Patents by Inventor Mitsufumi Wada
Mitsufumi Wada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11685704Abstract: A method of producing THB with improved efficiency is provided. Provided is a method of producing trihydroxybenzene (THB), the method comprising a step of heating a bacterial culture liquid comprising deoxy-scyllo-inosose (DOI) at a high temperature of no lower than 80° C. to obtain a product solution comprising trihydroxybenzene (THB).Type: GrantFiled: October 24, 2019Date of Patent: June 27, 2023Assignee: MITSUI CHEMICALS, INC.Inventors: Osamu Yoshikawa, Masaki Yabe, Mitsufumi Wada, Yusuke Ito, Hideki Ouchi
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Patent number: 11499175Abstract: A polypeptide includes, in the amino acid sequence of SEQ ID NO: 1 or a similar sequence, at least one specific amino acid substitution on at least one of the 14th, 37th, 209th, 293rd, and 319th amino acid residues from the N-terminal of the amino acid sequence of SEQ ID NO: 1. A polynucleotide, an expression cassette, a vector, and a transformant include a base sequence encoding the amino acid sequence of the polypeptide. A method of producing the polypeptide and a method of producing 2-deoxy-scyllo-inosose are also provided.Type: GrantFiled: March 15, 2018Date of Patent: November 15, 2022Assignee: MITSUI CHEMICALS, INC.Inventors: Hiroaki Takaku, Harutake Yamazaki, Mitsufumi Wada, Daisuke Miyazawa
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Publication number: 20210331993Abstract: A method of producing THB with improved efficiency is provided. Provided is a method of producing trihydroxybenzene (THB), the method comprising a step of heating a bacterial culture liquid comprising deoxy-scyllo-inosose (DOI) at a high temperature of no lower than 80° C. to obtain a product solution comprising trihydroxybenzene (THB).Type: ApplicationFiled: October 24, 2019Publication date: October 28, 2021Applicant: MITSUI CHEMICALS, INC.Inventors: Osamu YOSHIKAWA, Masaki YABE, Mitsufumi WADA, Yusuke ITO, Hideki OUCHI
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Publication number: 20210002687Abstract: A polypeptide includes, in the amino acid sequence of SEQ ID NO: 1 or a similar sequence, at least one specific amino acid substitution on at least one of the 14th, 37th, 209th, 293rd, and 319th amino acid residues from the N-terminal of the amino acid sequence of SEQ ID NO: 1. A polynucleotide, an expression cassette, a vector, and a transformant include a base sequence encoding the amino acid sequence of the polypeptide. A method of producing the polypeptide and a method of producing 2-deoxy-scyllo-inosose are also provided.Type: ApplicationFiled: March 15, 2018Publication date: January 7, 2021Applicants: THE NIIGATA INSTITUTE OF SCIENCE AND TECHNOLOGY, MITSUI CHEMICALS, INC.Inventors: Hiroaki TAKAKU, Harutake YAMAZAKI, Mitsufumi WADA, Daisuke MIYAZAWA
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Patent number: 9822387Abstract: An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.Type: GrantFiled: July 27, 2012Date of Patent: November 21, 2017Assignee: MITSUI CHEMICALS, INC.Inventors: Ryota Fujii, Tomokazu Shirai, Tadashi Araki, Koh Amano, Yoshiko Matsumoto, Toshihiro Tateno, Nozomi Takebayashi, Takashi Morishige, Hitoshi Takahashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Tomonori Hidesaki, Ayako Endo, Dominik Lukas Jürgen-Lohmann, Anjali Madhavan, Su Sun Chong
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Patent number: 9267156Abstract: An isopropyl alcohol-producing Escherichia coli includes an isopropyl alcohol production system, wherein an activity of transcriptional repressor GntR is inactivated, and the isopropyl alcohol-producing Escherichia coli preferably further includes a group of auxiliary enzymes having an enzyme activity expression pattern with which isopropyl alcohol production capacity achieved by the inactivation of the GntR activity is maintained or enhanced. A method of producing isopropyl alcohol includes producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli. A method of producing acetone includes contacting the isopropyl alcohol obtained by the isopropyl alcohol production method with a complex oxide that includes zinc oxide and at least one oxide containing a Group 4 element, and that is prepared by coprecipitation.Type: GrantFiled: August 11, 2011Date of Patent: February 23, 2016Assignee: MITSUI CHEMICALS, INC.Inventors: Koh Amano, Tomokazu Shirai, Hitoshi Takahashi, Junichiro Hirano, Yoshiko Matsumoto, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Patent