Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g.

Abstract: A system of and method for installation and use thereof that provides for application of karaoke system components applicable mobile environments, including integrated or retrofittable components in a manner that allows vehicle occupants to selectively utilize in combination a karaoke system together with audio and related operating systems to provide an integrated karaoke system.

Abstract: A system of and method for installation and use thereof that provides for application of karaoke system components applicable either for desktop or mobile environments, including integrated or retrofittable components in a manner that allows desktop users/vehicle occupants to selectively utilize in combination a karaoke system together with audio and related operating systems to provide an integrated karaoke system.