Abstract: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can fuse to the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Abstract: The disclosure provides, for example, single stranded, covalently closed DNA that does not form a double stranded structure longer than 100 base pairs. The ssDNA may encode an effector sequence, for instance a therapeutic protein. The ssDNA may comprise a nuclear targeting sequence (NTS). In some embodiments, the ssDNA shows decreased activation of the innate immune system compared to an otherwise similar dsDNA.

Abstract: Accurate function estimations and well-calibrated uncertainties are important for Bayesian optimization (BO). Most theoretical guarantees for BO are established for methods that model the objective function with a surrogate drawn from a Gaussian process (GP) prior. GP priors are poorly-suited for discrete, high-dimensional, combinatorial spaces, such as biopolymer sequences. Using a neural network (NN) as the surrogate function can obtain more accurate function estimates. Using a NN can allow arbitrarily complex models, removing the GP prior assumption, and enable easy pretraining, which is beneficial in the low-data BO regime. However, a fully-Bayesian treatment of uncertainty in NNs remains intractable, and existing approximate methods, like Monte Carlo dropout and variational inference, can highly miscalibrate uncertainty estimates.

Abstract: Described herein are methods of reducing, preventing, or reducing the risk of, an adverse effect of a pathogen. In some embodiments, the pathogen is a drug resistant pathogen.

Abstract: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Abstract: Methods and compositions for modulating a target genome are disclosed.

Abstract: Systems, apparatuses, software, and methods for engineering amino acid sequences configured to have specific protein functions or properties. Machine learning is implemented by methods to process an input seed sequence and generate as output an optimized sequence having the desired function or property.

Abstract: Compositions, e.g., pharmaceutical compositions, nutritional compositions, medical foods, and food ingredients, as well as their methods of use, are provided, for treating diseases associated with hyperammonemia, e.g., urea cycle disorders (UCD) and hepatic encephalopathy (HE), e.g., minimal HE (MHE) and overt HE (OHE), and modulating enzyme activities and levels of microbes and taxa, and metabolites (e.g., ammonia) in the microbiome of a subject.

Abstract: Systems, apparatuses, software, and methods for identifying associations between amino acid sequences and protein functions or properties. The application of machine learning is used to generate models that identify such associations based on input data such as amino acid sequence information. Various techniques including transfer learning can be utilized to enhance the accuracy of the associations.

Abstract: This disclosure provides, e.g., cytobiologic compositions and methods of use thereof. The cytobiologics can be used, e.g., to deliver a protein or nucleic acid to a target cell.

Abstract: Compositions of glycan polymers and methods of making and manufacturing the same are described herein. Also provided are methods of treating a disease or disorder with a glycan polymer preparation.

Abstract: Described herein are methods of reducing, preventing, or reducing the risk of, an adverse effect of a pathogen using a glycan preparation. In some embodiments, the pathogen is a drug resistant pathogen.

Abstract: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Abstract: Compositions of glycan polymers and methods of making and manufacturing the same are described herein. Also provided are methods of treating a disease or disorder with a glycan polymer preparation.