Patents by Inventor Mikiro Hayashi
Mikiro Hayashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240148799Abstract: The present invention relates to a composition which contains an intestinal bacterium belonging to Bacteroidetes and having an inflammation reducing activity, or a substance derived from the intestinal bacterium and having an inflammation reducing activity and which has at least one activity selected from an anti-inflammatory activity, an immunoregulation activity, an epithelial barrier restoring activity, an IL-10-inducing activity, and an IL-22-inducing activity, and to an ameliorating agent for an inflammatory disease, an autoimmune disease, or an infectious disease containing the intestinal bacterium and the substance derived from the intestinal bacterium and having an inflammation reducing activity.Type: ApplicationFiled: March 8, 2022Publication date: May 9, 2024Applicants: JUNTENDO EDUCATIONAL FOUNDATION, Kyowa Kirin Co., Ltd., KYOWA HAKKO BIO CO., LTD.Inventors: Dai ISHIKAWA, Satoshi KOIZUMI, Makoto YAMASHITA, Fuhito YAMAZAKI, Mikiro HAYASHI, Nakayuki HONMA, Itaru URAKAWA, Susumu IWAMOTO
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METHOD FOR PRODUCING HEPAROSAN AND BACTERIUM OF GENUS ESCHERICHIA HAVING HEPAROSAN-PRODUCING ABILITY
Publication number: 20230175028Abstract: An object of the present invention is to provide a method for efficiently producing heparosan by improving a heparosan-producing ability through genetic modification of a bacterium of the genus Escherichia which has a heparosan-producing ability. The present invention relates to a bacterium of the genus Escherichia which has a genetic modification that increases an expression of a kpsS gene, and which has a heparosan-producing ability; and a method for producing heparosan using the bacterium.Type: ApplicationFiled: April 2, 2021Publication date: June 8, 2023Applicants: RENSSELAER POLYTECHNIC INSTITUTE, OTSUKA PHARMACEUTICAL FACTORY, INC., KIRIN BIOMATERIALS CO., LTD.Inventors: Ayano KOMINE, Mikiro HAYASHI -
Publication number: 20230159969Abstract: The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.Type: ApplicationFiled: April 2, 2021Publication date: May 25, 2023Applicants: RENSSELAER POLYTECHNIC INSTITUTE, OTSUKA PHARMACEUTICAL FACTORY, INC., KIRIN BIOMATERIALS CO., LTD.Inventors: Kenta FUKUNAGA, Aki NISHIBARA, Ryosuke KATO, Masahiro KURATSU, Mikiro HAYASHI, Shin-ichi HASHIMOTO
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Patent number: 8859241Abstract: Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus Escherichia. According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium: [1] a protein comprising the amino acid sequence shown in SEQ ID NO: 2 [2] a protein consisting of an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity [3] a protein consisting of an amino acid sequence having 80% or more homology to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.Type: GrantFiled: January 7, 2011Date of Patent: October 14, 2014Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Mikiro Hayashi, Kazuhiko Tabata
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Patent number: 8703447Abstract: Provided is an efficient process for producing L-glutamine or L-glutamic acid using a microorganism. L-glutamine or L-glutamic acid is produced by culturing in a medium a microorganism in which has an ability to produce L-glutamine or L-glutamic acid, and in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, producing and accumulating L-glutamine or L-glutamic acid in the medium, and recovering L-glutamine or L-glutamic acid from the medium.Type: GrantFiled: January 7, 2011Date of Patent: April 22, 2014Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Mikiro Hayashi, Kazuhiko Tabata, Yoshiyuki Yonetani
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Patent number: 8673590Abstract: The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to theoutside of the microbial cell is higher than that of a parental strain.Type: GrantFiled: April 4, 2008Date of Patent: March 18, 2014Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Mikiro Hayashi, Kazuhiko Tabata, Makoto Yagasaki, Yoshiyuki Yonetani
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Publication number: 20120329106Abstract: Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus Escherichia. According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium: [1] a protein comprising the amino acid sequence shown in SEQ ID NO: 2 [2] a protein consisting of an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity [3] a protein consisting of an amino acid sequence having 80% or more homology to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.Type: ApplicationFiled: January 7, 2011Publication date: December 27, 2012Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Mikiro Hayashi, Kazuhiko Tabata
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Publication number: 20120322115Abstract: Provided is an efficient process for producing L-glutamine or L-glutamic acid using a microorganism. L-glutamine or L-glutamic acid is produced by culturing in a medium a microorganism in which has an ability to produce L-glutamine or L-glutamic acid, and in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, producing and accumulating L-glutamine or L-glutamic acid in the medium, and recovering L-glutamine or L-glutamic acid from the medium.Type: ApplicationFiled: January 7, 2011Publication date: December 20, 2012Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Mikiro Hayashi, Kazuhiko Tabata, Yoshiyuki Yonetani
