Abstract: A method and system for monitoring a user's intoxication including receiving a set of signals, derived from a set of samples collected from the user at a set of time points; providing a sobriety task to the user proximal to a time point of the set of time points; generating a performance dataset characterizing performance of the sobriety task by the user; receiving a supplementary dataset characterizing a demographic profile of the user and/or a physiological state of the user; determining a set of values of an intoxication metric, derived from the set of signals; generating a predicted temporal profile of the intoxication metric for the user based upon the set of values, the set of time points, and the supplementary dataset; generating an analysis of the user's sobriety based upon the performance dataset and the predicted temporal profile; and providing a notification to the user based upon the analysis.

Abstract: The present invention relates to a process for purification a protein comprising a semi-continuous chromatography step whereby the flow-through is collected and re-loaded onto the chromatography matrix.

Abstract: There is disclosed a composition comprising a first construct, wherein the first construct comprises a charge component, a lock component and a key component; wherein the lock component and the key component are specific affinity binding partners and are separated such that they are unable to form a binding pair within the same construct; whereby in a first environmental condition, the charge component imparts a first overall charge to the first construct, the lock component binds to its binding partner on a second construct, and the key component binds to its binding partner on a third construct; and whereby in a second environmental condition, the charge component imparts a second overall charge to the first construct, and the lock and key components are in an unbound state. Also disclosed is a method of purification of a target compound involving the composition.

Abstract: A person support apparatus includes a base, a controller, and a barrier having at least one electrical device. The base includes mounting structures for releasably mounting the barrier to the base. A first electrical connector is in communication with the at least one electrical device and mounted to the barrier. A second electrical connector is in communication with the controller and mounted at one of the mounting structures for connection with the first electrical connector when the barrier is mounted to the base at the mounting structures.

Abstract: The present invention is in the field of purification and protein purification in particular. The invention provides improved techniques for the industrial-scale purification of proteins and other biomolecules. More specifically, it relates to a process for the purification of a compound of interest, such as a protein, preferably an antibody or an antibody fragment using a chromatography step, preferably a semi-continuous chromatography step.

Abstract: The present invention contemplates allele-specific gene editing based on targeting a heterozygous single nucleotide polymorphism (SNP) in a protein coding sequence associated with a genetic disease. The data shown herein demonstrates that the outcome of such gene editing creates a nonesense mutation that results in a marked and selective reduction of mutant protein without affecting wild type protein expression. Expression of a single CRISPR-Cas9 nuclease in neurons generated a high frequency of mutations in the targeted HD allele that included both small insertion/deletion mutations and viral vector insertions. Thus, as disclosed herein, allele-specific targeting of InDel and insertion mutations to heterozygous coding SNPs provides a feasible approach to inactivate autosomal dominant mutations that cause genetic disease.

Abstract: A portable configuration tool for configuring a headwall unit that is adapted to be electrically coupled to an outlet on a headwall communicates with a room device positioned within a common room with the headwall unit. The tool includes a housing, a transceiver for communicating with a portable computer, IR and RF transceivers for communicating with the headwall unit, and a controller adapted to receive a command from the portable computer that instructs the controller to transmit an initial message to the headwall unit using the infrared transceiver, receive an identifier from the headwall unit via the infrared transceiver that uniquely identifies the headwall unit, establish a communication link between the RF transceiver and the headwall unit, receive a configuration setting from the portable computer via the transceiver, and transmit the configuration setting to the headwall unit via at least one of the infrared or RF transceivers.

Abstract: A person support apparatus includes a base, a controller, and a barrier having at least one electrical device. The base includes mounting structures for releasably mounting the barrier to the base. A first electrical connector is in communication with the at least one electrical device and mounted to the barrier. A second electrical connector is in communication with the controller and mounted at one of the mounting structures for connection with the first electrical connector when the barrier is mounted to the base at the mounting structures.

Abstract: Chemically modified crRNAs and tracrRNAs are provided. crRNAs and tracrRNAs with 5? and/or 3? conjugated moieties are provided. crRNAs and tracrRNAs with modifications in the repeat region of the crRNA or the anti-repeat region of the tracrRNA are provided. Methods of using the crRNAs and tracrRNAs for genome editing with a CRISPR nuclease and kits for performing the same are also provided.

Abstract: The present invention relates to a process for purification a protein comprising a semi-continuous chromatography step whereby the flow-through is collected and re-loaded onto the chromatography matrix.

