Abstract: The invention relates to a cartridge (1) for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet (2) for injecting a sample (15) into the cartridge (1), a blister (3) for a buffer solution (21) with magnetic markers to mark pregiven particles (16, 16?) of the sample (15), an outlet, and a fluid channel (9), the fluid channel (9) comprising a first part that connects the inlet (2) with the blister (3) and a second part that connects the first part with the outlet, wherein the second part of the fluid channel (9) comprises an enrichment zone (5) with mechanical guiding structures to focus marked particles (16, 16?) of the sample (15) in a predetermined subsection of the fluid channel (9) and a measuring zone (6) between the enrichment zone (5) and the outlet, the measuring zone (6) comprising a magnetic field sensor (14) in the predetermined subsection of the fluid channel (9) in order to provide simplified and accelerated means for measuring particles, in particular concentrations of part

Abstract: The invention relates to a device and a method for fluidic cell guidance for flow cytometry or analyte enrichment. This allows magnetically marked analytes, in particular cells (1), to be dynamically enriched and individually detected in the flow from a sample, in particular magnetoresistively. For cell guidance, guiding ridges (12) are arranged in a flow channel (100), and so, in addition to a magnetic enrichment force (10z) and the shearing force of the flow (10y), a deflecting force (10x) caused by the fluidic obstacles (12) also acts on the cells (1) to be detected.

Abstract: The invention relates to a cartridge (1) for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet (2) for injecting a sample (15) into the cartridge (1), a blister (3) for a buffer solution (21) with magnetic markers to mark pregiven particles (16, 16?) of the sample (15), an outlet, and a fluid channel (9), the fluid channel (9) comprising a first part that connects the inlet (2) with the blister (3) and a second part that connects the first part with the outlet, wherein the second part of the fluid channel (9) comprises an enrichment zone (5) with mechanical guiding structures to focus marked particles (16, 16?) of the sample (15) in a predetermined subsection of the fluid channel (9) and a measuring zone (6) between the enrichment zone (5) and the outlet, the measuring zone (6) comprising a magnetic field sensor (14) in the predetermined subsection of the fluid channel (9) in order to provide simplified and accelerated means for measuring particles, in particular concentrations of part

Abstract: The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined. Also, the signal noise ratio can be determined using the measurement deviation amplitude.

Abstract: For flow cytometric measurement, a channel includes a magnetic sensor and is disposed downstream of a chamber. The chamber and the channel form a closed system, wherein an axis of the channel extends along a flow direction of the channel. A closed system is present if the chamber merges directly into the channel. A magnet, more particularly a permanent magnet, and a deflection device, are both arranged at a predefined side of the channel. Here, the deflection device has at least one segment. Each segment is arranged in a concentration region of the channel. Each segment has a guide for guiding cells toward the axis. The deflection device thus enables an enrichment of magnetically marked cells, which are pulled to the channel side by the magnet in the respective concentration region, on the axis and guidance of these cells along the axis to the magnetic sensor.

Abstract: The embodiments relate to an arrangement for quantifying cells of a cell suspension and enriching marked cells. The arrangement includes a fluid channel for routing the cell suspension with a first cross-section and a magnetic sensor on the fluid channel for counting magnetically marked cells in the cell suspension. The fluid channel has an enrichment region with a second cross-section which is larger than the first cross-section, a magnet being arranged on at least one side of the enrichment region.

Abstract: The embodiments relate to an arrangement for quantifying cells. The arrangement includes a magnetic field-sensitive sensor having a first and second pair of sensor elements. The sensor elements of the first pair are connected as part of a Wheatstone bridge and have a first spacing of between half and double a first average size of a first cell or cell conglomerate type. The sensor elements of the second pair are connected as part of a Wheatstone bridge and have a second spacing of between half and double a second average size of a second cell or cell conglomerate type. A third spacing of the two closest sensor elements of the pairs is greater than the larger of the two average sizes. The arrangement also includes a channel for conducting the cell suspension past the sensor elements.

