Abstract: Disclosed are compositions and methods for proximity-mediated rolling circle amplification and for real-time detection of proximity-mediated rolling circle amplification products. Rolling circle amplification (RCA) refers to nucleic acid amplification reactions involving replication of a circular nucleic acid template to form a long strand with tandem repeats of the sequence complementary to the circular template. In proximity-mediated RCA, binding guide conjugates are brought into close proximity, generally by associating them to the same analyte or to two analytes in close proximity. The binding guide conjugates comprise a specific binding molecule and a guide oligonucleotide. The guide oligonucleotides are complementary to guide complement portions on half circle probes.