Patents by Inventor Michele C. Kieke

Michele C. Kieke has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Yeast cell surface display of proteins and uses thereof
    Patent number: 6300065
    Abstract: The present invention provides a genetic method for tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. Also provided is a method for genetic fusion of the N terminus of a polypeptide of interest to the C-terminus of the yeast Aga2p cell wall protein. Attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.
    Type: Grant
    Filed: August 26, 1998
    Date of Patent: October 9, 2001
    Assignee: Board of Trustees of the University of Illinois
    Inventors: Michele C. Kieke, K. Dane Wittrup, Eric T. Boder, David M. Kranz, Eric Shusta