Patents by Inventor Miroslav Radman
Miroslav Radman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240041727Abstract: The invention concerns glycosylated bacterioruberins isolated from monoglycosylated bacterioruberins, diglycosylated bacterioruberins, triglycosylated bacterioruberins, tetraglycosylated bacterioruberins, pentaglycosylated bacterioruberins, hexaglycosylated bacterioruberins, heptaglycosylated bacterioruberins, octaglycosylated bacterioruberins, nonaglycosylated bacterioruberins, decaglycosylated bacterioruberins, undecaglycosylated bacterioruberins, and dodecaglycosylated bacterioruberins. In particular, the present invention concerns the purification or synthesis of glycosylated forms of the carotenoid bacterioruberin, as well as its applications, in particular in the fields of pharmaceuticals, dermocosmetics and nutraceuticals and biotechnology.Type: ApplicationFiled: December 16, 2021Publication date: February 8, 2024Inventors: Jean-Noel THOREL, Miroslav RADMAN
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Publication number: 20230375540Abstract: The process of aging and the development of age-related diseases are related to the emerging phenotypes of increasingly damaged and progressively malfunctioning proteomes. The present invention provides methods of preventing aging and age-related diseases in mammals by assessment of protein-specific oxidative damage. Methods of providing treatments that reduce intracellular reactive oxygen and/or nitrogen species, or protein-specific damage caused by reactive oxygen and/or nitrogen species, are disclosed. Furthermore, methods of screening for compounds that reduce intracellular reactive oxidative species, and/or molecules that prevent protein-specific damage by protecting the susceptible protein from such damage and therefore prevent or treat degenerative or age-related diseases, are also disclosed.Type: ApplicationFiled: November 3, 2022Publication date: November 23, 2023Inventors: Miroslav Radman, Anita Krisko
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Publication number: 20190227058Abstract: The process of aging and the development of age-related diseases are related to the emerging phenotypes of increasingly damaged and progressively malfunctioning proteomes. The present invention provides methods of preventing aging and age-related diseases in mammals by assessment of protein-specific oxidative damage. Methods of providing treatments that reduce intracellular reactive oxygen and/or nitrogen species, or protein-specific damage caused by reactive oxygen and/or nitrogen species, are disclosed. Furthermore, methods of screening for compounds that reduce intracellular reactive oxidative species, and/or molecules that prevent protein-specific damage by protecting the susceptible protein from such damage and therefore prevent or treat degenerative or age-related diseases, are also disclosed.Type: ApplicationFiled: April 1, 2019Publication date: July 25, 2019Inventors: Miroslav Radman, Anita Krisko
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Publication number: 20180217133Abstract: The process of aging and the development of age-related diseases are related to the emerging phenotypes of increasingly damaged and progressively malfunctioning proteomes. The present invention provides methods of preventing aging and age-related diseases in mammals by assessment of protein-specific oxidative damage. Methods of providing treatments that reduce intracellular reactive oxygen and/or nitrogen species, or protein-specific damage caused by reactive oxygen and/or nitrogen species, are disclosed. Furthermore, methods of screening for compounds that reduce intracellular reactive oxidative species, and/or molecules that prevent protein-specific damage by protecting the susceptible protein from such damage and therefore prevent or treat degenerative or age-related diseases, are also disclosed.Type: ApplicationFiled: September 8, 2017Publication date: August 2, 2018Inventors: Miroslav Radman, Anita Krisko
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Publication number: 20180216153Abstract: Vaccines and therapeutic proteins, including polyclonal and monoclonal antibodies, must be maximally pure and stable in their most active native form. This is a requirement for their maximal efficacy, specificity and stability as well as for precluding immune responses against erroneous or damaged moieties. Similar considerations hold for proteins used in diagnostics, industry and research. The most frequent source of damage to proteins produced in living cells is the diverse product of oxidative damage. Two main sources of protein oxidation are the level of reactive oxygen species (ROS) and even more importantly the intrinsic susceptibility of proteins to oxidative damage. Methods for avoiding oxidative protein damage are disclosed, including providing for (i) a decrease in intracellular ROS levels and (ii) an increase in the intrinsic resilience of proteins to oxidative damage.Type: ApplicationFiled: September 12, 2017Publication date: August 2, 2018Inventors: Miroslav Radman, Anita Krisko
