Abstract: Provided herein are compositions aid methods for barcoding mammalian cells. The compositions and methods provided herein further provide methods for tracing such barcoded cells ex vivo or in vivo during the life time of an organism. In one aspect, a method of forming a barcoded cell is provided. The method includes expressing in a cell a heterologous cleaving protein complex including a sequence-specific DNA-binding domain and a nucleic acid cleaving domain. The sequence-specific DNA-binding domain targets the nucleic acid cleaving domain to a genomic nucleic acid sequence, thereby forming a genomic nucleic acid sequence bound to the heterologous cleaving protein complex.

Abstract: An improved combined cycle power plant (10) having a plurality of kettle boilers (30,31,32) used in sequence for cooling the compressed air being directed via line (29) to cool portions of the gas turbine (20). The kettle boilers remove heat from the compressed air and produce a plurality of steam flows in lines (70,71,73) at pressures in parallel with the heat recovery steam generator (41,42,43) steam flows. Steam in line (72) for cooling other portions of the gas turbine is maintained at a very high level of purity by providing a high rate of blowdown through line (65) from the steam drum (45) providing the steam. Heat from the blowdown flow is directed to heat exchanger (66) to heat incoming fuel gas from source (26), or alternatively to heat the condensate via line (74) during periods of fuel oil operation.