Patents by Inventor Michael W. Hunkapiller
Michael W. Hunkapiller has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230405544Abstract: Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.Type: ApplicationFiled: March 22, 2023Publication date: December 21, 2023Inventors: Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula, Michael W. Hunkapiller, Erik Miller, Satwik Kamtekar
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Patent number: 11578364Abstract: The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.Type: GrantFiled: June 23, 2020Date of Patent: February 14, 2023Assignee: Pacific Biosciences of California, Inc.Inventors: Sassan Sheikholeslami, Michael W. Hunkapiller, Natasha Popovich, Lei Sun, Erik Miller, Satwik Kamtekar
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Publication number: 20170268051Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: September 6, 2016Publication date: September 21, 2017Inventors: Ryan T. Koehler, Kenneth J. Livak, Junko STEVENS, Francisco M. DE LA VEGA, Michael RHODES, Laurent R. BELLON, Yu N. WANG, Xiaoqing YOU, Michael W. HUNKAPILLER, Janet S. ZIEGLE, Julie WILLIAMS, Dawn MADDEN, Dennis A. GILBERT, Charles R. SCAFE, Hadar I. ISAAC, Eugene G. SPIER, Lily XU, Leila G. SMITH, Jeremy HEIL, Stephen GLANOWSKI, John SCOTT, Emily S. WINN-DEEN, Ivy MCMULLEN, Lini WU, Harold Carey GIRE, Susan EDDINS
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Publication number: 20120303472Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: April 27, 2012Publication date: November 29, 2012Applicant: APPLIED BIOSYSTEMS, LLCInventors: Ryan T. KOEHLER, Kenneth J. LIVAK, Junko STEVENS, Francisco M. DE LA VEGA, Michael RHODES, Laurent R. BELLON, Janet S. ZIEGLE, Julie WILLIAMS, Dawn MADDEN, Dennis A. GILBERT, Charles R. SCAFE, Hadar I. Isaac, Yu N. WANG, Eugene G. SPIER, Xiaoqing YOU, Lily XU, Jeremy HEIL, Stephen GLANOWSKI, John SCOTT, Emily S. WINN-DEEN, Ivy MCMULLEN, Lini WU, Harold Carey GIRE, Michael W. HUNKAPILLER, Susan EDDINS, Leila G. SMITH
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Publication number: 20110143343Abstract: Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.Type: ApplicationFiled: July 26, 2010Publication date: June 16, 2011Applicant: Applied Biosystems, LLCInventors: MARK R. ANDERSEN, JER-KANG CHEN, MICHAEL W. HUNKAPILLER, STEVEN M. MENCHEN
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Publication number: 20100291557Abstract: Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.Type: ApplicationFiled: August 10, 2009Publication date: November 18, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kenneth J. Livak, Michael Egholm, Michael W. Hunkapiller
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Publication number: 20090239290Abstract: An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.Type: ApplicationFiled: March 16, 2009Publication date: September 24, 2009Applicant: APPLERA CORPORATIONInventors: Michael W. Hunkapiller, Dennis A. Gilbert, Kenneth J. Livak, Junko Stevens, Susan K. Eddins, Michael Lucero
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Publication number: 20090239233Abstract: An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.Type: ApplicationFiled: March 23, 2009Publication date: September 24, 2009Applicant: APPLERA CORPORATIONInventors: Michael W. Hunkapiller, Dennis A. Gilbert, Kenneth J. Livak, Junko Stevens, Susan K. Eddins, Michael Lucero
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Publication number: 20090142761Abstract: Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.Type: ApplicationFiled: April 28, 2008Publication date: June 4, 2009Applicant: Applera CorporationInventors: Mark R. Andersen, Jer-Kang Chen, Michael W. Hunkapiller, Steven M. Menchen
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Publication number: 20080228589Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: January 16, 2008Publication date: September 18, 2008Applicant: APPLERA CORPORATIONInventors: Ryan T. Koehler, Kenneth J. Livak, Junko Stevens, Francisco M. De La Vega, Michael Rhodes, Laurent R. Bellon, Julie Williams, Dennis A. Gilbert, Charles R. Scafe, Hadar I. Avi-Itzhak, Lily Xu, Susan Eddins, Michael W. Hunkapiller, Leila G. Smith
