Abstract: A device for washing a suspension of cells. The device includes a bowl-shaped basin having a top end and a bottom end and an axis extending from the top end to the bottom end, and an inlet port formed in the basin. The inlet port is positioned at an angle to allow for a wash solution to be injected or delivered into the basin and circulate around the basin about the central axis. A suspension of cells can be washed in the circulating wash solution. The circulating wash solution forces the cells to settle at the bottom of the basin, where they can be extracted.

Abstract: A separation device that can include a separation tube or container that has a wall defining an internal volume into which a material can be placed is disclosed. The material can include a multi-component mixture or solution. A secondary tube or withdrawal cannula can be placed and/or moved within the tube to withdrawal a material form the tube.

Abstract: Oxygen diffusive wound dressings and methods of manufacturing and use are described herein. The wound dressing may generally provide a ready supply of oxygen to a wound being treated via one or more oxygen conduits which are designed to pass oxygen from ambient air or other oxygen reservoirs into proximity to the wound, and may also provide for exudate removal through transecting channels in fluid communication with both the wound surface and a hydrophilic absorbent material.

Abstract: A separator that uses centrifugation to fractionate a suspension such as blood comprises a separation container and a buoy. The buoy is carried in the separation container and has a tuned density that is configured to reach an equilibrium position in a suspension. The guide surface is carried on the buoy upper surface and is inclined to an accumulation position near a buoy perimeter. The buoy suspension fractionation system can be used in a method of isolating a fraction from a suspension, and in a method for re-suspending particulates for withdrawal.

Abstract: Extracting or removing at least a portion of liquid phase from a whole sample using a centrifugal force is disclosed. Centrifugal forces are used to apply pressure to a whole sample and drive a liquid phase through a passage region that can be perforated and/or porous and maintain a drier portion within a separation container. The whole sample can be dried, which includes a remaining sample where excess or a selected amount of liquid is removed. Direct access to the separation container or area can then be made to provide for an efficient withdrawal of the drier material from the separation container.

Abstract: Oxygen diffusive wound dressings and methods of manufacturing and use are described herein. The wound dressing may generally provide a ready supply of oxygen to a wound being treated via one or more oxygen conduits which are designed to pass oxygen from ambient air or other oxygen reservoirs into proximity to the wound, and may also provide for exudate removal through transecting channels in fluid communication with both the wound surface and a hydrophilic absorbent material.

Abstract: Apparatus and methods for generating a solution rich in interleukin-1 receptor antagonist from a tissue comprising cytokine-producing cells. The apparatus can include a filter used with a separation system. The separation system can include a float.

Abstract: Separation of a wash liquid from red blood cells is disclosed using a filter separation and pressure differential system. The filter may include a membrane filter.

Abstract: A separation system for separating a multiple component material into at least two fractions. The separation system includes a separation device having a first end, a second end opposite to the first end, and a sidewall that extends between the first end and the second end to define a separation chamber having an interior volume. The system also includes a valve moveable between an open position and a closed position, the valve is mounted at a fixed location within the separation chamber at a position that is closer to the second end than to the first end and is spaced apart from the second end, the valve is operable to isolate a first fraction of the multiple component material having a first density on a first side of the valve from a second fraction having a second density on a second side of the valve that is opposite to the first side.

Abstract: A method for washing a suspension of cells including a suspension fluid and a plurality of cells is provided. The method includes using centripetal force to generate a layer of wash solution against a cylindrical wall and to generate a cell suspension layer adjacent to the layer of wash solution. The method also includes forcing the cells through the layer of wash solution to generate a layer of clean cells. Devices for performing the method are also provided.

Abstract: Disclosed is a device for separating a cellular component from a multicomponent fluid. The device can include a body, a first acoustic wave generator, and a second acoustic wave propagating component. The body can define a channel having a first surface, an opposing second surface, a first side, and an opposing second side. The channel can extend along a longitudinal axis from a first end to an opposing second end. The first acoustic wave generator can be coupled to the first surface. The second acoustic wave propagating component can be coupled to the second surface. The first acoustic wave generator and second acoustic wave propagating component can be configured to generate a bulk standing acoustic wave in the channel.

Abstract: Devices for washing a composition including cells are provided. The devices include a separation channel, a first wave component and a second wave component. The separation channel is positioned between the first and second wave components. Collectively, the wave components generate a standing bulk acoustic wave that isolates cells from the composition.

Abstract: Devices and methods for washing a suspension of cells are provided. The devices include concentric center, intermediate, and outer compartments. The center compartment and the intermediate compartment are in communication with each other by a first valve and the intermediate and outer compartments are in communication with each other by an opening, aperture, or second valve. The methods include loading a suspension of cells into the center compartment, loading a wash solution into the intermediate compartment, and rotating the device. Centripetal force forces cells through the wash solution and into the outer compartment, where they are isolated and collected.

Abstract: Separation devices for separating a component from a multicomponent mixture are provided. The separation devices can include a body that defines a separation channel with a cross-sectional geometry that is not circular, rectangular, or square. The separation devices can be positioned relative to a base having an acoustic wave generator. A standing bulk acoustic wave generated by the acoustic wave generator can separate one or more components from the multicomponent mixture. Methods are provided for separating one or more components from a multicomponent mixture using the separation devices.

Abstract: Methods for treating a spinal disorder, such as spinal pain or spinal inflammation, using a blood-derived composition having two or more of IL1-ra, sTNF-R1, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-?1, and sIL-1RII. Compositions may also contain white blood cells and platelets.

Abstract: An apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at various fractions in the apparatus. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient. Also selected fractions from the sample can be applied to a patient, either alone or as part of a mixture.

Abstract: A material separation system and method is disclosed. The system may include a buoy having a volume that includes or houses a reacting component. The buoy may be placed in a container to hold the material to be separated during a separation procedure. The separated material may be used to various procedures following separation.

Abstract: A device for washing a suspension of cells. The device includes a bowl-shaped basin having a top end and a bottom end and an axis extending from the top end to the bottom end, and an inlet port formed in the basin. The inlet port is positioned at an angle to allow for a wash solution to be injected or delivered into the basin and circulate around the basin about the central axis. A suspension of cells can be washed in the circulating wash solution. The circulating wash solution forces the cells to settle at the bottom of the basin, where they can be extracted.

Abstract: A separator system operable to use centrifugation to fractionate a multiple component material, such as a suspension including blood, comprises a buoy. The buoy can be carried in a separation container and has a tuned density that is configured to reach an equilibrium position in the multiple component material. A guide surface is carried on a buoy upper surface and is inclined to an accumulation position near a buoy perimeter. The buoy suspension fractionation system can be used in a method of isolating a fraction from a suspension, and in a method for re-suspending particulates for withdrawal.

Abstract: Disclosed is an apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at various fractions in the apparatus. A buoy system that may include a first buoy portion and a second member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient. Also selected fractions from the sample can be applied to a patient, either alone or as part of a mixture.