Abstract: Described is a method of modulating neuronal network activities, the method including applying an alternating electric field (EF) to a neuronal network of neuronal cells in culture for a period of time, and increasing or decreasing a frequency of the alternating EF after the period of time to provide the neuronal network activities that are synchronizing or desynchronizing the neuronal network of neuronal cells in the culture, wherein increasing the frequency of the alternating electric field provides synchronizing neuronal network activities, and wherein decreasing the frequency of the alternating electric field provides desynchronizing neuronal network activities.

Abstract: The present disclosure relates generally to models and methods to modulate neuronal network activities simulating normal brain functions or neurological disorders in culture by applying alternating electric field. The electric field has alternating polarity and changing frequencies to achieve neuronal synchrony. The present disclosure also provides method of modulating modulate directed growth of neuronal axon by applying an alternating field electrical signal. The present disclosure also provides methods of screening compounds using the disclosed models, kits and systems thereof.

Abstract: Provided herein, in some embodiments, are human patient-derived brain tumor models and methods of use.

Abstract: Provided herein, in some embodiments, are methods comprising culturing human brain tissue spheroids on a three-dimensional scaffold in culture media comprising vascular endothelial growth factor (VEGF), and producing a population of cells, wherein cells of the population are positive for nestin, glial fibrillary acidic protein (GFAP), and/or vimentin.

Abstract: The present disclosure relates generally to models and methods to modulate neuronal network activities simulating normal brain functions or neurological disorders in culture by applying alternating electric field. The electric filed has alternating polarity and changing frequencies to achieve neuronal synchrony. The present disclosure also provides method of modulating modulate directed growth of neuronal axon by applying an alternating field electrical signal. The present disclosure also provides methods of screening compounds using the disclosed models, kits and systems thereof.

Abstract: Described herein are systems, methods and computer program products for real time assessment and monitoring of biological cells or their environment. In the systems and methods, a computer receives readouts of the biological cells or their environment and assesses changes in the biological cells or their environment by comparing current and previous values of parameters that describe the biological cells or their environment. The computer then determines treatments to be applies to the cells based on the assessing and user-specified criteria and then applies the treatment by controlling a setting on an environmental control unit to cause the treatment to be applied to the biological cells. The systems and methods described herein provide an automated system that includes computer-driven modifications that eliminate the need for the researcher to be present and controlling the flow of the experiment as it is being run.