Patents by Inventor Moo-Yeal Lee
Moo-Yeal Lee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11773357Abstract: A perfusion plate that can be combined with pillar plates containing cell layers is disclosed. The perfusion plate can have an inflow reservoir and an outflow reservoir connected by at least one channel, which fluidly connects the perfusion wells to the reservoirs for the flow of a fluid such as growth media. A perfusion plate can be part of an assembly containing a pillar plate, a lid, and a transparent bottom for visualizing cell growth in the perfusion wells. The perfusion-pillar plate assembly can facilitate perfusion-based tissue culture and tissue communication for high throughput, high-content, drug screening.Type: GrantFiled: October 23, 2020Date of Patent: October 3, 2023Assignee: Cleveland State UniversityInventor: Moo-Yeal Lee
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Patent number: 11390836Abstract: A micropillar and microwell chip and methods of studying cellular environments using micropillar and microwell chips is disclosed. The micropillar chip may include at least one micropillar with a pillar-microwell. The microwell chip may include at least one microwell with an upper and a lower microwell. A perfusion channel chip that may be integrated with a micropillar chip is also disclosed. The perfusion channel chip may include a channel, a pillar-insertion hole, a membrane cassette, and a reservoir well.Type: GrantFiled: November 17, 2017Date of Patent: July 19, 2022Assignee: Cleveland State UniversityInventor: Moo-Yeal Lee
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Patent number: 11262349Abstract: A multiplexed method of monitoring immune-cell responses to different ligands using a micropillar/microwell plate platform is disclosed. The method may include dispensing immune cells onto at least one micropillar chip and inserting the at least one micropillar into at least one microwell on a microwell chip, in which the at least one microwell contains at least one test compound. The method may also include immobilizing antibodies onto at least one micropillar on a micropillar chip and inserting the at least one micropillar into at least one microwell on a microwell chip, in which the at least one microwell contains a test compound. The method may also include treating the micropillar chip with at least one reactive polymer.Type: GrantFiled: October 11, 2018Date of Patent: March 1, 2022Assignee: Cleveland State UniversityInventors: Moo-Yeal Lee, Xue-Long Sun
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Publication number: 20210123007Abstract: A perfusion plate that can be combined with pillar plates containing cell layers is disclosed. The perfusion plate can have an inflow reservoir and an outflow reservoir connected by at least one channel, which fluidly connects the perfusion wells to the reservoirs for the flow of a fluid such as growth media. A perfusion plate can be part of an assembly containing a pillar plate, a lid, and a transparent bottom for visualizing cell growth in the perfusion wells. The perfusion-pillar plate assembly can facilitate perfusion-based tissue culture and tissue communication for high throughput, high-content, drug screening.Type: ApplicationFiled: October 23, 2020Publication date: April 29, 2021Applicant: Cleveland State UniversityInventor: Moo-Yeal Lee
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Patent number: 10934538Abstract: A method of creating a miniature multicellular biological construct and a method for studying cellular environments using the miniature multicellular biological construct is provided. The method for making the miniature multicellular biological construct includes suspending cells in a hydrogel, depositing the cell-suspension into a microwell, gelling the cell-suspension, and incubating the cell-suspension. The method for studying cellular environments includes imaging the miniature multicellular biological construct.Type: GrantFiled: January 12, 2017Date of Patent: March 2, 2021Assignee: Cleveland State UniversityInventor: Moo-Yeal Lee
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Publication number: 20190107532Abstract: A multiplexed method of monitoring immune-cell responses to different ligands using a micropillar/microwell plate platform is disclosed. The method may include dispensing immune cells onto at least one micropillar chip and inserting the at least one micropillar into at least one microwell on a microwell chip, in which the at least one microwell contains at least one test compound. The method may also include immobilizing antibodies onto at least one micropillar on a micropillar chip and inserting the at least one micropillar into at least one microwell on a microwell chip, in which the at least one microwell contains a test compound. The method may also include treating the micropillar chip with at least one reactive polymer.Type: ApplicationFiled: October 11, 2018Publication date: April 11, 2019Applicant: Cleveland State UniversityInventors: Moo-Yeal Lee, Xue-Long Sun
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Publication number: 20180142195Abstract: A micropillar and microwell chip and methods of studying cellular environments using micropillar and microwell chips is disclosed. The micropillar chip may include at least one micropillar with a pillar-microwell. The microwell chip may include at least one microwell with an upper and a lower microwell. A perfusion channel chip that may be integrated with a micropillar chip is also disclosed. The perfusion channel chip may include a channel, a pillar-insertion hole, a membrane cassette, and a reservoir well.Type: ApplicationFiled: November 17, 2017Publication date: May 24, 2018Inventor: Moo-Yeal Lee
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Publication number: 20170198275Abstract: A method of creating a miniature multicellular biological construct and a method for studying cellular environments using the miniature multicellular biological construct is provided. The method for making the miniature multicellular biological construct includes suspending cells in a hydrogel, depositing the cell-suspension into a microwell, gelling the cell-suspension, and incubating the cell-suspension. The method for studying cellular environments includes imaging the miniature multicellular biological construct.Type: ApplicationFiled: January 12, 2017Publication date: July 13, 2017Inventor: Moo-Yeal Lee