Abstract: Devices and methods for magnetic flow measurement are provided. Individual analytes are detected in the through-flow, and a dynamic detection of a changing analyte state is carried out, for example with respect to the analyte size or morphology. For this purpose, the analytes to be detected, such as cells for example, are directly marked in the medium surrounding said analytes with magnetic labels and transported through the flow channel of a measuring device comprising at least two magnetic sensors. A characteristic measurement signal is generated by means of the magnetic sensors that are mutually spaced in the flow direction. The magnetic analyte diameter is calculated using the interval between the measurement deflections, and the analyte state can be evaluated using the magnetic analyte diameter.

Abstract: In a magnetic flow measurement, such as flow cytometry, individual analytes are detected in the through-flow. The analytes (e.g., cells) are marked with magnetic labels directly in the medium surrounding the analytes. The analytes are transported through the flow channel of a measuring device including at least one magnetic sensor. Using the magnetic marking of the analytes, the magnetic analyte diameter (rmag) is detected rather than the optical or hydrodynamic size (ropt) of the analytes. The analyte diameter is determined by the stray field maximum. The analyte diameter is smaller than the analyte size, such that individual analytes may be detected at high analyte concentrations.

Abstract: The invention relates to a device and a method for fluidic cell guidance for flow cytometry or analyte enrichment. This allows magnetically marked analytes, in particular cells (1), to be dynamically enriched and individually detected in the flow from a sample, in particular magnetoresistively. For cell guidance, guiding ridges (12) are arranged in a flow channel (100), and so, in addition to a magnetic enrichment force (10) and the shearing force of the flow (10), a deflecting force (10) caused by the fluidic obstacles (12) also acts on the cells (1) to be detected.

Abstract: The invention relates to an apparatus and a method for magnetic flow cytometry, wherein magnetic units (22, 24) are arranged in a flow channel (10) which is configured, with respect to the channel diameter (100) and the surface condition of the channel inner wall, in such a manner that a flow of a complex suspension can be produced in the flow channel (10) with a laminar flow profile (40). The forces (FM) that can be caused by the magnetic units (22, 24) and the forces (FS) that can be caused by the flow, applied to magnetic markers (26) that are not bound to cells, have the effect of holding back said magnetic markers (26) that are not bound to cells in the front channel section (240) and preventing them from continuing to flow along the flow channel (10) via the cell measuring device (20).

Abstract: Cells of a cell suspension are labeled by providing a microfluidic chamber having superparamagnetic labeling particles that are concentrated exactly at one inner surface of the chamber and charging the cell suspension into the chamber.

Abstract: In a measuring device, a production thereof, and a use thereof for magnetic flow cytometry, a microfluidic channel is disposed along an enrichment route such that a magnetically marked cell sample flowing through the microfluidic channel is aligned to magnetic guide strips, enriched by the magnetic field of a magnet at the floor of the channel, and guided past a sensor. The enrichment route is thereby implemented with the microfluidic channel on the packaging of the semiconductor chip carrying the sensor. This construction ensures a long enrichment route for high throughput of large sample volumes.

Abstract: A measuring device for magnetic flow cytometry has a microfluidic channel disposed along an enriching route such that a magnetically marked cell sample flowing through the microfluidic channel is aligned to magnetic guide strips, enriched by the magnetic field of a magnet at the floor of the channel, and guided past a sensor. The sensor and the magnetic guide strips are integrated on a semiconductor chip.

Abstract: Specific labeling of cells enables magnetic cell detection. The cell type to be detected is labeled, magnetic labels being bound to epitopes of a first cell-specific epitope type via antibodies of a first antibody type. Additionally, second/further magnetic labels are bound to epitopes of a second cell-specific epitope type on the cells via antibodies of a second antibody type, or the magnetic labels are bound to the antibodies of the first antibody type via antibodies of another antibody type and the antibodies of the first antibody type are bound to the epitopes of the first cell-specific epitope type on the cells.

Abstract: The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined.

Abstract: A magnetic flow cytometry apparatus for detection of cells labeled with magnetic nanoparticles has at least one pair of oppositely oriented magnets to provide between the magnets a first magnetic field region with a low magnetic field strength and to provide at poles of the magnets second magnetic field regions with a high magnetic field strength. The magnetic labeled cells provided within a flow input into the magnetic flow cytometry apparatus are enriched in at least one of the second magnetic field regions and supplied to the first magnetic field region, where a magnetic field is applied to the enriched magnetic labeled cells to measure the magnetic relaxation of the magnetic labeled cells in response to the applied magnetic field.