Abstract: A multi-chamber test tube which includes a first and a second matched separate elongate tubular member, means to connect the members together defining a test tube means having a dual mouth, and a stopper having a first leg and a second leg extending from the stopper body and receivable within the first and second elongate tubular member and wherein a pathway is provided from a central cavity in the body of the stopper through each of the legs and wherein a one-way valve is provided in each of the leg paths which is normally closed yet yieldable to permit flow through the leg paths in response to an elevated pressure in the cavity relative to the interior of the test tube.