Abstract: Methods for generating CD31+CD34+CD143+CD309+GATA2+FLI1+RUNX1+vWF+hemogenic endothelial cells (HECs) are provided using chemically-defined culture media that allow for generating HECs from early mesoderm progenitors in as little as three days and from pluripotent stem cells in as little as five days. Culture media, isolated cell populations and kits are also provided.

Abstract: A method of constructing a means suitable for directing or holding water is described. A depression suitable to direct, transport, or hold water, such as a water catchment or irrigation ditch is covered with cloth, any seams are fastened together, then the cloth is coated with a liquid silicone elastomeric composition curable at atmospheric conditions. The coated cloth edges are stabilized. The method yields a depression having a surface impervious to liquid water that has a long expected life due to its resistance to damaging effects such as weathering and exposure to wandering animals.

Abstract: A method of constructing a weatherproof coating over a roof comprises laying a piece or pieces of cloth over a roof, bonding the pieces of cloth together at any seams, adhering the cloth to the roof surface at least at the edges and projections, then coating the cloth with a liquid elastomeric silicone composition. The composition cures under ambient conditions to yield a water impermeable membrane that has been manufactured in place. A preferred embodiment uses unimpregnated, non-woven thermoplastic cloth having an upper surface thermally treated to remove projecting fibers, the thermally treated surface being subsequently wash coated with a layer of liquid elastomeric silicone emulsion except for any surface of the cloth edges which will be subsequently bonded at any seams, the wash coating being cured before application of the coated cloth to the roof.

Abstract: Aquatic plant growth can be reduced or eliminated in a pond or lake by placing a silicone rubber-coated fabric over the area where such plants want to be eliminated.

Abstract: A silicone elastomeric emulsion having improved shelf life is produced by a method which mixes the ingredients of the emulsion with the exception of a filler other than colloidal silica, then ages the mixture for at least two weeks at room temperature then adds the filler other than colloidal silica. The improved emulsion yields a film upon drying that has better physical properties. The emulsion contains an anionically stabilized, hydroxyl endblocked polydiorganosiloxane, colloidal silica, and an alkyl tin salt, as well as the filler other than colloidal silica. The emulsion has a pH of greater than 9 and a solids content of greater than 40 percent by weight. The emulsion is useful in forming a protective elastomeric coating over a substrate and as a caulking material.

Abstract: A method of constructing a means suitable for directing, transporting or holding water is disclosed. Cloth is layed upon a surface convenient to a depression suitable to direct, transport, or hold water. The cloth is coated with a liquid silicone elastomeric composition curable at atmospheric conditions. The composition cures to an elastomer having a modulus which provides sufficient flexibility to the coated cloth so that the coated cloth will assume the contour of the depression, the coated cloth being impervious to liquid water. After curing, the coated cloth is moved to the depression and placed into the depression to cover the surface of the depression. The coated cloth is bonded at seams and the perimeter of the coated cloth is stabilized. The method yields a structure that is economical to manufacture, yet has a long, useful life in such applications as catchments, irrigation ditches, and holding ponds.