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Publication number: 20140273053Abstract: Disclosed is a cell chip and methods for the use thereof, wherein the cell chip includes a substrate made of an opaque material and having a plurality of insertion holes formed therein, a filler made of a transparent material and inserted into each of the insertion holes so as to protrude from the substrate, and a biomatrix which is formed on the filler and immobilizes a biomaterial. Also, another substrate having a plurality of wells which store a fluid is further provided thus forming a 3D cell chip.Type: ApplicationFiled: February 12, 2014Publication date: September 18, 2014Applicants: Samsung Electro-Mechanics Co. Ltd., Solidus BioSciences, Inc.Inventors: Moo-Yeal Lee, Se Hoon Jeong, Bo Sung Ku
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Publication number: 20140227145Abstract: There is provided a fluid discharging device including: a first pressure generating unit generating a first pressure for discharging a fluid; a second pressure generating unit generating a second pressure for discharging a fluid, and being controllable so as to change a magnitude of the second pressure; and a nozzle discharging the fluid pressurized by the first and second pressure generating units.Type: ApplicationFiled: May 17, 2012Publication date: August 14, 2014Applicants: SAMSUNG ELECTRO-MECHANICS, INC., SOLIDUS BIOSCIENCES, INC.Inventors: Sang Jin Kim, Suk-ho Song, Bo Sung Ku, Moo-Yeal Lee
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Publication number: 20140045256Abstract: There is provided a cell chip including: a first substrate having biomaterials fixed thereto; a second substrate provided with one or more receiving space in which a culture medium is stored; and a circulation unit circulating the culture medium stored in the receiving space.Type: ApplicationFiled: July 3, 2013Publication date: February 13, 2014Inventors: Dong Woo LEE, Bo Sung KU, Sang Jin KIM, Moo-Yeal LEE
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Publication number: 20120165224Abstract: Disclosed herein is a cell chip including: an upper substrate having a plurality of biometrics spaced therefrom by fillers, the biometrics fixing a biomaterial thereinto; a first hydrophobic coating layer formed on the upper substrate; and a lower substrate combined with the upper substrate and having a plurality of wells formed therein, the well storing fluid provided to the biometrics therein. The cell chip includes two substrates functionally separated from each other to implement a three dimensional cell chip, solves a cross contamination problem, and provides a similar environment to a bio environment, thereby making it possible to increase the accuracy of a test.Type: ApplicationFiled: December 22, 2011Publication date: June 28, 2012Inventors: Suk Ho SONG, Bo Sung Ku, Se Hoon Jeong, Moo Yeal Lee, Dong Woo Lee
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Publication number: 20120088693Abstract: Disclosed herein is a microarray cell chip. The microarray cell chip includes an upper substrate that has biomatrices encapsulating biomaterials formed on one surface thereof and through holes penetrating from one surface to the other surface thereof and a lower substrate that is coupled with the upper substrate and is provided with wells storing reagents supplied to the biomatrices. The microarray cell chip according to the present invention can smoothly transfer the culture media and the reagents to the biomaterials embedded in the biomatrices through the diffusion and simply separate the upper substrate from the lower substrate, thereby improving the easiness of washing. Therefore, the present invention provides an environment similar to the bio environment, thereby making it possible to increase accuracy of an experiment.Type: ApplicationFiled: September 9, 2011Publication date: April 12, 2012Inventors: Moo-Yeal Lee, Dong Woo Lee, Se Hoon Jeong, Jeong Suong Yang, Bo Sung Ku
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Publication number: 20110190162Abstract: The invention is directed to a three-dimensional cell culture array comprising spatially-separated matrices attached to a solid support, wherein a plurality of said matrices encapsulate cells transfected with nucleic acids, method for the preparation of the array and methods reducing the expression of a target gene.Type: ApplicationFiled: November 12, 2010Publication date: August 4, 2011Inventors: Moo-Yeal Lee, Seok Joon Kwon, Jonathan S. Dordick, Douglas S. Clark, Jessica R. McKinley
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Publication number: 20090221441Abstract: The present invention is directed to a screening platform employing a miniaturized three-dimensional cell chip for high-throughput toxicology screening of test and lead compounds, prodrugs, drugs and P-450 generated drug metabolites. To this end, the three-dimensional cell chip, employs human cells encapsulated in a matrix (e.g., collagen or alginate gels) in volumes as small as 10 nL arrayed on a functionalized substrates (e.g., glass microscope slides) for spatially addressable screening against multiple test compounds. With the present platform, over 3,000 cell-matrix islands may be spotted providing for simultaneous screening against multiple compounds at multiple doses and in high replicate.Type: ApplicationFiled: October 31, 2006Publication date: September 3, 2009Applicants: Regents of the University of CaliforniaInventors: Moo-Yeal Lee, Anand K. Ramasubramanian, Douglas S. Clark, Jonathan S. Dordick
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Publication number: 20070108057Abstract: Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.Type: ApplicationFiled: January 9, 2007Publication date: May 17, 2007Inventors: Jonathan Dordick, Moo-Yeal Lee, Aravind Srinivasan, Bosung Ku
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Patent number: 7172682Abstract: Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.Type: GrantFiled: January 24, 2003Date of Patent: February 6, 2007Assignee: Rensselaer Polytechnic InstituteInventors: Jonathan S. Dordick, Moo-Yeal Lee, Aravind Srinivasan, Bosung Ku
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Publication number: 20070000781Abstract: Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst- or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.Type: ApplicationFiled: January 24, 2003Publication date: January 4, 2007Applicant: Rensselaer Polytechnic InstituteInventors: Jonathan Dordick, Moo-Yeal Lee, Aravind Srinivasan, Bosung Ku