Abstract: Localized temperature control in a thermal cycler is achieved by thermoelectric modules that are protected from exposure to atmospheric moisture by a pair of loop-shaped gaskets that seal off an enclosure formed by the sample block, the heat sink, and a support frame to which the components are secured. The heat sink is a block with a plurality of fins and is secured to the thermoelectric modules by one or more clamping bars that fit between the fins and are arranged to eliminate interference with the fin geometry and with the functional surface area of the fins. Electric leads are embedded in a molded retainer element, each lead being in the shape of a “U” with two exposed legs joined by a bar at one end, one of the leads extending into the region sealed from the atmosphere and the other extending outside the region.

Abstract: Disclosed herein is a heat-conducting sample block that includes a top plate and a base plate, each having upper and lower faces, the upper face of the top plate having a recess therein. The recess has an opening for accepting a sample or sample vessel, and the lower face of the top plate has a projection extending towards and fixedly engaged with a notch on the upper face of the base plate.