Abstract: The present invention relates to a self-contained assay device which is capable of detecting various analyte(s), including bioanalytes, in specimens for example, from biological sources. The assay device includes a first housing and a specimen holder rotatably fit in the first housing. The specimen holder has a center portion, a circular flange surrounding the center portion and a pin member extending from the underneath of the center portion. The center portion has a radial slot extending from its peripheral end toward a closed end. A spring/latch assembly is adapted to be held in the slot on the specimen holder and includes a spring member disposed in the slot near its closed end, a latch member having a remote end and a plurality of plunger members. The assay device also includes a second housing, preferably a cam-plate fixedly fit in the first housing.

Abstract: The assay device includes a first housing and a specimen holder rotatably fit in the first housing. The specimen holder has a center portion, a circular flange surrounding the center portion and a pin member extending from the underneath of the center portion. The center portion has a radial slot extending from its peripheral toward its center. A spring/latch assembly is adapted to be held in the slot on the specimen holder and includes a spring member disposed near the center of the center portion and a latch member having a remote end. The assay device also includes a second housing, preferably a cam plate fixedly fit in the first housing. The cam plate has a rim portion surrounding a concave portion adapted to accommodate the center portion of the specimen holder and an opening on the rim portion for adding a specimen to be tested. A plurality of cam-shaped chambers are provided in the rim portion and communicate with the concave portion.