Patents by Inventor Michael Haugwitz

Michael Haugwitz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230235278
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Application
    Filed: March 31, 2023
    Publication date: July 27, 2023
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
  • Patent number: 11643635
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Grant
    Filed: August 21, 2020
    Date of Patent: May 9, 2023
    Assignee: Takara Bio USA, Inc.
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
  • Publication number: 20200377852
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Application
    Filed: August 21, 2020
    Publication date: December 3, 2020
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
  • Patent number: 10793828
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: October 6, 2020
    Assignee: Takara Bio USA, Inc.
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
  • Publication number: 20200232005
    Abstract: Methods of producing single-stranded deoxyribonucleic acids (ssDNAs) are provided. Aspects of the methods include generating a double stranded deoxyribonucleic acid (dsDNA) and then selectively degrading one strand of the dsDNA to produce a ssDNA. ssDNAs produced using methods of the invention find use in a variety of applications, including genomic modification applications. Also provided are compositions, e.g., kits, that find use in practicing various embodiments of the invention.
    Type: Application
    Filed: January 17, 2020
    Publication date: July 23, 2020
    Inventors: Hiroyuki Matsumoto, Michael Haugwitz, Andrew Farmer, Magnolia Bostick
  • Patent number: 10584363
    Abstract: Methods of producing single-stranded deoxyribonucleic acids (ssDNAs) are provided. Aspects of the methods include generating a double stranded deoxyribonucleic acid (dsDNA) and then selectively degrading one strand of the dsDNA to produce a ssDNA. ssDNAs produced using methods of the invention find use in a variety of applications, including genomic modification applications. Also provided are compositions, e.g., kits, that find use in practicing various embodiments of the invention.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: March 10, 2020
    Assignee: Takara Bio USA, Inc.
    Inventors: Hiroyuki Matsumoto, Michael Haugwitz, Andrew Farmer, Magnolia Bostick
  • Publication number: 20190218544
    Abstract: Provided are methods of identifying a cell that has been edited with a gene editing reagent. Also provided are methods of selecting a clone of a cell that has been identified as an edited cell containing a desired edit, as well as methods of gene editing that include such selecting of an edited clone. Methods of assessing the efficacy of gene editing reagents and methods employing a gene editing reagent efficacy assessment are also provided. The instant disclosure further provides kits for use in practicing the subject methods.
    Type: Application
    Filed: October 25, 2018
    Publication date: July 18, 2019
    Inventors: Montserrat Morell Fernández, Patrick Martin, Michael Haugwitz, Andrew Alan Farmer, Baz Smith
  • Publication number: 20170349927
    Abstract: Methods of producing single-stranded deoxyribonucleic acids (ssDNAs) are provided. Aspects of the methods include generating a double stranded deoxyribonucleic acid (dsDNA) and then selectively degrading one strand of the dsDNA to produce a ssDNA. ssDNAs produced using methods of the invention find use in a variety of applications, including genomic modification applications. Also provided are compositions, e.g., kits, that find use in practicing various embodiments of the invention.
    Type: Application
    Filed: June 2, 2017
    Publication date: December 7, 2017
    Inventors: Hiroyuki Matsumoto, Michael Haugwitz, Andrew Farmer, Magnolia Bostick
  • Publication number: 20170130197
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Application
    Filed: January 25, 2017
    Publication date: May 11, 2017
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
  • Patent number: 9593356
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Grant
    Filed: May 15, 2014
    Date of Patent: March 14, 2017
    Assignee: Takara Bio USA, Inc.
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
  • Publication number: 20150315252
    Abstract: The present invention relates to a microvesicle comprising: (i) a membrane-associated protein comprising at least one first dimerization domain, (ii) a carrier protein comprising at least one second dimerization domain, and (iii) a solute that binds to the carrier protein, wherein the solute is selected from the group of: DNA, RNA, protein, carbohydrate, ribosomes, mitochondria, and small molecules. Also provided are cells, reagents and kits that find use in making the microvesicles, as well as methods of using the microvesicles, e.g.
    Type: Application
    Filed: June 11, 2015
    Publication date: November 5, 2015
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Montserrat Morrell, Andrew Alan Farmer
  • Publication number: 20140364588
    Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
    Type: Application
    Filed: May 15, 2014
    Publication date: December 11, 2014
    Inventors: Michael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer
  • Publication number: 20030049712
    Abstract: Methods and compositions for detecting the presence of an active protease in a cell are provided. A feature of the subject methods is that a protease detection fusion protein is employed to detect the protease activity of interest. The protease detection fusion protein includes first and second subcellular localization domains separated by a protease cleavage domain, where the first subcellular localization domain is dominant over the second. The protease detection fusion proteins employed in the subject methods are further characterized by having a label domain located between the protease cleavage and second subcellular localization domains. In practicing the subject methods, the protease detection fusion protein is first provided inside the cell to be assayed. Following a suitable incubation period, the subcellular location of the label domain is determined, where the location is indicative of whether or not the protease activity of interest is present in the cell.
    Type: Application
    Filed: July 30, 2002
    Publication date: March 13, 2003
    Inventor: Michael Haugwitz