Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Described herein is a method for producing photochromic silicone hydrogel contact lenses in a relatively efficient and consistent manner from a polymerizable composition under a controlled thermal curing scheme. The main polymerizable components in the polymerizable composition are a high radical-reactive hydrophilic (meth)acrylamido monomer, a high radical-reactive siloxane-containing (meth)acrylamido monomer, and a polysiloxane vinylic crosslinker(s) free of low-reactive ethylenically unsaturated group as the main crosslinker. The thermal free radical initiator having a 10 hour half-life temperature (T10h?) of from about 50° C. to about 90° C. The controlled thermal curing scheme includes maintaining a first curing temperature of from about (T10h??20)° C. to about T10h?° C. for a first curing time and maintaining a second curing temperature of from about (T10h?+10)° C. to about (T10h?+35)° C. for a second curing time.

Abstract: The disclosure relates to highly temperable colored glass compositions. The colored glass compositions have high coefficients of thermal expansion and high Young's moduli that advantageously absorb in the ultraviolet and/or blue wavelength ranges. Methods of making such glasses are also provided.

Abstract: A system and method for controlling air quality within an indoor space are disclosed. An example system includes an air circulation unit that moves air through ductwork of a heating, ventilation, and air conditioning (HVAC) system and an air sanitization unit within the ductwork of the HVAC system that sanitizes air passing through the ductwork of the HVAC system. The system further includes an indoor air quality controller that controls a rate at which the air circulation unit moves the air through the ductwork of the HVAC system responsive to inputs received at the indoor air quality controller and controls an operational status of the air sanitization unit responsive to the inputs received at the indoor air quality controller.

Abstract: It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.

Abstract: Provided herein are methods for preparing and characterizing CAR-T cell cultures and preparations.

Abstract: In various embodiments single-step ATPS paper-based diagnostic assays are provided that exploit the concept of sequential resolubilization of ATPS components to give rise to the desired phase separation behavior within paper. In one illustrative embodiment, a wick is provided for concentrating an analyte within an aqueous two-phase extraction system in a paper, where the wick comprises a paper configured to receive a sample where the paper comprises a first region containing a first component of an aqueous two-phase system (ATPS) where the first component is in a dry form, and a second region containing a second component of an aqueous two-phase system (ATPS) where the second component is in a dry form; and where said first region and the second region are disposed so that when said wick is contacted with a fluid sample, the first component of said ATPS is hydrated before the second component. In certain embodiments the first and second component are disposed so they are hydrated substantially simultaneously.

Abstract: It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.

Abstract: A ball screw with a buffering circulation member comprises: a screw, a nut, a circulation member, a plurality of rolling elements and a plurality of flexible members. The circulation member comprises a first structure and a second structure. The flexible members are disposed at the combining portion between the circulation member and the nut to allow the first and second structures to be assembled more closely in a seamless manner. The flexible members which support the circulation member can absorb or buffer the collision of the rolling elements, thus reducing the vibration of the rolling elements or the noise caused by the collision of the rolling elements.

Abstract: An Internet radio infrastructure comprising at least a media server and one or more wireless portable internet radio devices and/or one or more media center devices is proposed. The internet radio infrastructure is configured for broadcasting, archiving, and playing internet radio streams in a manner that would not only provide increased flexibility and options for the users as well as simplified management for the internet radio infrastructure implementer, but would also save time and resources in the implementation and maintenance of an internet radio system.

Abstract: A ball screw with a circulation member comprises: a screw, a nut, a circulation member, a plurality of rolling elements and a plurality of flexible members. The flexible members are disposed at the combining portion between the circulation member and the nut to allow them to be assembled closely, and to prevent the circulation member and the nut from being damaged when they are assembled and disassembled.

Abstract: A peer-to-peer broadcast management system is described. A system may include a peer detector to determine one or more peer computer systems associated with a predetermined locality; a peer list generator to generate a peer list of the determined peer computer systems; a communications module to receive a request for content; and a peer selector to process the request for content utilizing the peer list.