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Patent number: 9193974Abstract: This invention relates to chromosomal engineering via DNA repair process. The process of the invention comprises the steps of: 1) submitting at least one source of biological activity, e.g. Deinococcus radiodurans, to radiation, desiccation and/or chemical treatment liable to damage the DNA, so as to substantially shatter its chromosomes into short fragments; 2) annealing complementary single strand tails extended by the synthesis templated on partially overlapping DNA fragments of said shattered chromosomes; 4) converting the resulting long linear DNA intermediates into intact circular chromosomes, by means of a RecA dependent homologous recombination; whereas at least one foreign source of genetic material, e.g. DNA, can be introduced during steps 2 and/or 3; and 4) optionally separating and collecting the recombined chromosomes thus obtained.Type: GrantFiled: May 10, 2006Date of Patent: November 24, 2015Assignee: DEINOVEInventors: Miroslav Radman, Ksenija Zahradka
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Publication number: 20140235735Abstract: The process of aging and the development of age-related diseases are related to the emerging phenotypes of increasingly damaged and progressively malfunctioning proteomes. The present invention provides methods of preventing aging and age-related diseases in mammals by assessment of protein-specific oxidative damage. Methods of providing treatments that reduce intracellular reactive oxygen and/or nitrogen species, or protein-specific damage caused by reactive oxygen and/or nitrogen species, are disclosed. Furthermore, methods of screening for compounds that reduce intracellular reactive oxidative species, and/or molecules that prevent protein-specific damage by protecting the susceptible protein from such damage and therefore prevent or treat degenerative or age-related diseases, are also disclosed.Type: ApplicationFiled: February 15, 2014Publication date: August 21, 2014Applicant: MEDITERRANEAN INSTITUTE FOR LIFE SCIENCESInventors: Miroslav Radman, Anita Krisko
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Publication number: 20140227741Abstract: Vaccines and therapeutic proteins, including polyclonal and monoclonal antibodies, must be maximally pure and stable in their most active native form. This is a requirement for their maximal efficacy, specificity and stability as well as for precluding immune responses against erroneous or damaged moieties. Similar considerations hold for proteins used in diagnostics, industry and research. The most frequent source of damage to proteins produced in living cells is the diverse product of oxidative damage. Two main sources of protein oxidation are the level of reactive oxygen species (ROS) and even more importantly the intrinsic susceptibility of proteins to oxidative damage. Methods for avoiding oxidative protein damage are disclosed, including providing for (i) a decrease in intracellular ROS levels and (ii) an increase in the intrinsic resilience of proteins to oxidative damage.Type: ApplicationFiled: December 18, 2013Publication date: August 14, 2014Applicant: Mediterranean Institute for Life SciencesInventors: Miroslav Radman, Anita Krisko
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Publication number: 20120184030Abstract: The present invention relates to methods for establishing a symbiosis, including the following steps: selecting an organism or an organelle to constitute the symbiont and an organism to constitute the host, the latter not existing naturally in a symbiotic relationship; contacting the symbiont and the host; and maintaining the combination of the symbiont and the host.Type: ApplicationFiled: July 19, 2010Publication date: July 19, 2012Inventors: Zoran Dermanovic, Miroslav Radman
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Patent number: 7883894Abstract: The present invention relates to a process for allowing homologous recombination between non-identical DNA sequences of an organism and various applications thereof.Type: GrantFiled: December 19, 2002Date of Patent: February 8, 2011Assignee: Mixis France S.A.Inventors: Ivan Matic, Miroslav Radman
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Publication number: 20100055669Abstract: In vivo methods for generating and detecting recombinant DNA sequences in bacteriophages or plasmids containing bacteriophage sequences, methods for generating hybrid genes and hybrid proteins encoded by these hybrid genes by the use of bacteriophages and plasmids containing bacteriophage sequences, bacteriophages and plasmids that can be used in these methods, and kits comprising appropriate bacterial host cells and bacteriophages or plasmids are described.Type: ApplicationFiled: July 6, 2005Publication date: March 4, 2010Applicant: MIXIS FRANCE, S.A.Inventors: Alejandro Luque, Heike Strobel, Jann Thorsten Martinsohn, Marie-Agnès Petit, Miroslav Radman
