Abstract: The present invention provides methods for providing a prognosis for lung cancer using a panel of eleven molecular markers that includes BAG1, BRCA1, CDC6, CDK2AP1, ERBB3, FUT3, IL11, LCK, RND3, SH3BGR, and WNT3A, which are differentially expressed in lung cancer. The eleven markers are related to patient prognosis to 5-year overall survival outcomes, and are particularly useful in providing a prognosis for non-squamous NSCLC.

Abstract: A resilient cantilevered athletic flooring system wherein a multiplicity of flexible rectangular panels having an anchor strip secured under the panels along the longitudinal centerline of the panels and multiple nailer strips attached to the top of the panels and to either side of the centerline and multiple resilient pads secured to the underside of the panel under the nailer strips and the panels are secured to a prepared base by passing anchors through the panels and the anchor strips. Multiple panels are secured side by side and end to end to cover the flooring venue and a wear floor is secured to the nailer strips to complete the installation.

Abstract: The present invention provides methods for providing a prognosis for lung cancer using a panel of eleven molecular markers that includes BAG1, BRCA1, CDC6, CDK2AP1, ERBB3, FUT3, IL11, LCK, RND3, SH3BGR, and WNT3A, which are differentially expressed in lung cancer. The eleven markers are related to patient prognosis to 5-year overall survival outcomes, and are particularly useful in providing a prognosis for non-squamous NSCLC.

Abstract: A remediation system comprises a primary scalping screen, a primary pump and sump, a large diameter maximum density separator with flocculant injection ring, a secondary pump and sump, a small diameter maximum density separator with flocculant injection ring, a static sieve screen, a flocculation sump, a vibrating screen, a dewatering device, a tertiary sump and pump, a secondary flocculation tank, and a pugmill. Screens move water and particulates through the components. In this manner larger particles are separated from smaller particles and the particles are further dewatered.

Abstract: The present invention concerns methods and means for selective inhibition of vascular smooth muscle cell (VMSC) proliferation, without negative impact on the proliferation of endothelial cells. In particular, the invention concerns the inhibition of VMSC proliferation without substantial inhibition of endothelial cell proliferation or function by delivery to a blood vessel in need of healing an E2F decoy oligonucleotide.

Abstract: In general, the invention features methods for delivering a composition to the liver in a non-surgical, percutaneous approach by utilizing the portal vein. Also disclosed are methods allowing for the liver-specific delivery of a composition.

Abstract: A method for implanting cells onto a prosthesis includes the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 &mgr;m, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 &mgr;m, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. A method for obtaining an endothelial cell culture from a blood sample involved:(a) obtaining a sample for mononuclear cells from a blood sample; and (b) culturing the sample of mononuclear cells, without further cell separation, on a cell adhesive polymer-coated solid support in the presence of endothelial growth factors.

Abstract: The invention provides a method and an apparatus for treating a tissue, such as a blood vessel, with therapeutic liquids, such as solutions of genes encoding therapeutic proteins.

Abstract: Disclosed herein is a method for implanting cells onto a prosthesis, including the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 &mgr;m, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 &mgr;m, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. Also disclosed herein are methods for culturing cells for implantation.

Abstract: Disclosed herein is a method for implanting cells onto a prosthesis, including the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 &mgr;m, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 &mgr;m, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. Also disclosed herein are methods for culturing cells for implantation.

Abstract: The present invention is directed to an isoform of the p53 tumor suppressor and to polynucleotides that encode this isoform. The isoform may be used as a marker to indicate that cardiac cells have experienced hypoxia, as would occur during a myocardial infarction. In addition, vectors encoding the isoform may be transfected into cells as a means of regulating proliferation.

Abstract: A method of enhancing injury-induced revascularization of a tissue as treatment of a disease, such as coronary artery disease, is described. The method involves (i) creating injury in a tissue (e.g., muscle, such as cardiac muscle) by, for example, use of a laser, an ultrasonic device, or a Thermal probe, and (ii) injecting into the tissue a revascularization-promoting molecule or a nucleic acid molecule encoding a revascularization-promoting molecules Also described is an apparatus that can be used to practice this method.

Abstract: In general, the invention features methods for delivering a composition to the lung in a non-surgical, percutaneous approach by utilizing the pulmonary vasculature (i.e., the pulmonary arteries and veins).Also disclosed are methods allowing for the lung-specific delivery of a composition, as well as a catheter that includes a deployable arm.

Abstract: Naked nucleic acids (DNA, RNA, and/or analogs), drugs, and/or other molecules in an extracellular environment enter cells in living intact tissue upon application of pressure to the cells and extracellular environment. Nucleic acids localize to the cell nuclei. Transfection efficiencies greater than 90% are achievable for naked DNA and RNA. A sealed enclosure, defined by an enclosing means and/or tissue, contains the cells and their extracellular environment. The enclosure is pressurized to an incubation pressure on the order of atmospheres. A protective inelastic sheath may be used to prevent distension and trauma in tissue that is part of the enclosure boundary. Suitable enclosures include pressurization chambers and organs such as blood vessels or the heart. Parts of organs, entire organs, and/or entire organisms are pressurized. Suitable target tissue types include blood vessel (in particular vein) tissue, heart, kidney, liver, and bone marrow tissue.

Abstract: The invention presents a system for inducing cells in living intact tissue, in vivo or ex vivo, to accept nucleotides from their extracellular environment and to localize those nucleotides into the cells' nuclei. This system relies on the fact that, when subjected to high pressure, cells take in nucleotides and localize those nucleotides into their nuclei with a transfection rate of greater than 90% in some cases. This invention employs various techniques for placing under high pressure either cells in isolated tissue cultures, or cells in tissues still connected to a living body.

Abstract: An efficient fluid energy collection and conversion system which has energy collectors that travel both upstream and downstream in a fluid stream. Energy collectors are mounted on a rotatable hub; during each rotation, the energy collectors travel through a downstream portion and an upstream portion of the fluid. The energy collectors' shape is changed to increase their drag while traveling downstream in the fluid stream to collect energy, and their shape is changed to decrease their drag while traveling upstream in the fluid stream to conserve energy. The energy collectors' shape are changed either passively by the pressure of the fluid stream, or actively, by computer control using servos, hydraulics, gears, pulleys, or the like. The system is adjustable to varying wind conditions. Some, or all, of the collectors are deactivated when less energy collection is desired such as during very high wind conditions. Likewise, all the energy collectors are activated when the winds are light.