Abstract: The present invention generally relates to the provision of means and methods for preparing protein compositions. The invention provides a method comprising the steps of (i) contacting a starting composition comprising a protein with a hydrolase inhibitor, wherein the hydrolase inhibitor is immobilized on a solid carrier; (ii) recovering a composition comprising the protein. Furthermore, the invention relates to a method for preparing a protein formulation comprising the steps of (i) contacting a starting composition comprising a protein with a hydrolase inhibitor, wherein the hydrolase inhibitor is immobilized on a solid carrier; (ii) recovering a composition comprising the protein, wherein the method for preparing the protein formulation further comprises adding a surfactant, preferably a fatty acid ester, to the composition comprising the protein.

Abstract: Herein is reported a method for determining the presence of hydrolase activity in a sample by incubating the sample in a buffered solution, which comprises an artificial hydrolase substrate that comprises an ester bond covalently linking an alcohol residue, which is conjugated to a label for removal, and a carboxylic acid residue, which is conjugated to a capture and detection label, as well as bovine serum albumin, and thereafter determining or quantifying, respectively, the released carboxylic acid in the free alcohol depleted incubation mixture.

Abstract: An optical sensor includes a detector having a printed circuit board (PCB) and an inductive loop. The printed circuit board (PCB) has a photodiode cathode pad with a photodiode. The inductive loop is arranged around at least part of the photodiode cathode pad, and configured to receive inductive loop inducing signaling, and provide inductive loop signaling around the at least part of the photodiode cathode pad to provide inductive on the PCB to reduce or substantially eliminate unwanted electrical interference in electrical photodiode signaling provided from the photodiode. The inductive loop includes a trace and at least one via. The trace has a route along a signal path from a transistor collector pin around an LED anode pad. The at least one via is placed between LED anode pads to route the trace on a top side of the PCB, and the trace is routed alongside, near and around the photodiode cathode pad back to the LED anode of the LED anode pad.

Abstract: An optical sensor includes a detector having a printed circuit board (PCB) and an inductive loop. The printed circuit board (PCB) has a photodiode cathode pad with a photodiode. The inductive loop is arranged around at least part of the photodiode cathode pad, and configured to receive inductive loop inducing signaling, and provide inductive loop signaling around the at least part of the photodiode cathode pad to provide inductive on the PCB to reduce or substantially eliminate unwanted electrical interference in electrical photodiode signaling provided from the photodiode. The inductive loop includes a trace and at least one via. The trace has a route along a signal path from a transistor collector pin around an LED anode pad. The at least one via is placed between LED anode pads to route the trace on a top side of the PCB, and the trace is routed alongside, near and around the photodiode cathode pad back to the LED anode of the LED anode pad.

Abstract: The present disclosure provides purification platforms comprising a depth filter step and/or a hydrophobic interaction chromatography (HIC) step. Also disclosed herein are methods of using the purification platforms described herein and compositions obtained therefrom, such as pharmaceutical compositions.

Abstract: Herein is reported a method for purifying a protein from a sample comprising the protein and at least one impurity with hydrolytic activity, comprising the following steps: i) a) applying the sample comprising the protein and at least one impurity with hydrolytic activity to a chromatography material, b) applying a solution which comprises a polysorbate to the chromatography material, and c) recovering the protein from the chromatography material, or ii) a) adding a polysorbate to the sample comprising the protein and at least one impurity with hydrolytic activity, b) applying the mixture of step a) to a chromatography material, and c) recovering the protein from the chromatography material, or iii) a) adding a polysorbate to the sample comprising the protein and at least one impurity with hydrolytic activity, b) applying the mixture of step a) to a chromatography material, c) applying a solution which comprises a polysorbate to the chromatography material, and d) recovering the protein from the chr