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Publication number: 20090227029Abstract: This invention relates to chromosomal engineering via DNA repair process. The process of the invention comprises the steps of: 1) submitting at least one source of biological activity, e.g. Deinococcus radiodurans, to radiation, desiccation and/or chemical treatment liable to damage the DNA, so as to substantially shatter its chromosomes into short fragments; 2) annealing complementary single strand tails extended by the synthesis templated on partially overlapping DNA fragments of said shattered chromosomes; 4) converting the resulting long linear DNA intermediates into intact circular chromosomes, by means of a RecA dependent homologous recombination; whereas at least one foreign source of genetic material, e.g. DNA, can be introduced during steps 2 and/or 3; and 4) optionally separating and collecting the recombined chromosomes thus obtained.Type: ApplicationFiled: May 10, 2006Publication date: September 10, 2009Inventors: Miroslav Radman, Ksenija Zahradka
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Publication number: 20070212692Abstract: The present invention relates in general to methods for generating and detecting recombinant DNA sequences in prokaryotic cells, in particular bacteria, by using two different extrachromosomal elements, in particular Saccharomyces cerevisiae and plasmids that can be used for conducting the inventive methods. DNA sequences for which these methods are relevant include protein-encoding and non-coding sequences.Type: ApplicationFiled: February 26, 2005Publication date: September 13, 2007Inventors: Ana Gomez-Rodriguez, Tatjana Galic, Marie-Agnes Petite, Ivan Matic, Miroslav Radman
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Publication number: 20050176149Abstract: The present invention relates to a process for allowing homologous recombination between non-identical DNA sequences of an organism and various applications thereof.Type: ApplicationFiled: December 19, 2002Publication date: August 11, 2005Inventors: Ivan Matic, Miroslav Radman
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Publication number: 20020132259Abstract: A method for detecting mutations and polymorphisms, including single nucleotide polymorphisms (SNP's), is based on the use of RecA-like recombinase protein and a MutS-like mismatch binding protein. RecA coated, specific DNA oligonucleotide probes (RecA filaments) are used for homology searching in duplex DNA. Location of homologous sequences results in the formation of D-loop structures containing a duplex region comprised of the oligonucleotide probe and one strand of the test DNA. Mismatches or unpaired bases in the duplex region are substrates for MutS binding. Co-localization of MutS and oligonucleotide or RecA labels is diagnostic of specific sequence differences between the probe and test DNAs. Also provided are compositions and kits useful for practicing the methods of the present invention.Type: ApplicationFiled: February 20, 2002Publication date: September 19, 2002Inventors: Robert E. Wagner, Miroslav Radman
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Patent number: 5965415Abstract: The present invention relates to a process of recombination in vivo of partially homologous DNA sequences having up to 30% of base mismatches. According to its essential characteristic, said sequences are placed together in cells or an organism of which the enzymatic mismatch repair system is defective or has been transitorily inactivated by saturation for the time to obtain recombination between said DNA sequences or in using mutants which increase the intergeneric recombination.Type: GrantFiled: June 7, 1995Date of Patent: October 12, 1999Assignee: Mixis France, S.A.Inventors: Miroslav Radman, Christiane Rayssiguier
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Patent number: 5912119Abstract: The present invention relates to a process of recombination in vivo of partially homologous DNA sequences having up to 30% of base mismatches. According to its essential characteristic, said sequences are placed together in cells or an organism of which the enzymatic mismatch repair system is defective or has been transitorily inactivated by saturation for the time to obtain recombination between said DNA sequences or in using mutants which increase the intergeneric recombination.Type: GrantFiled: April 25, 1994Date of Patent: June 15, 1999Assignee: Mixis France, S.A.Inventors: Miroslav Radman, Christiane Rayssiguier
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Patent number: 5334522Abstract: A method of producing plasmids with heteroduplex DNA sequences, in which probe plasmids containing DNA inserts that correspond to probe sequences and test plasmids containing DNA inserts that correspond to a population of test sequences are first constructed. The vector portions of the plasmids used in these constructions have similar sequences. When test and probe plasmids are cleaved with appropriate restriction enzymes and then denatured to separate strands, complementary regions of the linear strands which correspond to vector sequences can anneal. The molecules that harbor test and probe inserts that are related by sequence complementarity form non-covalently closed circular molecules. These molecules can replicate after transformation into an appropriate host organism. There is a replication bias against plasmids which anneal through vector sequences, but which do not contain homologous probe and test inserts.Type: GrantFiled: March 27, 1992Date of Patent: August 2, 1994Assignee: United States/National Institutes of HealthInventors: Michael A. Resnick, Miroslav Radman