Abstract: The invention is directed to a method for detecting a target moiety in a sample of biological specimens by the steps a) providing at least one conjugate with the general formula (I) Xn— P—Ym, with X is a detection moiety, P an acidic or basic degradable spacer and Y an antigen or oligonucleotide recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P, b) binding the conjugate to the target moiety target via the antigen or oligonucleotide recognizing moiety Y, thereby labelling the target moiety, c) detecting the target moiety labelled with the conjugate by detecting the detecting moiety X, d) providing at least one precursor which is capable of releasing an acid or base when provided with radiation wherein the acid or base is capable of cleaving P, e) activating the precursor by providing radiation, thereby releasing the acid or base capable of cleaving P, and f) degrading spacer P with the released acid or base, thereby cleaving the detection moiety X from

Abstract: The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7) wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6 wherein R1, R2 and R3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B) wherein R4, R5 R6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF4?, F?, Cl?, Br?, I?, SO42?, SO32? in aqueous solution having a pH between 3 and 7.

Abstract: DNA sequencing by sequential addition/incorporation of non 3’capped and fluorescently labeled nucleotides. Each incorporation of individual nucleotides (A, or T or G or C) are separated by a wash. After all incorporation using all four bases, the substrate is imaged then the dyes are cleaved, and the following cycle of incorporations, washes, imaging and cleave is resumed.

Abstract: The invention is directed to a method for detecting RNA, DNA or protein target sequences by a) Hybridizing a library of probes having the general formula (I) P—(CL-D)x??(I) ?With P: probes having at least 10 nucleotides or amino acids CL: cleavable linker D: fluorescent dye X: integer between 1 and 5 ?to RNA, DNA or protein target sequences wherein the library comprises probes P having different sequences of nucleotides or amino acids and cleavable linkers CL of different groups which are cleavable with different means b) Removing unhybridized probes and detecting the hybridized probes via the fluorophores D by a first image c) Cleaving sequentially by different means each group of chemical linkers CL from the hybridized probes; removing the thus cleaved fluorophores D and detecting the remaining hybridized probes via their fluorophores D by a second image d) Detecting the removed fluorophores D by comparing the first and second image.

Abstract: The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7) wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6 wherein R1, R2 and R3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B) wherein R4, R5 R6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF4?, F?, Cl?, Br?, I?, SO42?, SO32? in aqueous solution having a pH between 3 and 7.

Abstract: The present invention is related to DNA/RNA sequencing and in particular approaches termed Next Generation Sequencing (NGS). Improved methods and integrated devices are disclosed, which enable faster and more efficient analysis of nucleic acids. One aspect of the invention has to do with integration of detection system into consumable flowcell. This enables miniaturization and simplification of sequencing instrumentation while simultaneously reducing sequencing costs. By performing sequencing and detection directly on the sensor, such as CCD or CMOS, one can significantly speed up the sequencing process.

Abstract: A kit includes an array of charge sensors attached to a solid-phase substrate , wherein individual charge sensors in the array respectively comprise a channel and a polymerase and are located at different addressable locations on the solid-phase substrate; and a liquid comprising a plurality of different nucleic acids, each of the different nucleic acids including a unique label to be detected by a charge sensor of the array.

Abstract: Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract: Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.

Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.

Abstract: A plurality of isolated microvessels including a plurality of encoded microvessels each having a microbody and a reservoir core. The microbody is configured to separate a biological or chemical substance in the reservoir core from an ambient environment surrounding the microbody. The microbody includes a transparent material that at least partially surrounds the reservoir core and facilitates detection of an optical characteristic of the substance within the reservoir core. The microbody of each microvessel includes an identifiable code that distinguishes individual microvessels of the plurality of encoded microvessels from each other. The plurality of isolated microvessels also includes a plurality of compartments each configured to separate individual microvessels of the plurality of encoded microvessels from each other.

Abstract: A plurality of isolated microvessels including a plurality of encoded microvessels each having a microbody and a reservoir core. The microbody is configured to separate a biological or chemical substance in the reservoir core from an ambient environment surrounding the microbody. The microbody includes a transparent material that at least partially surrounds the reservoir core and facilitates detection of an optical characteristic of the substance within the reservoir core. The microbody of each microvessel includes an identifiable code that distinguishes individual microvessels of the plurality of encoded microvessels from each other. The plurality of isolated microvessels also includes a plurality of compartments each configured to separate individual microvessels of the plurality of encoded microvessels from each other.

Abstract: A method of reading a plurality of encoded microvessels used in an assay for biological or chemical analysis. The method can include providing a plurality of encoded microvessels. The microvessels can include a respective microbody and a reservoir core configured to hold a substance in the reservoir core. The microbody can include a material that surrounds the reservoir core and facilitates detection of a characteristic of the substance within the reservoir core. Optionally, the material can be transparent so as to facilitate detection of an optical characteristic of a substance within the reservoir core. The microbody can include an identifiable code associated with the substance. The method can also include determining the corresponding codes of the microvessels.

Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.