number: 9150868Abstract: Disclosed is an Escherichia coli producing 2-deoxy-scyllo-inosose (DOI), which, from a sucrose non-PTS gene group, has at least a sucrose hydrolase (CscA)-encoding gene and which is provided with a DOI production system or has an enhanced DOI production system. The Escherichia coli preferably further includes a system to enhance sugar uptake capacity. There is also disclosed a method of producing DOI from a plant-derived raw material containing sucrose by using the Escherichia coli.Type: GrantFiled: October 30, 2009Date of Patent: October 6, 2015Assignee: MITSUI CHEMICALS, INC.Inventors: Junko Tokuda, Tomoyuki Natsuji, Hitoshi Takahashi, Tadashi Araki, Takashi Morishige, Katsuyuki Takahashi, Mitsufumi Wada
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Patent number: 9133444Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.Type: GrantFiled: September 14, 2012Date of Patent: September 15, 2015Assignee: MITSUI CHEMICALS, INC.Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
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Patent number: 9096842Abstract: The present invention provides an isopropyl alcohol-producing Escherichia coli that includes at least a sucrose hydrolase gene that belongs to a sucrose non-PTS gene group, and an imparted or enhanced isopropyl alcohol production system, and an isopropyl alcohol production method of producing isopropyl alcohol from a sucrose-containing plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.Type: GrantFiled: September 13, 2010Date of Patent: August 4, 2015Assignee: MITSUI CHEMICALS, INC.Inventors: Takashi Morishige, Hitoshi Takahashi, Nozomi Takebayashi, Mitsufumi Wada
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Patent number: 9074226Abstract: The invention provides: an isopropyl alcohol-producing bacterium which has an acetoacetate decarboxylase activity, an isopropyl alcohol dehydrogenase activity, a CoA transferase activity and a thiolase activity having been imparted thereto and is capable of producing isopropyl alcohol from a plant-derived material; a method of producing isopropyl alcohol whereby isopropyl alcohol is produced from a plant-derived material by using this isopropyl alcohol-producing bacterium; and an apparatus therefor.Type: GrantFiled: July 4, 2008Date of Patent: July 7, 2015Assignee: MITSUI CHEMICALS, INC.Inventors: Nozomi Takebayashi, Mitsufumi Wada, Daisuke Mochizuki, Fuminobu Yoshimi, Seiichi Watanabe, Hitoshi Takahashi, Takashi Morishige
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Patent number: 8932845Abstract: An isopropyl alcohol-producing Escherichia coli equipped with an isopropyl alcohol production system, having at least one enhanced enzyme activity selected from the group consisting of an enhanced malate dehydrogenase activity, an enhanced NAD(P)+ transhydrogenase (AB-specific) activity, and an enhanced thiolase activity, and an isopropyl alcohol producing method including producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.Type: GrantFiled: March 4, 2011Date of Patent: January 13, 2015Assignee: Mitsui Chemicals, Inc.Inventors: Yoshiko Matsumoto, Junichiro Hirano, Takashi Morishige, Tomokazu Shirai, Hitoshi Takahashi, Koh Amano, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Publication number: 20140363847Abstract: An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.Type: ApplicationFiled: July 27, 2012Publication date: December 11, 2014Inventors: Ryota Fujii, Tomokazu Shirai, Tadashi Araki, Koh Amano, Yoshiko Matsumoto, Toshihiro Tateno, Nozomi Takebayashi, Takashi Morishige, Hitoshi Takahashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Tomonori Hidesaki, Ayako Endo, Dominik Lukas Jürgen-Lohmann, Anjali Madhavan
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Patent number: 8748157Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism.Type: GrantFiled: April 27, 2007Date of Patent: June 10, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
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Patent number: 8728780Abstract: An object of the invention is to produce a microorganism which yields high amounts in a short time of a hydroxycarboxylic acid reduced in impurity content. This invention further provides a process for producing a hydroxycarboxylic acid, including glycolic acid, using the microorganism. This process enables a hydroxycarboxylic acid having high purity to be supplied at low cost.Type: GrantFiled: April 19, 2005Date of Patent: May 20, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Mitsufumi Wada, Daisuke Mochizuki, Hitoshi Takahashi, Takashi Morishige, Katsuyuki Takahashi
