Abstract: Novel tools and techniques are provided for implementing real-time fault management or real-time fault management system (“RFM”). In various embodiments, RFM may receive alerts from or associated with network devices (e.g., layer 2, 3, and/or 4 devices, or the like) that are disposed in a plurality of disparate networks that may utilize different alert management protocols and/or different fault management protocols. RFM may collect, enrich, normalize, aggregate, and display the alerts in a user interface to facilitate addressing of the alerts by a user. To enable continuous and real-time functionality, RFM may be implemented in a plurality of siloed platforms in a primary data center, with processing of alerts being load balanced across the siloed platforms, with mirrored group of siloed platforms in a secondary data center located geographically distant from the primary data center and configured to be on “hot standby” and to completely take over RFM processing operations.

Abstract: Methods for amplifying the Raman signal of primary SERS nanoparticles are provided. One method generally includes binding secondary SERS particles to the primary SERS nanoparticles after binding of the primary SERS nanoparticles. In another method, secondary SERS nanoparticles are brought in close proximity to the primary SERS nanoparticles, wherein the secondary nanoparticles are free of a reporter molecule or have a reporter molecule different from that of the primary SERS nanoparticles.

Abstract: Methods for amplifying the Raman signal of primary SERS nanoparticles are provided. One method generally includes binding secondary SERS particles to the primary SERS nanoparticles after binding of the primary SERS nanoparticles. In another method, secondary SERS nanoparticles are brought in close proximity to the primary SERS nanoparticles, wherein the secondary nanoparticles are free of a reporter molecule or have a reporter molecule different from that of the primary SERS nanoparticles.

Abstract: A coated particle, as well as a method of making a coated particle are described. The coated particle includes a core and a coating. The coating at least partially covers the core. The method of making a coated particle includes i) providing a colloidal solution having a core; ii) providing a coating precursor to the colloidal solution to form a resulting solution; and iii) providing an acid, and or adjusting a pH of the resulting solution, and or concentrating.

Abstract: A coated particle, as well as a method of making a coated particle are described. The coated particle includes a core and a coating. The coating at least partially covers the core. The method of making a coated particle includes i) providing a colloidal solution having a core; ii) providing a coating precursor to the colloidal solution to form a resulting solution; and iii) providing an acid, and or adjusting a pH of the resulting solution, and or concentrating.

Abstract: A lateral flow device is disclosed. The lateral flow device includes a substrate having a flow path and a detection zone disposed along the flow path. The detection zone includes an immobilized target-binding moiety directed against a target of a Raman-active complex. Also disclosed is a method of conducting a lateral flow assay and detection system. The method includes i) defining a flow path having a detection zone; ii) flowing a sample down the flow path; and iii) immobilizing a Raman-active complex if present, at the detection zone. The sample includes a Raman-active complex or a Raman-active tag.

Abstract: A super-paramagnetic Raman-active complex that includes a Raman-active tag attached to a target and a super-paramagnetic bead attached to the target is disclosed. A method of separating a Raman or surface enhanced Raman-active tag unattached to a target from a Raman-active complex is also disclosed. The Raman-active complex includes a Raman-active tag attached to a target. The method includes providing a mixture that includes at least one Raman-active tag unattached to a target and at least one super-paramagnetic Raman-active complex; and applying a magnetic field to the mixture.

Abstract: A transgenic plant is prepared by a method in which a plant cell is transformed with a chimaeric gene comprising a promoter and a gene encoding a polypeptide which displays the activity of an enzyme which regulates the amount of a metabolic intermediate in glycolysis or in a pathway for the synthesis or degradation of starch, sucrose or reducing sugar from a glycotyltic intermediate. In stored potatoes the subject of the invention an increased level of phosphofructokinase results in reduced accumulations of sugars in the tubers.

Abstract: A transgenic plant is prepared by a method n which a plant cell is transformed with a chimaeric gene comprising a promoter and a gene encoding a polypeptide which displays the activity of an enzyme which regulates the amount of a metabolic intermediate in glycolysis or in a pathway for the synthesis or degradation of starch, sucrose or reducing sugar from a glycotyltic intermediate. In stored potatoes the subject of the invention an increased level of phosphofructokinase results in reduced accumulations of sugars in the tubers.