Abstract: Metal nanoparticles associated with a spectroscopy-active (e.g., Raman-active) analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy (e.g., surface-enhanced Raman spectroscopy).

Abstract: Metal nanoparticles associated with a spectroscopy-active (e.g., Raman-active) analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy (e.g., surface-enhanced Raman spectroscopy).

Abstract: An encapsulated surface enhanced Raman scattering (SERS) tag. The tag includes a metal core and an encapsulant, typically a glass encapsulant. The encapsulant is further derivatized with a polymer.

Abstract: Freestanding particles comprising a plurality of segments, wherein the particle length is from 20 nm to 50 ?m and the particle width is form 5 nm to 50 ?m.

Abstract: Metal nanoparticles associated with a spectroscopy-active (e.g., Raman-active) analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy (e.g., surface-enhanced Raman spectroscopy).

Abstract: Synthetic methods for the manufacture of segmented nanoparticles are described.

Abstract: A method is disclosed for the manufacture of colloidal rod particles as nanobarcodes. Template membranes for the deposition of materials are prepared using photolithographic techniques.

Abstract: Surface-enhanced Raman spectroscopy (SERS) uses nanoscale metal particles (SERS-active particles) or surface roughness to enhance the Raman signal of Raman-active analytes contacting the surface. SERS sandwich particles contain SERS-active particles sandwiching a Raman-active substance and serve as optical tags. Preferably, the particles are rod-shaped, with each layer (SERS-active and Raman-active) formed as a distinct stripe of the particle. These freestanding particles can be derivatized with surface ligands capable of associating with analytes of interest in, for example, a biological sample. The acquired Raman spectrum of the particle encodes the identity of the ligand. Because of the simplicity and intensity of Raman spectra, highly multiplexed assays are capable using SERS particles with different Raman-active species.

Abstract: Freestanding particles comprising a plurality of segments, wherein the particle length is from 10 nm to 50 &mgr;m and the particle width is form 5 nm to 50 &mgr;m.

Abstract: A method is disclosed for the manufacture of colloidal rod particles as nanobarcodes. Template membranes for the deposition of materials are prepared using photolithographic techniques.

Abstract: Synthetic methods for the manufacture of segmented nanoparticles are described.

Abstract: Segmented nanoparticles are manufactured according to different methods and in various apparatuses.

Abstract: Methods for preparing colloidal metal nanoparticles, in which seed colloids are added to a solution of reductant mixed with a solution containing a source of metal ions, include a method in which the seeds are colloidal gold nanoparticles, the source of gold ions is HAuCl4, and the reductant is NH2OH. SERS substrates are prepared by combining a colloidal gold monolayer with a solution containing a source of metal ions and a reductant such as NH2OH.

Abstract: Metal nanoparticles associated with a spectroscopy-active (e.g., Raman-active) analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy (e.g., surface-enhanced Raman spectroscopy).

Abstract: A biosensor based on complexes between biomolecule receptors and colloidal Au nanoparticles, and more specifically, colloid layers of receptor/Au complexes that can be used to detect biomolecule analytes through measuring of binding-induced changes in electrical resistance or surface plasmon resonance. Also disclosed is a method for detecting and analysing carrier-borne chemical compounds with Raman spectroscopy using an improved SERS substrate. Further disclosed is an improved method for detecting compounds in solvents using capillary electrophoresis in conjunction with Raman spectroscopy.

Abstract: The invention provides methods and reagents for the enhancement of surface plasmon resonance (SPR)-based detection assays. The methods and reagents can be used in any molecular recognition assay that uses a solid support. The invention also provides an SPR instrument that operates in imaging mode.

Abstract: The invention provides methods and reagents for the enhancement of surface plasmon resonance (SPR)-based detection assays. The methods and reagents can be used in any molecular recognition assay that uses a solid support. The invention also provides an SPR instrument that operates in imaging mode.

Abstract: Methods for preparing colloidal metal nanoparticles, in which seed colloids are added to a solution of reductant mixed with a solution containing a source of metal ions, include a method in which the seeds are colloidal gold nanoparticles, the source of gold ions is HAuCl4, and the reductant is NH2OH. SERS substrates are prepared by combining a colloidal gold monolayer with a solution containing a source of metal ions and a reductant such as NH2OH.

Abstract: Metal nanoparticles associated with a spectroscopy-active analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy.

Abstract: Bifunctional capture probes used for multiplexed assays consist of particles bearing analyte-binding moieties and pairing oligonucleotides, which hybridize to an array of surface-bound capture oligonucleotides. Capture probes are combined with a sample containing analytes of interest, extracted from the sample, and then exposed to the oligonucleotide array. Based on their pairing oligonucleotide sequences, the capture probes self-assemble at particular array locations. Bound analytes are then detected using a method, such as mass spectrometry, that can be directed toward particular array locations. Because any number and combination of capture probes can be employed, the method is flexible and able to detect analytes at very low concentrations. Additionally, the method provides the ease of detection associated with position-addressable arrays.