Abstract: Methods and microorganisms for improved malonyl-CoA flux and production of products having malonyl-CoA as a precursor. The methods comprise dynamically regulating, in a stationary phase of a method, a nitrogen regulatory protein. The methods may dynamically regulate more than one gene.

Abstract: The present disclosure relates to methods and systems for automatically providing a suggestion to delay sending a message that is being composed by a user to send to a chat participant using a chat feature of an application. The suggestion is sent in response to determining that one or more trigger conditions occurred. The suggestion includes a scheduled time to send the message. The methods and systems send the delayed message at the scheduled time.

Abstract: A facility for causing the refill of prescriptions is described. The facility conducts a conversational exchange with a person. From the exchange, the facility discerns (1) an intent of refilling a prescription, and (2) an identification of a prescription to be fulfilled. In response to this discerning, the facility causes a pharmacy refill order to be placed on behalf of the person for the identified prescription.

Abstract: An apparatus for separating, controlling, and directing a lift cord or lift chain of an architectural opening includes a body structure having first and second upper openings that are separated by an upper medial guide member that protrudes beyond the upper openings A rotatable wheel within a cavity of the body structure is configured to engage a lift cord or lift chain provided as a loop, wherein such engagement allows free passage of first and second segments of the loop through the cavity in opposing directions, while preventing free passage of the first and second segments through the cavity in the same direction. The apparatus may be coupled to a top end of a cord channel enclosure having a slider moveably engaged thereto to actuate the lift cord or lift chain, with a fixed length loop portion of lift cord or lift chain extending upward from the apparatus.

Abstract: A vehicle suspension system includes a wishbone-shaped linkage having a base, with two limbs extending from the base. Each limb is configured to support a laterally and longitudinally oriented bushing assembly, which may include a bushing assembly received by a laterally and longitudinally oriented mounting tube associated with a free end of the limb. The base includes upper and lower plates wrapping only partially around a laterally extending cross tube. A modular bushing assembly including a pair of bushing lobes and a bar pin is partially received within the cross tube, with the bushing lobes being adjustably positioned on the bar pin to allow for adjustment of the conical rate of the bushing assembly. A mounting assembly includes a pair of mount blocks secured to a vehicle axle, with the bar pin being secured with respect to the axle via the mount blocks, thereby associating the linkage to the axle.

Abstract: A device, including a hydrophilic layer on a portion of an inner surface of a transparent polymer forming a body; wherein the hydrophilic layer includes a sulfonated inner surface of the transparent polymer, a silica, a silicon oxycarbide, an O2 plasma treatment of the transparent polymer, or a combination thereof. The device can be a nebulizer or a spray chamber, for example used in an inductively coupled plasma device. A method of making the device is also disclosed.

Abstract: A dielectric cover for an insulator, supporting a high voltage conductor in an electrical distribution system, has holes for receiving retaining pins. The retaining pins are used to secure the cover over the insulator and conductor. Arched fenders, which may be circular or have a bottom opening, extend out from the cover and overlie the holes and ends of the pins. The fenders prevent birds roosting on the pin, increase the dielectric surface length between the top of the cover and the electrical conductor above the pins, and protect the pins from contamination issues which would reduce the dielectric properties of the pins. Contamination issues include birds defecating on the pins, which can create a conductive path between the bird and the inside of the cover, and atmospheric moisture such as rain, snow, and ice on the pins. Thus, wildlife is protected from electrocution.

Abstract: Provided are one or more black soldier fly (Hermetia illucens) larvae frass formulations and combinations comprising said formulations and at least one other substance including but not limited to soil amendment, fertilizer, herbicide, pesticide, fungicide, nematicide or bactericide, uses for said formulations and combinations and methods of production of said formulations.

Abstract: A scaling tool for replicating filtration characteristics of a large-scale filtration device, the scaling tool comprising a housing having a fluid inlet and a fluid outlet, and a filter receiving region in fluid communication with the fluid inlet and the fluid outlet and configured to contain a membrane comprising one or more pleats, the filter receiving region being defined by a rigid wall framework configured to hold the one or more pleats of the membrane and compress the pleats in an amount effective to replicate the filtration performance of the large-scale filtration device. Also disclosed is a methodology for configuring a filter receiving region in a scaling tool.

Abstract: Systems and methods are described for providing instant voice communications to users of a chat platform. An example method includes: providing a chat platform for a plurality of users, the chat platform including a plurality of chatrooms; presenting, on a client device of a first user from the plurality of users, a graphical user interface corresponding to a first chatroom from the plurality of chatrooms, the graphical user interface including a selectable element for initiating an instant voice communication channel among members of the chatroom, the members including the first user and at least one other user from the plurality of users; receiving, on the client device of the first user, an indication that the user has selected the selectable element in the graphical user interface; and providing, in response to the selection, the instant voice communication channel to client devices associated with the members.

