Patent: June 20, 2000Assignee: Agilent Technologies Inc.Inventors: Arthur Schleifer, May Tom-Moy
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Patent number: 5527711Abstract: A method is provided for detecting an analyte of interest in a sample. The method involves binding the analyte to the surface of a substrate through a biotin-biotin binding protein interaction, contacting the surface-bound analyte with a quantitatively detectable analyte-binding moiety that binds thereto, measuring the quantity of detectable moiety bound to the substrate surface and deriving therefrom the quantity of analyte in solution. A preferred use for the present method is in conjunction with a piezoelectric surface transverse wave device. Novel reagents useful for carrying out the inventive method are provided as well.Type: GrantFiled: December 13, 1993Date of Patent: June 18, 1996Assignee: Hewlett Packard CompanyInventors: May Tom-Moy, Joel Myerson, Karla M. Robotti
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Patent number: 5306644Abstract: A system for accurate and precise measurements of analyte(s) in a system. The measurement system comprises piezoelectric surface wave sample devices, at least one piezoelectric surface wave reference device, and the measurement instrument.Type: GrantFiled: April 1, 1993Date of Patent: April 26, 1994Assignee: Hewlett-Packard CompanyInventors: Carl A. Myerholtz, May Tom-Moy, Darlene J. Spira-Solomon, Richard L. Baer, Thomas P. Doherty
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Patent number: 5229301Abstract: A mass biosensor method provides enhanced quantification of analyte concentrations in a sample. In a direct approach, an analyte is derivatized to form an analyte chelate and then specifically bound to a sensor. In an indirect approach, a complement of the analyte is derivatized to form a complement chelate which is then bound to a sensor. In a direct/indirect hybrid approach, an analog of the analyte is derivatized to form an analog chelate that is bound to a sensor in competition with the sample analyte. In all three approaches, mass measurements taken as the ligand chelate attaches to the sensor permit the concentration of the analyte in the sample to be calculated. Once measurement is completed, a dissociation treatment is applied to dissociate the derivatized species from the sensor so that the sensor can be reused. The effects of the dissociation treatment can be monitored using phosphorescence detection.Type: GrantFiled: June 7, 1991Date of Patent: July 20, 1993Assignee: Hewlett-Packard CompanyInventors: Darlene J. Spira-Solomon, May Tom-Moy, Carl A. Myerholtz
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Patent number: 5130257Abstract: A sensor (11, 12, 13, 15) suitable for use as a viscosity sensor, a chemically selective sensor, or a chemically specific sensor. The sensor (11, 12, 13, 15) is a surface transverse wave (STW) device that, for solute concentration measurements, includes a binding layer (18) selected to bind to the solute to be measured. This binding layer (18) can be an antibody so that the sensor detects a particular antigen.Type: GrantFiled: September 29, 1988Date of Patent: July 14, 1992Assignee: Hewlett-Packard CompanyInventors: Richard L. Baer, Carl Myerholtz, Curt Flory, May Tom-Moy