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Patent number: 8679800Abstract: The present invention provides: a lactic acid-producing Escherichia coli comprising at least one gene of a sucrose non-PTS gene group, including at least a sucrose hydrolase gene, provided that a combination of a repressor protein (cscR), a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) and a combination of a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) are excluded, wherein the lactic acid-producing Escherichia coli comprises a lactic acid production enhancing system provided by genetic recombination; and a lactic acid production method including producing lactic acid from a plant-derived sucrose-containing raw material by using the lactic acid-producing Escherichia coli.Type: GrantFiled: September 11, 2009Date of Patent: March 25, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Takashi Morishige, Katsuyuki Takahashi, Hitoshi Takahashi, Mitsufumi Wada, Daisuke Mochizuki, Daisuke Miyazawa, Tadashi Araki
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Patent number: 8614076Abstract: The present invention provides: a lactic acid-producing Escherichia coli including an enzymatic activity of at least one NAD-dependent lactate dehydrogenase and an enzymatic activity of at least one NAD-independent lactate oxidoreductase, both of which are enhanced so as to decompose one of D-lactic acid or L-lactic acid and to produce the other one of D-lactic acid or L-lactic acid; and a lactic acid production method using the lactic acid-producing Escherichia coli.Type: GrantFiled: September 11, 2009Date of Patent: December 24, 2013Assignee: Mitsui Chemicals, Inc.Inventors: Mitsufumi Wada, Katsuyuki Takahashi, Takashi Morishige, Daisuke Miyazawa, Hitoshi Takahashi, Daisuke Mochizuki, Tadashi Araki
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Publication number: 20130211170Abstract: An isopropyl alcohol-producing Escherichia coli includes an isopropyl alcohol production system, wherein an activity of transcriptional repressor GntR is inactivated, and the isopropyl alcohol-producing Escherichia coli preferably further includes a group of auxiliary enzymes having an enzyme activity expression pattern with which isopropyl alcohol production capacity achieved by the inactivation of the GntR activity is maintained or enhanced. A method of producing isopropyl alcohol includes producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli. A method of producing acetone includes contacting the isopropyl alcohol obtained by the isopropyl alcohol production method with a complex oxide that includes zinc oxide and at least one oxide containing a Group 4 element, and that is prepared by coprecipitation.Type: ApplicationFiled: August 11, 2011Publication date: August 15, 2013Inventors: Koh Amano, Tomokazu Shirai, Hitoshi Takahashi, Junichiro Hirano, Yoshiko Matsumoto, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Publication number: 20130029392Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.Type: ApplicationFiled: September 14, 2012Publication date: January 31, 2013Applicant: MITSUI CHEMICALS, INC.Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
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Publication number: 20130005008Abstract: An isopropyl alcohol-producing Escherichia coli equipped with an isopropyl alcohol production system, having at least one enhanced enzyme activity selected from the group consisting of an enhanced malate dehydrogenase activity, an enhanced NAD(P)+ transhydrogenase (AB-specific) activity, and an enhanced thiolase activity, and an isopropyl alcohol producing method including producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.Type: ApplicationFiled: March 4, 2011Publication date: January 3, 2013Inventors: Yoshiko Matsumoto, Junichiro Hirano, Takashi Morishige, Tomokazu Shirai, Hitoshi Takahashi, Koh Amano, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Publication number: 20120237992Abstract: The present invention provides an isopropyl alcohol-producing Escherichia coli that includes at least a sucrose hydrolase gene that belongs to a sucrose non-PTS gene group, and an imparted or enhanced isopropyl alcohol production system, and an isopropyl alcohol production method of producing isopropyl alcohol from a sucrose-containing plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.Type: ApplicationFiled: September 13, 2010Publication date: September 20, 2012Inventors: Takashi Morishige, Hitoshi Takahashi, Nozomi Takebayashi, Mitsufumi Wada