Abstract: The disclosure provides amino acid sequence variants of Bacillus thuringiensis (Bt) toxins and methods of producing the same. Some aspects of this disclosure provide methods for generating Bt toxin variants by continuous directed evolution. Some aspects of this disclosure provide compositions and methods for pest control using the disclosed variant Bt toxins.

Abstract: The disclosure provides amino acid sequence variants of Bacillus thuringiensis (Bt) toxins and methods of producing the same. Some aspects of this disclosure provide methods for generating Bt toxin variants by continuous directed evolution. Some aspects of this disclosure provide compositions and methods for pest control using the disclosed variant Bt toxins.

Abstract: A microfluidic platform for single-cell capture, stimulation, and imaging. It passively traps 4,000 single cells on a 4.5 mm2 footprint in 30 seconds, with a single-cell loading efficiency of 95%. The array format and optimized geometry allows for easy, robust and efficient single-cell loading, while maintaining captured cells in a low shear stress environment for long-term studies. Because cells are captured sequentially, the system is adequate for rare cell samples. Trapped cells can be exposed to various environmental conditions and chemical stimulus and their dynamic response can be monitored over time. The information gained from high-throughput, single-cell time lapsed imaging presents new opportunities in quantifying cellular responses, as averaged information by other measurement methods eliminates sub-population phenotypes.

Abstract: The disclosure provides amino acid sequence variants of Bacillus thuringiensis (Bt) toxins and methods of producing the same. Some aspects of this disclosure provide methods for generating Bt toxin variants by continuous directed evolution. Some aspects of this disclosure provide compositions and methods for pest control using the disclosed variant Bt toxins.

Abstract: The disclosure provides amino acid sequence variants of Bacillus thuringiensis (Bt) toxins and methods of producing the same. Some aspects of this disclosure provide methods for generating Bt toxin variants by continuous directed evolution. Some aspects of this disclosure provide compositions and methods for pest control using the disclosed variant Bt toxins.

Abstract: Aspects of the present disclosure generally relate to methods for analyzing spatial trajectories of cells by identifying metrics corresponding to cell spatial properties and analyzing the metrics over time.

Abstract: A system and method that provides intra-day confirmation of non-anonymous trade transactions made at an exchange includes the entry into a computer system of first data relative to the trade transaction on behalf of one party to the trade transaction, wherein the first data include a code identifying an opposing party to the transaction. A message is sent to the designated opposing party prior to submitting the trade transaction for matching prior to end-of-day clearing. The trade transaction is matched to second data relative to the trade transaction entered into the system based on identity between certain elements of the first data and the second data, and a confirmation is sent to at least one of the parties to the trade transaction.

Abstract: A system and method that provides a message engine to facilitate intra-day receiving of first data relative to the trade transaction on behalf of one party to the trade transaction, wherein the first data include a code identifying an opposing party to the transaction; sending a message to the designated opposing party prior to submitting the trade transaction for matching prior to end-of-day clearing; and monitoring a message queue for a response and sending a message to at least one of the parties based on the response.