Abstract: In one embodiment, a fuse cutout cover has an integral roof portion. The roof portion covers the energized top of a fuse in a first type of cutout. An attachable roof extension covers the energized top of a fuse in a larger second type of cutout. By having the optional roof extension feature, the same cover may be used with two types of cutouts. The roof extension is connected using a cruxiform connector on the bottom surface of the roof portion that tightly fits into a hole in the roof extension. To prevent an air gap between the cruxiform connector and the hole in the roof extension, the bottom of the hole is sealed. This prevents flashovers through the air gap. As a result, electrical insulation between wildlife and the energized cutout is increased.

Abstract: Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

Abstract: Certain embodiments of the present disclosure relate to a system and method for the assignment of access rights and an NFT to a user device. The method includes assigning a non-fungible token (NFT), linked with the access rights, to the account associated with the user device based on the assignment of the access rights from the list of unfilled access rights. The method further includes verifying the validity of the access rights linked with the NFT, when the user device is requesting access to the resource, based on scanning information provided on the access rights and referencing a transaction history of the NFT in the blockchain, and transmitting at least one updated NFT and privilege to access the resource to the user device based on the verification of the access rights linked with the NFT.

Abstract: A plastic retaining pin for being inserted through a retaining hole in a high voltage insulating cover has a compressible nose, with the wide part of the nose being larger than the retaining hole. The pin may be on the order of 3-12 inches long. The other end of the pin has a grasping ring for receiving the hook of a hot stick. Along the length of the body of the pin is a plurality of radial portions (e.g., six or more) extending outward from a centerline of the body. These radial portions may be skirts or ribs having a diameter less than the retaining hole in the cover. The skirts or ribs greatly increase the surface leakage distance along the body of the pin and also prevent conductive liquids flowing along the body, which may lead to a flashover.

Abstract: A method for coordinating Wireless Avionics Intra-Communication (WAIC) communications with a radio altimeter signal includes monitoring a frequency band and recording a sequence of time stamps, each time stamp of the sequence of time stamps corresponding to a time at which a strength of the radio altimeter signal exceeds a threshold signal strength in the frequency band. The method further includes calculating time stamp intervals between successive time stamps to produce a sequence of time stamp intervals, and identifying a pattern of time stamp intervals in the sequence of time stamp intervals. The method further includes coordinating the WAIC communications with the pattern of time stamp intervals to avoid interference with the radio altimeter signal.

Abstract: Systems and methods for communication between heterogenous processors via a virtual network interface implemented via programmable hardware and one or more buses. The programmable hardware may be configured with a multi-function bus such that the programmable hardware appears as both a network device and a programmable device to a host system. Additionally, the programmable hardware may be configured with a second bus to appear as a network device to an embedded system. Each system may implement network drivers to allow access to direct memory access engines configured on the programmable hardware. The configured programmable hardware and the network drivers may enable a virtual network connection between the systems to allow for information transfer via one or more network communication protocols.

Abstract: Provide herein are engineered microbial strains having greatly improved expression of many challenging protein product. This system relies on controlled expression or dynamic reduction in activity of key housekeeping proteases. Dynamic control, implemented with CRISPR based gene silencing and or controlled protein degradation enables increased protein expression while minimizing the negative impact of complete deletions of these housekeeping enzymes.

Abstract: Improved production of recombinant proteins in E. coli, reliant on tightly controlled autoinduction, triggered by phosphate depletion in stationary phase. The process also provides an optimized autoinduction media, enabling routine batch production at various culture volumes where cells densities routinely reach ˜5-7 g cell dry weight per liter and offer protein titers above 2 g/L. The methodology has been validated with a set of diverse heterologous proteins and is of general use for the facile optimization of routine protein expression from high throughput screens to fed-batch fermentation.

Abstract: Methods, devices, and systems for an unmanned system (US) for safety device testing are described herein. In some examples, one or more embodiments include a processor and a memory having instructions stored thereon which, when executed by the processor, cause the processor to capture, using an imaging device of the US, an image of a safety device, determine information corresponding to the safety device based on the image, communicate the determined information to a fire system network, receive, from the fire system network, a test procedure associated with the safety device, perform the test procedure on the safety device, and communicate a result of the test procedure to the fire system network.

Abstract: In one embodiment, a fuse cutout cover has an integral roof portion. The roof portion covers the energized top of a fuse in a first type of cutout. An attachable roof extension covers the energized top of a fuse in a larger second type of cutout. By having the optional roof extension feature, the same cover may be used with two types of cutouts. The roof extension is connected using a cruxiform connector on the bottom surface of the roof portion that tightly fits into a hole in the roof extension. To prevent an air gap between the cruxiform connector and the hole in the roof extension, the bottom of the hole is sealed. This prevents flashovers through the air gap. As a result, electrical insulation between wildlife and the energized cutout is increased.