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Patent number: 8187843Abstract: According to the present invention, it is possible to provide a microorganism belonging to the genus Corynebacterium, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.Type: GrantFiled: August 30, 2007Date of Patent: May 29, 2012Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Yugo Adachi, Mikiro Hayashi, Akihiro Senoo, Yoshiyuki Yonetani, Shin-ichi Hashimoto
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Patent number: 8067211Abstract: A polypeptide comprising an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence of a glutamine synthetase 2 derived from a microorganism belonging to a coryneform bacterium, wherein the coryneform bacterium producing the polypeptide has L-glutamine productivity, a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism comprising the DNA or the recombinant DNA, and a process for producing L-glutamine using the microorganism are provided.Type: GrantFiled: December 27, 2006Date of Patent: November 29, 2011Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Mikiro Hayashi, Masaki Maeda, Yoshiyuki Yonetani
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Publication number: 20110124059Abstract: According to the present invention, it is possible to provide a microorganism belonging to the genus Corynebacterium, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.Type: ApplicationFiled: August 30, 2007Publication date: May 26, 2011Applicant: Kyowa Hakko Bio Co., Ltd.Inventors: Yugo Adachi, Mikiro Hayashi, Akihiro Senoo, Yoshiyuki Yonetani, Shin-ichi Hashimoto
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Publication number: 20100184163Abstract: A polypeptide comprising an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence of a glutamine synthetase 2 derived from a microorganism belonging to a coryneform bacterium, wherein the coryneform bacterium producing the polypeptide has L-glutamine productivity, a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism comprising the DNA or the recombinant DNA, and a process for producing L-glutamine using the microorganism are provided.Type: ApplicationFiled: December 27, 2006Publication date: July 22, 2010Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: Mikiro Hayashi, Masaki Maeda, Yoshiyuki Yonetani
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Patent number: 7741081Abstract: The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positions 1 to 19 or 39 to 294; and which has N-acetylglutamate kinase activity.Type: GrantFiled: September 28, 2005Date of Patent: June 22, 2010Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Masato Ikeda, Tetsuo Nakano, Satoshi Mitsuhashi, Mikiro Hayashi, Kenji Tanaka
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Publication number: 20100129867Abstract: The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to the outside of the microbial cell is higher than that of a parental strain.Type: ApplicationFiled: April 4, 2008Publication date: May 27, 2010Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Mikiro Hayashi, Kazuhiko Tabata, Makoto Yagasaki, Yoshiyuki Yonetani
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Publication number: 20090123980Abstract: The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positions 1 to 19 or 39 to 294; and which has N-acetylglutamate kinase activity.Type: ApplicationFiled: September 28, 2005Publication date: May 14, 2009Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: Masato Ikeda, Tetsuo Nakano, Satoshi Mitsuhashi, Mikiro Hayashi, Kenji Tanaka
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Patent number: 7445915Abstract: The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.Type: GrantFiled: March 19, 2003Date of Patent: November 4, 2008Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Masato Ikeda, Mikiro Hayashi, Junko Ohnishi, Satoshi Mitsuhashi, Hiroshi Mizoguchi, Satoshi Nakagawa
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Patent number: 7332310Abstract: Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.Type: GrantFiled: March 22, 2004Date of Patent: February 19, 2008Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Nakagawa, Hiroshi Mizoguchi, Seiko Ando, Mikiro Hayashi, Keiko Ochiai, Haruhiko Yokoi, Naoko Tateishi, Akihiro Senoh, Masato Ikeda, Akio Ozaki
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Publication number: 20060228712Abstract: Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.Type: ApplicationFiled: March 22, 2004Publication date: October 12, 2006Applicant: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Nakagawa, Hiroshi Mizoguchi, Seiko Ando, Mikiro Hayashi, Keiko Ochiai, Haruhiko Yokoi, Naoko Tateishi, Akihiro Senoh, Masato Ikeda, Akio Ozaki
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Publication number: 20050130276Abstract: The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.Type: ApplicationFiled: March 19, 2003Publication date: June 16, 2005Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: Masato Ikeda, Mikiro Hayashi, Junko Ohnishi, Satoshi Mitsuhashi, Hiroshi Mizoguchi, Satoshi Nakagawa
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Publication number: 20020197605Abstract: Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.Type: ApplicationFiled: December 18, 2000Publication date: December 26, 2002Inventors: Satoshi Nakagawa, Hiroshi Mizoguchi, Seiko Ando, Mikiro Hayashi, Keiko Ochiai, Haruhiko Yokoi, Naoko Tateishi, Akihiro Senoh, Masato Ikeda, Akio Ozaki