Abstract: A method for performing gene editing on a target site in a cell, specifically a method for performing gene editing on a target site of a cell genome, comprising: (a) providing a cell to be genetically edited; (b) introducing into the cell (i) a gene editing enzyme or the encoding nucleic acid thereof or a first expression vector expressing the gene editing enzyme; and (ii) gRNA or a second expression vector expressing the gRNA, and performing gene editing on a target site of the cell genome, the gRNA directing the gene editing enzyme to perform fixed-point cutting on the target site; the targeting sequence of the gRNA targeting comprises one or more of the sequences shown in SEQ ID. No. 1-9. The method enables efficient gene editing to be performed on the target site.

Abstract: An organ perfusion solution includes a colloid component, a salt mixture, a buffer system, and a glutamine compound in a physiologically acceptable medium.

Abstract: Provided are a polymorph of an EP4 receptor antagonist, a preparation method therefor and a use thereof, relating in particular to a polymorph of an EP4 receptor antagonist (S)-4-(1-(2-(4-fluorobenzyl)-4, 7-dihydro-5H-thieno [2,3-c]pyran-3-carboxamido) ethyl) benzoic acid, a preparation method therefor and a use thereof.

Abstract: Provided are a fusion protein that improves gene editing efficiency and an application thereof. The fusion protein comprises a single-stranded DNA binding protein functional domain, nucleoside deaminase and nuclease. According to CBEs, when carrying our base conversion from C-G to T-A, nucleoside deaminase such as cytosine deaminase carries out deamination by using single-stranded DNA as a substrate, and by re-fusing the single-stranded DNA binding protein functional domain on the fusion protein of the nucleoside deaminase and nuclease, the chance of single-stranded DNA being exposed to the nucleoside deaminase is greatly increased, thereby significantly improving base editing efficiency. The present disclosure provides a breakthrough improvement of single-base gene editing technology and can greatly promote the application thereof in aspects such as gene editing, gene therapy, cell therapy, animal model making, and crop genetic breeding.

Abstract: Disclosed is an sgRNA guiding a PD1 gene for cleavage to achieve the efficient integration of exogenous sequences. The method for gene editing a PD1 gene in cells includes the steps of introducing a nuclease and an sgRNA into cells, and gene-editing the PD1 gene. The sgRNA guides the nuclease to cleave the PD1 gene and forms a broken site, at which an exogenous donor repair template can also be introduced, so that CAR-T elements can be directionally inserted at the specific site of the PD1 locus to construct enhanced CD19-CART cells with PD1 knockout in one step.

Abstract: Methods and kits for screening, diagnosing, detecting or predicting a patient outcome/risk variable for a lung transplant recipient after transplant or an EVLP outcome by measuring biomarker levels of at least three biomarkers selected from IL-6, IL-8, IL-10 and IL-1? optionally in combination with one or both of sTNFR1 and sTREM1 in EVLP perfusate are described. The methods involve for example, i. obtaining one or more test EVLP perfusate samples of a donor lung; ii. determining in one or more test EVLP perfusate sample of a donor lung, a polypeptide level of the at least three biomarkers selected from IL-8, IL-6, IL-10 and IL-1? and optionally one or both of sTNFR1 and sTREM1 i; and iii.

Abstract: Disclosed is a class of triaromatic compounds targeting a bifunctional phosphorylation site of STAT3, such as compounds represented by formulas (I) to (V), or pharmaceutically acceptable salts, hydrates, solvates, metabolites or prodrugs thereof. Further proposed are pharmaceutical compositions comprising the compounds represented by formulas (I) to (V). Further proposed are applications of the triaromatic compounds in the preparation of drugs for preventing and/or treating diseases caused or regulated by activation of the bifunctional phosphorylation site of STAT3.

Abstract: The present invention relates to a fusion protein for enhancing gene editing and use thereof. In particular, the invention provides an enhanced fusion protein. The enhanced fusion proteins of the present invention can significantly increase gene editing efficiency in vivo or in vitro as compared to the wildtype gene editing protein.

Abstract: A washing machine is provided with a control panel and is further provided with a connecting part of an intelligent terminal. The connecting part is in data connection with the intelligent terminal and is used for controlling the washing machine by the intelligent terminal. The control panel is of a panel structure which is not provided with a washing program control button, a display screen as well as a peripheral circuit for driving the display screen and the washing program control button. The washing machine is simple and generous in appearance, easy to operate, capable of greatly reducing the production cost and also saving manpower and material resources, and convenient to popularize.

Abstract: Disclosed by the present invention are a thienocyclic compound represented by formula (I), a pharmaceutically acceptable salt or a hydrate thereof, a composition containing the thienocyclic compound, and a preparation method therefor.

Abstract: An Internet of Things washing machine control method comprises the steps of, when a power module of the washing machine detects a startup signal, starting up the washing machine, and enabling a communication module to start to connect with the Internet; judging whether the communication module is successfully connected with the Internet, if yes, activating a remote control function and entering the next step, and if no, directly entering the next step; judging whether the washing machine receives an operating command of running a clothes washing program, if yes, executing the operating command and entering the next step, and if no, directly entering the next step; judging whether to shut down the washing machine, if yes, entering the next step, and if no, returning to the step; sending a shutdown signal to the power module, shutting down the washing machine, and powering off the communication module.

