Abstract: The present invention relates to the field of nucleic acid collection and storage, and in particular to the collection and long-term storage of nuclei. The invention provides a device and method which can be used to capture and store nuclei at ambient temperatures allowing the subsequent isolation of nucleic acids by passive washing with a wash buffer. The present invention also provides a kit including the device of the invention suitable for carrying out the method of the invention.

Abstract: A method of performing data management in a high-speed data environment. The method includes collecting time-series information including multiple data types captured concurrently, and storing the collected time-series information in a process historian with organization, the organization occurring when the multiple data types are captured.

Abstract: The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (PCR).

Abstract: The present invention relates to the field of nucleic acid collection and storage, and in particular to the collection and long-term storage of nuclei. The invention provides a device and method which can be used to capture and store nuclei at ambient temperatures allowing the subsequent isolation of nucleic acids by passive washing with a wash buffer. The present invention also provides a kit including the device of the invention suitable for carrying out the method of the invention.

Abstract: A method of performing data management in a high-speed data environment. The method includes collecting time-series information including multiple data types captured concurrently, and storing the collected time-series information in a process historian with organization, the organization occurring when the multiple data types are captured.

Abstract: The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (PCR).

Abstract: Disclosed is a biological sample holder, a container for storing multiple holders, and an automated holder retrieval system. The sample holder may comprise an elongate generally planar support including a handle end and an opposing biological sample storage end, wherein, a substrate for accepting a biological sample is mounted to the holder at said sample storage end. Optionally the substrate is so mounted within a recess in the surface of the support and the recess has a local surface providing a reaction surface for allowing a sub-sample to be removed from the medium by mechanical action, such as cutting or punching. The container may include an array of wells each well being generally square or rectangular in plan and the holders dimensioned so that they are held diagonally in a respective well.

Abstract: Disclosed is a method for cell storage on beads and subsequent utilisation of cryogenically stored cells in cellular assays.

Abstract: The invention relates to a modified spin column for the isolation and purification of plasmid DNA. A pre-filtration disc is included in a traditional spin column. During plasmid DNA isolation, the lysate can be loaded directly to the modified spin column, eliminates the need to first remove the flocculants containing cellular debris. This results in a much shortened process. Variation of the invention includes a depth filter in between the pre-filtration disc and the main separation matrix. Also provided are kits for isolation of plasmid DNA including the modified spin columns.

Abstract: Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.

Abstract: Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei each while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.

Abstract: A device for capturing a component present in a fluid is a pipette tip having a rearward end 15 adapted to be fitted on a micro-pipette, and a forward end 12 with a membrane 17, adapted to bind the component, extending across the pipette tip.

Abstract: A method of preparing nucleic acids by obtaining an impure nucleic acid preparation, treating said preparation with phenol and adding a cyclodextrin to the treated preparation to neutralize the phenol.