Abstract: A fluidic connection system may comprise a port which includes a capillary tube receptacle with an end seal face and a threaded wall. The fluidic connection system may also comprise a capillary tube that includes a front end face for sealingly abutting against the end seal face of the port. On the capillary tube, a flange may be affixed at a distance from the front end face of the capillary tube. The fluidic connection system may further comprise a fitting nut that includes an axial hole to allow the capillary tube to pass therethrough and a matching threaded wall corresponding to the threaded wall of the port. The fitting nut may be slidably coupled with the capillary tube behind the flange. The distance at which the flange is affixed may be predetermined such that when the flange is pushed forward by the fitting nut, the front end face of the capillary tube abuts directly against the end seal face of the port.

Abstract: A fluidic connection system may comprise a port which includes a capillary tube receptacle with an end seal face and a threaded wall. The fluidic connection system may also comprise a capillary tube that includes a front end face for sealingly abutting against the end seal face of the port. On the capillary tube, a flange may be affixed at a distance from the front end face of the capillary tube. The fluidic connection system may further comprise a fitting nut that includes an axial hole to allow the capillary tube to pass therethrough and a matching threaded wall corresponding to the threaded wall of the port. The fitting nut may be slidably coupled with the capillary tube behind the flange. The distance at which the flange is affixed may be predetermined such that when the flange is pushed forward by the fitting nut, the front end face of the capillary tube abuts directly against the end seal face of the port.

Abstract: A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel. The pressurized fluid channel may extend along an outer periphery of the low pressure fluid channel. The eluent may be received from an eluent generator at a pressure of at least about 3300 psi, and in various embodiments up to about 5000 psi. A liquid chromatography system and method are also disclosed.

Abstract: A high-pressure capillary degas assembly includes an assembly block having an eluent inlet and an eluent outlet, and a separator support extending therebetween, a wash inlet and a wash outlet and a groove extending therebetween, a tubular separator extending within the assembly block, the separator having an inner surface and an outer surface, the inner surface defining an eluent fluid line fluidly interconnecting the eluent inlet and outlet, the outer surface abutting against the separator support and enclosing the groove to define a wash line within the groove. The separator may be configured to retain liquid in the eluent fluid line at a first pressure and allow gas to flow through the separator to the wash fluid line at a second pressure lower than the first pressure. A method of using the high-pressure capillary degas assembly is also disclosed.

Abstract: A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel. The pressurized fluid channel may extend along an outer periphery of the low pressure fluid channel. The eluent may be received from an eluent generator at a pressure of at least about 3300 psi, and in various embodiments up to about 5000 psi. A liquid chromatography system and method are also disclosed.

Abstract: A high-pressure capillary degas assembly includes an assembly block having an eluent inlet and an eluent outlet, and a separator support extending therebetween, a wash inlet and a wash outlet and a groove extending therebetween, a tubular separator extending within the assembly block, the separator having an inner surface and an outer surface, the inner surface defining an eluent fluid line fluidly interconnecting the eluent inlet and outlet, the outer surface abutting against the separator support and enclosing the groove to define a wash line within the groove. The separator may be configured to retain liquid in the eluent fluid line at a first pressure and allow gas to flow through the separator to the wash fluid line at a second pressure lower than the first pressure. A method of using the high-pressure capillary degas assembly is also disclosed.

Abstract: A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel. The pressurized fluid channel may extend along an outer periphery of the low pressure fluid channel. The eluent may be received from an eluent generator at a pressure of at least about 3300 psi, and in various embodiments up to about 5000 psi. A liquid chromatography system and method are also disclosed.