Abstract: Provided herein are genetically engineered cells containing one or more modulated metabolic genes, where the expression of the modulated metabolic gene(s) can be greater than that of an unmodified control. Also provided herein are methods of making the genetically engineered cells using synergistic activation mediator CRISPR-Cas9. Further provided herein are high throughput assays that can employ the genetically engineered cells provided herein.

Abstract: A document management system for managing a plurality of documents includes a document intersection database, and a document manager in communication with the document intersection database. The document intersection database defines a document intersection space for the documents. One of the document intersection spaces defines document architecture data and/or document content data of one of the documents and at least one other document associated with the one document. The architecture data defines a document architecture of the associated document, whereas the content data defines content for the associated document. The document manager is configured to update the one document intersection space with document data received from the one document and to notify the other of the documents of the update to the one document intersection space.