Abstract: The present invention relates to an aqueous extract useful notably for the regeneration of human or animal cells, notably mammalian cells, comprising an aqueous extract free of any insoluble solid debris of ground planarian organisms having a regeneration capacity, containing at least the intracellular components of the cells of said organisms of a cell lysate of said cells of said organisms.

Abstract: The present invention relates to an in vitro diagnostic method with regard to the presence of bacterial vaginosis, characterized in that the presence of bacterial vaginosis is determined if the archaea Methanobrevibacter smithii is present in a patient vaginal secretion sample by detecting therein the presence of at least one nucleic acid sequence specific to said methanogenic archaea Methanobrevibacter smithii.

Abstract: The present invention relates to a method for cultivating in vitro in an acellular culture medium, bacteria for which the growth is sensitive to oxygen content, said bacterium being selected from anaerobic bacteria and intracellular micro-aerophilic bacteria, characterized in that uric acid is added into said acellular culture medium, and said bacterium is cultivated in said culture medium in the presence of oxygen.

Abstract: The present invention relates to the biological production of methane (Biogas) by co-culture in an aerobic atmosphere of a methanogenic bacterium and of an anaerobic bacterium capable of producing hydrogen, in a culture medium comprising or being supplemented with carbohydrate compound(s), notably starch and/or sugars, and supplemented with antioxidant compound(s).

Abstract: This method for detecting at least one particle in a bodily fluid is carried out via a detection system including a light source, a transparent substrate and a photodetector array, the substrate being positioned between the light source and the photodetector. This method includes the placement of a droplet of bodily fluid on the substrate, the illumination of the droplet via the light source, the acquisition of several successive images of the droplet via the photodetector, each image being formed by radiation transmitted by the illuminated droplet and including at least one elementary diffraction pattern, each elementary diffraction pattern corresponding to waves diffracted by a particle upon illumination of the droplet, the identification, via the acquired images of the mobile elementary diffraction patterns, and the counting of moving particles in the droplet, via the identified mobile elementary diffraction patterns.

Abstract: The present invention relates to the biological production of methane (Biogas) by co-culture in an aerobic atmosphere of a methanogenic bacterium and of an anaerobic bacterium capable of producing hydrogen, in a culture medium comprising or being supplemented with carbohydrate compound(s), notably starch and/or sugars, and supplemented with antioxidant compound(s).

Abstract: The present invention relates to a method for cultivating in vitro in an acellular culture medium, bacteria for which the growth is sensitive to oxygen content, said bacterium being selected from anaerobic bacteria and intracellular micro-aerophilic bacteria, characterized in that uric acid is added into said acellular culture medium, and said bacterium is cultivated in said culture medium in the presence of oxygen.

Abstract: This method for detecting at least one particle in a bodily fluid is carried out via a detection system including a light source, a transparent substrate and a photodetector array, the substrate being positioned between the light source and the photodetector. This method includes the placement of a droplet of bodily fluid on the substrate, the illumination of the droplet via the light source, the acquisition of several successive images of the droplet via the photodetector, each image being formed by radiation transmitted by the illuminated droplet and including at least one elementary diffraction pattern, each elementary diffraction pattern corresponding to waves diffracted by a particle upon illumination of the droplet, the identification, via the acquired images of the mobile elementary diffraction patterns, and the counting of moving particles in the droplet, via the identified mobile elementary diffraction patterns.

Abstract: The present invention concerns the use of a strain of a mycobacterium of the Mycobacterium tuberculosis complex in which a mspA gene capable of expressing a porin A of Mycobacterium smegmatis has been inserted, to produce a vaccine for the prevention of infection with a bacterium of the Mycobacterium tuberculosis complex in a host having eukaryote cells, preferably macrophages, the said strain of mycobacteria thus transformed having reduced growth in the said eukaryote cells.

Abstract: The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of the Mycobacterium tuberculosis complex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of the Mycobacterium tuberculosis complex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention.

Abstract: The present invention concerns a method of detection and preferably of quantification of DNA, preferably comprising prokaryotic DNA, extracted from a stool sample of an individual, especially for the molecular determination of the composition of the intestinal flora in the stool. According to the invention, one controls the quality of the DNA extraction by verifying whether one detects a specific DNA of Methanobrevibacter smithii and one performs the quantification of said specific prokaryotic DNA for Archae Methanobrevibacter smithii, for the bacterial genus Lactobacillus, for the phylum Bacteroidetes and for the phylum Firmicutes, respectively, to provide a diagnosis and/or monitoring of the weight status of an individual. The present invention provides a method for carrying out the extraction of prokaryotic DNA in stools.