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Patent number: 7364855Abstract: Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.Type: GrantFiled: May 2, 2005Date of Patent: April 29, 2008Assignee: Applera CorporationInventors: Mark R. Andersen, Jer-Kang Chen, Michael W. Hunkapiller, Steven M. Menchen
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Patent number: 7153658Abstract: The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.Type: GrantFiled: September 19, 2003Date of Patent: December 26, 2006Assignee: Applera CorporationInventors: Mark R. Andersen, Michael W. Hunkapiller, Kenneth J. Livak, Eugene G. Spier, H. Michael Wenz
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Patent number: 7057025Abstract: Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.Type: GrantFiled: March 28, 2002Date of Patent: June 6, 2006Assignee: Applera CorporationInventors: Kenneth J. Livak, Michael Egholm, Michael W. Hunkapiller
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Publication number: 20040121371Abstract: The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.Type: ApplicationFiled: September 19, 2003Publication date: June 24, 2004Applicant: Applera CorporationInventors: Mark R. Andersen, Michael W. Hunkapiller, Kenneth J. Livak, Eugene G. Spier, H. Michael Wenz
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Publication number: 20040072195Abstract: An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.Type: ApplicationFiled: April 1, 2003Publication date: April 15, 2004Inventors: Michael W. Hunkapiller, Dennis A. Gilbert, Kenneth J. Livak, Junko Stevens, Susan K. Eddins, Michael Lucero
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Publication number: 20040063109Abstract: An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.Type: ApplicationFiled: January 2, 2003Publication date: April 1, 2004Applicant: Applera CorporationInventors: Michael W. Hunkapiller, Dennis A. Gilbert, Kenneth J. Livak, Junko Stevens, Susan K. Eddins, Michael Lucero
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Publication number: 20040018506Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: January 2, 2003Publication date: January 29, 2004Inventors: Ryan T. Koehler, Kenneth J. Livak, Junko Stevens, Francisco M. De La Vega, Michael Rhodes, Laurent R. Bellon, Janet S. Ziegle, Julie Williams, Dawn Madden, Dennis A. Gilbert, Charles R. Scafe, Hadar I. Avi-Itzhak, Yu N. Wang, Eugene G. Spier, Xiaoqing You, Lily Xu, Jeremy Heil, Stephen Glanowski, John Scott, Emily Winn-Deen, Ivy McMullen, Lini Wu, Harold Carey Gire, Susan Eddins, Michael W. Hunkapiller, Leila G. Smith
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Publication number: 20030175774Abstract: An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.Type: ApplicationFiled: January 2, 2003Publication date: September 18, 2003Inventors: Michael W. Hunkapiller, Dennis A. Gilbert, Kenneth J. Livak, Junko Stevens, Susan K. Eddins, Michael Lucero
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Publication number: 20020123046Abstract: A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. Preferably four different fragment sets are tagged with the fluorophores fluorescein, Texas Red, tetramethyl rhodamine, and 7-nitro-benzofurazan.Type: ApplicationFiled: February 2, 2001Publication date: September 5, 2002Inventors: Lloyd M. Smith, Leroy E. Hood, Michael W. Hunkapiller, Tim J. Hunkapiller, Charles R. Connell
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Patent number: RE43096Abstract: This invention provides a duplex comprising an oligonucleotide primer and a template, wherein the primer is coupled chemically to a chromophore or fluorophore so as to allow chain extension by a polymerase. In one embodiment, the primer is extended by a polymerase to generate the complement of the template. In a further embodiment, the extended primer is separated from the template for use in a number of methods, including sequencing reactions. Methods of generating these compositions of matter are further provided.Type: GrantFiled: March 13, 2003Date of Patent: January 10, 2012Assignee: California Institute of TechnologyInventors: Lloyd M. Smith, Leroy E. Hood, Michael W. Hunkapiller, Timothy Hunkapiller, Charles R. Connell