Patents by Inventor Michal Lebl
Michal Lebl has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 8252250Abstract: An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.Type: GrantFiled: November 25, 2009Date of Patent: August 28, 2012Assignees: Illumina, Inc., The Arizona Board of Regents for and on behalf of Arizona State UniversityInventors: Jonathan Posner, Kamil Salloum, Michal Lebl, Mark Reed, Dale Buermann, Matthew Hage, Bryan Crane, David Heiner, Robert Kain
-
Publication number: 20120195794Abstract: A flow cell for use in a microfluidic detection system is provided. The flow cell includes a flow cell body having a channel that is configured to convey a solution through the flow cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system, and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.Type: ApplicationFiled: April 9, 2012Publication date: August 2, 2012Applicant: Illumina, IncInventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
-
Publication number: 20120129704Abstract: Methods of generating nucleic acid fragments of substantially uniform length from sample nucleic acids comprising linearly stretching the sample nucleic acids over a substrate having a plurality of cleavage regions separated by relatively consistent distances, cleaving the linearly stretched sample nucleic acids at the cleavage regions, and collecting the resulting nucleic acid fragments. The method may further include collecting and concentrating the resultant nucleic acid fragments of substantially uniform length.Type: ApplicationFiled: April 2, 2010Publication date: May 24, 2012Applicant: Illumina, Inc.Inventors: Kevin Gunderson, Michal Lebl, David L. Heiner
-
Patent number: 8178652Abstract: A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.Type: GrantFiled: June 24, 2011Date of Patent: May 15, 2012Assignee: Illumina, Inc.Inventor: Michal Lebl
-
Patent number: 8173080Abstract: A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system, and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.Type: GrantFiled: February 14, 2008Date of Patent: May 8, 2012Assignee: Illumina, Inc.Inventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
-
Publication number: 20120012250Abstract: Disclosed herein are methods of method of making a substrate for performing a chemical synthesis reaction.Type: ApplicationFiled: September 16, 2011Publication date: January 19, 2012Inventors: Brett Ellman, Michal Lebl, Aaron Jones, Steve Fambro, David Heiner
-
Patent number: 8053553Abstract: Disclosed are compositions and methods for preventing or reducing harm resulting from pathogen infection. For example, disclosed are peptides that inhibit the processing of toxins normally cleaved by proprotein convertase enzymes.Type: GrantFiled: May 5, 2008Date of Patent: November 8, 2011Assignees: Socpra Sciences Sante Et Humaines, Illumina, Inc., Sanford-Burnham Medical Research InstituteInventors: Alex Strongin, Michal Lebl, Robert Day
-
Publication number: 20110247988Abstract: A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.Type: ApplicationFiled: June 24, 2011Publication date: October 13, 2011Inventor: Michal Lebl
-
Patent number: 8022013Abstract: A method of forming a solid-phase support, the method including the steps of providing a substrate having a reaction vessel, dispensing a particle in the reaction vessel, and permanently bonding the particle in the substrate within the reaction vessel. The particle may include a microbead. The particle may include controlled pore glass. A method of synthesis is also disclosed that includes including the steps of providing a solid-phase support including a particle embedded to the substrate adjacent a surface of substrate, the particle being functionalized to covalently attach an intermediate compound of a synthetic reaction, dispensing a liquid including a reagent to the solid-phase support to effect the synthetic reaction, and removing the liquid from the solid-phase support by centrifugation, whereby the intermediate compound remains attached to the substrate by the particle.Type: GrantFiled: August 29, 2003Date of Patent: September 20, 2011Assignee: Illumina, Inc.Inventors: Brett Ellman, Michal Lebl, Aaron Jones, Steve Fambro, David Heiner
-
Publication number: 20110178285Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.Type: ApplicationFiled: January 18, 2011Publication date: July 21, 2011Inventors: Michal Lebl, Michel Perbost, Chad F. DeRosier, Mark J. Nibbe, Steve R. Burgett, David L. Heiner
-
Patent number: 7977456Abstract: A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.Type: GrantFiled: May 3, 2004Date of Patent: July 12, 2011Assignee: Illumina, Inc.Inventor: Michal Lebl
-
Publication number: 20110143965Abstract: An apparatus for high-throughput combinatorial synthesis of organic molecules including a reaction vessel for containing a combinatorial chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis or rotation. A method of combinatorial synthesis of organic molecules is also disclosed.Type: ApplicationFiled: February 8, 2011Publication date: June 16, 2011Inventors: Michal Lebl, Mark S. Chee, Steven M. Barnard, David L. Heiner, Vit Pokorny, Mark J. Nibbe, Aaron Christopher Jones
-
Publication number: 20110136696Abstract: Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.Type: ApplicationFiled: February 16, 2011Publication date: June 9, 2011Applicant: ILLUMINA, INC.Inventors: David L. Heiner, Aaron C. Jones, Steven P. Fambro, Mark J. Nibbe, Steve R. Burgett, Brett M. Ellman, Michal Lebl
-
Publication number: 20110136684Abstract: Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using these systems.Type: ApplicationFiled: February 11, 2011Publication date: June 9, 2011Inventors: David Louis Heiner, Steven P. Fambro, Theo Kotseroglou, Michal Lebl
-
Publication number: 20110105361Abstract: A method of reading a plurality of encoded microvessels used in an assay for biological or chemical analysis. The method can include providing a plurality of encoded microvessels. The microvessels can include a respective microbody and a reservoir core configured to hold a substance in the reservoir core. The microbody can include a material that surrounds the reservoir core and facilitates detection of a characteristic of the substance within the reservoir core. Optionally, the material can be transparent so as to facilitate detection of an optical characteristic of a substance within the reservoir core. The microbody can include an identifiable code associated with the substance. The method can also include determining the corresponding codes of the microvessels.Type: ApplicationFiled: October 29, 2010Publication date: May 5, 2011Applicant: ILLUMINA, INC.Inventors: JOHN A. MOON, M. SHANE BOWEN, RYAN C. SMITH, MICHEL PERBOST, MICHAL LEBL, STEVEN H. MODIANO
-
Publication number: 20110072914Abstract: A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.Type: ApplicationFiled: February 14, 2008Publication date: March 31, 2011Applicant: ILLUMINA, INC.Inventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
-
Patent number: 7914739Abstract: Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.Type: GrantFiled: September 13, 2006Date of Patent: March 29, 2011Assignee: Illumina, Inc.Inventors: David L. Heiner, Aaron C. Jones, Steven P. Fambro, Mark J. Nibbe, Steve R. Burgett, Brett M. Ellman, Michal Lebl
-
Patent number: 7887752Abstract: Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using the these systems.Type: GrantFiled: January 21, 2004Date of Patent: February 15, 2011Assignee: Illumina, Inc.Inventors: David Louis Heiner, Steven P. Fambro, Theo Kotseroglou, Michal Lebl
-
Publication number: 20100187115Abstract: An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.Type: ApplicationFiled: November 25, 2009Publication date: July 29, 2010Applicant: ILLUMINA CORPORATIONInventors: Jonathan Posner, Kamil Salloum, Michal Lebl, Mark Reed, Dale Buermann, Matthew Hage, Bryan Crane, David Heiner, Robert Kain
-
Publication number: 20090029924Abstract: Disclosed are compositions and methods for preventing or reducing harm resulting from pathogen infection. For example, disclosed are peptides that inhibit the processing of toxins normally cleaved by proprotein convertase enzymes.Type: ApplicationFiled: May 5, 2008Publication date: January 29, 2009Inventors: Alex Strongin, Michal Lebl, Robert Day