Abstract: The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of the Mycobacterium tuberculosis complex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of the Mycobacterium tuberculosis complex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention.

Abstract: The invention relates to a method of detecting, by means of molecular identification, a bacterium from one of the Staphylococcus-type species. The inventive method is characterised in that the following are used: a fragment of the rpoB gene of said bacterium, comprising a nucleotide sequence selected from one of the SEQ. ID. No. 11 to 39 sequences, the reverse sequences and the complementary sequences; or an oligonucleotide comprising a sequence having at least 12 consecutive nucleotide patterns included in one of the SEQ. ID. No. 7 to 10 sequences, in which N represents a nucleotide selected from inosine and an equimolar mixture of 4 different nucleotides selected from A, T, C or G and from the oligonucleotides of the reverse sequences and complementary sequences.

Abstract: A method for the serological diagnosis, in vitro, of an infection with a spirochete bacterium which is pathogenic in humans, chosen from bacteria of the Borrelia, Leptospira and Treponema genera, by carrying out at least one of 1) an assay of IgG-type and/or IgM-type antibodies against at least one tick-borne Borrelia bacterium, chosen from Borrelia duttonii and Borrelia crocidurae, and 2) an assay of IgM-type antibodies against the louse-borne Borrelia bacterium Borrelia recurrentis.

Abstract: The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genus Streptococcus and four related genera Enterococcus, Gemella, Abiotrophia and Granulicatella which consists of using as probe or primer: the rpoB gene or gene fragment of one said bacterium of sequences SEQ ID no 1 to 3, or an oligonucleotide or mixture of oligonucleotides derived from sequences SEQ ID no 8 to 35, or in particular the oligonucleotides of sequences SEQ ID no 6 and 7.

Abstract: The invention concerns the total sequence of the Tropheryma whippelii bacteria rpoB gene, characterized in that it consists of the nucleotide sequence SEQ ID no. °9, and the nucleotide sequences derived from said sequence specific of the Tropheryma whippelii bacteria and their use as probe and primer.

Abstract: The invention concerns single stranded oligonucleotides comprising a sequence of at least 12 consecutive nucleotide motifs contained in one of the sequences SEQ ID NO: 1 to SEQ ID NO: 4 wherein “n” represents an identical or different nucleotide selected among inosine or an equimolar mixture of 4 different nucleotides selected among a, t, c or g, and among oligonucleotides complementary thereto. The invention also concerns using the oligonucleotides to detect bacteria of the Spirochaetales order. The invention is based on the use of specifically defined sequences in the rpoB gene of spirochetes coding for the beta subunit of the bacterial RNA polymerase.

Abstract: The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genus Streptococcus and four related genera Enterococcus, Gemella, Abiotrophia and Granulicatella which consists of using as probe or primer: the rpoB gene or gene fragment of one said bacterium of sequences SEQ ID no 1 to 3, or an oligonucleotide or mixture of oligonucleotides derived from sequences SEQ ID no 8 to 35, or in particular the oligonucleotides of sequences SEQ ID no 6 and 7.

Abstract: The invention relates to a method of detecting, by means of molecular identification, a bacterium from one of the Staphylococcus-type species. The invention method is characterised in that the following are used: a fragment of the rpoB gene of said bacterium, comprising a nucleotide sequence selected from one of the SEQ ID. no. 11 to 39 sequences, that reverse sequences, the reverse sequences and the complimentary sequences; or an oligonucleotide comprising a sequence having at least 12 consecutive nucleotide patterns included in one of the SEQ.ID. no. 7 to 10 sequences, in which N represents a nucleotide selected from inosine and an eqimolar mixture of 4 different nucleotides selected from A, T, C or G and from the oligonucleotides of the reverse sequences and complementary sequences.

Abstract: The invention concerns the total sequence of the Tropheryma whippelii bacteria rpoB gene, characterized in that it consists of the nucleotide sequence SEQ ID n°9, and the nucleotide sequences derived from said sequence specific of the Tropheryma whippelii bacteria and their use as probe and primer.