Abstract: Digital microfluidic devices, and methods for the control and fabrication thereof, and provided in which an integrated nanostructured electrodeposited electrode is provided such that the digital microfluidic array can be actuated to contact a droplet with the nanostructured electrodeposited electrode. In some embodiments, digital microfluidic devices are provided having an integrated electrochemical sensor, where the working electrode is provided in the form of a nanostructured electrodeposited electrode. Various methods of fabricating such integrated device are described, including methods that employ a lift-off process that exposes an underlying base electrode for the electrodeposition of a nanostructured electrodeposited electrode, while providing a hydrophobic surface for droplet transport.