Abstract: Provided are two split single-base gene editing systems. Intein-mediated split BE3, saKKH-BE3 and ABE7.10 are separately developed by using the existing protein structure information of spCas9 and saCas9 and splitting methods thereof, and have targeted gene mutation efficiency equivalent to that of unsplit BE3, saKKH-BE3 and ABE7.10 working system, thereby making possible package into an AAV for delivery.

Abstract: Methods and kits for screening, diagnosing, detecting or predicting a patient outcome/risk variable for a lung transplant recipient after transplant or an EVLP outcome by measuring biomarker levels of one or more biomarkers selected from IL-6, IL-8, sTNFR1 and sTREM-1 in EVLP perfusate are described. The methods involve for example, i. obtaining one or more test EVLP perfusate samples of a donor lung; ii. determining in one or more test EVLP perfusate sample of a donor lung, a polypeptide level of one or more biomarkers selected from IL-8, IL-6, sTNFR1 and sTREM-1; and iii. a) comparing the polypeptide level of the one or more biomarkers in the perfusate sample with a control or cut-off level, wherein the differential level is indicative of outcome/risk of after transplant or of an EVLP outcome; or b) using the polypeptide level of one or several of the one or more biomarkers in combination, as part of an algebraic calculation of outcome/risk.

Abstract: The present disclosure discloses a washing method of a washing machine. The washing method comprises the following steps: a, identifying information of the laundry tags when laundries with laundry tags are placed into the washing machine, and screening the laundries according to the acquired information of the laundry tags; b, starting the washing machine, and determining a washing program according to the acquired information of the laundry tags in the step a; and c, identifying the information of the laundry tags again before water inflow of the washing machine, and screening the laundries and determining the washing program according to the acquired information of the laundry tags in the step c. By using the washing method of the washing machine, the laundry identification rate of the washing machine can be increased, the misreading rate can be reduced, and the washed laundry washing effect can be improved.

Abstract: A pharmaceutical composition comprising an ailanthone (AIL) compound or a derivative thereof, for the treatment of diseases or conditions caused by, dependent from, or associated with excessive androgen receptor activity. Ailantone compounds have been discovered to be a potent inhibitor of both full-length AR (AR-FL) and constitutively-active truncated AR splice variants (AR-Vs), and are found to possess favorable drug-like properties such as good bioavailability, high solubility, lack of CYP inhibition, and low hepatotoxicity. Methods of treatment are also provided.

Abstract: An organ perfusion device includes an inlet for connection to the organ, and an outlet for connection to the organ. The device also includes a perfusion circuit including: a reservoir configured to hold a perfusion fluid; a waste receptacle, and a plurality of fluid conduits. The fluid conduits define: a delivery fluid path connecting the reservoir with the inlet; a return fluid path, independent of the delivery fluid path, connecting the reservoir with the outlet; and a waste fluid path connecting the reservoir with the waste receptacle. The device also includes a first flow control device configured to selectively prevent or allow fluid flow from the reservoir to the organ via the delivery fluid path; and a second flow control device configured to selectively prevent or allow fluid flow from the reservoir to the waste receptacle via the waste fluid path.

Abstract: Perfusion solutions comprising A1AT for the ex vivo perfusion of donor organs are provided to improve donor organ quality and repair damaged donor organs for transplantation. Methods of ex vivo perfusion of donor organs with A1AT-containing perfusion solutions under normothermic temperatures are also provided.

Abstract: An Internet of Things washing machine control method comprises the steps of, when a power module of the washing machine detects a startup signal, starting up the washing machine, and enabling a communication module to start to connect with the Internet; judging whether the communication module is successfully connected with the Internet, if yes, activating a remote control function and entering the next step, and if no, directly entering the next step; judging whether the washing machine receives an operating command of running a clothes washing program, if yes, executing the operating command and entering the next step, and if no, directly entering the next step; judging whether to shut down the washing machine, if yes, entering the next step, and if no, returning to the step; sending a shutdown signal to the power module, shutting down the washing machine, and powering off the communication module.

Abstract: A washing machine is provided with a control panel and is further provided with a connecting part of an intelligent terminal. The connecting part is in data connection with the intelligent terminal and is used for controlling the washing machine by the intelligent terminal. The control panel is of a panel structure which is not provided with a washing program control button, a display screen as well as a peripheral circuit for driving the display screen and the washing program control button. The washing machine is simple and generous in appearance, easy to operate, capable of greatly reducing the production cost and also saving manpower and material resources, and convenient to popularize.

Abstract: There is described herein a method for the electrochemical quantification of a protein analyte in sample, comprising: providing one or more electrode(s), each comprising at least one peptide attached to its surface, the peptide being the protein or a fragment thereof; contacting the sample and electrode with an antibody in the presence of a redox reporter, wherein the antibody is capable of binding to each of the protein analyte and the peptide on the electrode; measuring an electrochemical signal generated by the redox reporter when a potential is applied; quantifying the protein analyte by comparing the electrochemical signal generated with a control, wherein the electrochemical signal is indirectly proportional to the amount of protein analyte in the sample.