Abstract: A person support apparatus includes a base, a controller, and a barrier having at least one electrical device. The base includes mounting structures for releasably mounting the barrier to the base. A first electrical connector is in communication with the at least one electrical device and mounted to the barrier. A second electrical connector is in communication with the controller and mounted at one of the mounting structures for connection with the first electrical connector when the barrier is mounted to the base at the mounting structures.

Abstract: An optical system for panoramic stereoscopic imaging can include an outer reflector and an inner reflector, which can both be configured to reflect light to a camera. The outer reflector can include striations or other reflection elements to turn the light that is reflected to the camera such that first and second light rays that are parallel and offset from each other (e.g., suitable for stereoscopic 3D viewing) can be reflected by the respective outer and inner reflectors to the camera. The outer reflector can be partially reflective and partially transmissive so that some light can pass through the outer reflector to be reflected by the inner reflector to the camera. The camera can capture a single image having a first portion that corresponds to a view generated by the inner reflector, and a second portion that corresponds to a stereoscopically offset view generated by the outer reflector.

Abstract: A person support apparatus includes a base, a controller, and a barrier having at least one electrical device. The base includes mounting structures for releasably mounting the barrier to the base. A first electrical connector is in communication with the at least one electrical device and mounted to the barrier. A second electrical connector is in communication with the controller and mounted at one of the mounting structures for connection with the first electrical connector when the barrier is mounted to the base at the mounting structures.

Abstract: A method for firmware debug trace capture includes creating a hand-off block (“HOB”), capturing first debug trace statements during a boot sequence of a computer and writing the first debug trace statements to the HOB. A trace memory buffer can be created and the first debug trace statements can be copied from the HOB to the trace memory buffer. Second debug trace statements are captured during the boot sequence and appended to the trace memory buffer. In some configurations, the first debug trace statements can be written to the HOB during the pre-Extensible Firmware Interface initialization (“PEI”) phase of the boot sequence and the second debug trace statements can be written to the trace memory buffer during the driver execution (“DXE”) phase of the boot sequence.

Abstract: The present invention is in the field of purification and protein purification in particular. The invention provides improved techniques for the industrial-scale purification of proteins and other biomolecules. More specifically, it relates to a process for the purification of a compound of interest, such as a protein, preferably an antibody or an antibody fragment using a chromatography step, preferably a semi-continuous chromatography step.

Abstract: A person support apparatus includes a base, a controller, and a barrier having at least one electrical device. The base includes mounting structures for releasably mounting the barrier to the base. A first electrical connector is in communication with the at least one electrical device and mounted to the barrier. A second electrical connector is in communication with the controller and mounted at one of the mounting structures for connection with the first electrical connector when the barrier is mounted to the base at the mounting structures.

Abstract: Technologies are disclosed herein for configuring a system memory of a computer system by determining which, of a plurality, of serial presence detect (SPD) tables to utilize, based at least in part on settings of one or more GPIO pins. The computer system may be available with variations of the configuration of system memory. The SPD tables corresponding to each of the different configurations of the system memory may be stored on the computer system, such as on an NVRAM of the computer system. After identifying the appropriate SPD table, a processor may load the SPD table to a memory reference code of the computer system. In some cases, the state GPIO pins indicating the system memory configuration is set at the time of manufacture of the computer system.

Abstract: A method for firmware debug trace capture includes creating a hand-off block (“HOB”), capturing first debug trace statements during a boot sequence of a computer and writing the first debug trace statements to the HOB. A trace memory buffer can be created and the first debug trace statements can be copied from the HOB to the trace memory buffer. Second debug trace statements are captured during the boot sequence and appended to the trace memory buffer. In some configurations, the first debug trace statements can be written to the HOB during the pre-Extensible Firmware Interface initialization (“PEI”) phase of the boot sequence and the second debug trace statements can be written to the trace memory buffer during the driver execution (“DXE”) phase of the boot sequence.

Abstract: The present invention relates to a process for purification a protein comprising a semi-continuous chromatography step whereby the flow-through is collected and re-loaded onto the chromatography matrix.

Abstract: While traditional wind chimes typically produce pleasing sounds, the notes are random and at the mercy of which way the wind blows. The Captive Wind Chime overcomes this by keeping the chimes stationary and instead using a striker attached to a spinning rod to play notes in order. With the addition of one or more other “layers” of chimes and strikers, it can also produce polyphonic chords. Because of the pivoting blades and clutch the speed of the notes are partially regulated, allowing in its simplest form the rendition of traditional public domain melodies such as “God Bless America” or “How Great Thou Art”. In its most complex form it can produce synchronized polyphonic chord shape in whatever scale desired.