Abstract: Natural product metabolites produced by fungi are synthesized using fungal spores that have been immobilized onto/into a support. Supports that can be used include loofah sponge, synthetic sponge, powdered cellulose paper, wood shavings, calcium alginate gel beads, agar gel beads, channeled aluminum beads, polypropylene beads and glass beads. Immobilizing the fungal spores provides accelerated production of the natural product metabolite in a standard bioreactor. Fungi that can be used include Penicillium cyclopium, Penicillium chrysogenum, Penicillium citrinum, Trichoderma viride, Aspergillus terreus and Monascus ruber. In a preferred embodiment, spores of Penicillium cyclopium NRRL 6233 immobilized with loofah sponge are cultured in a nutrient media for at least 4 days to produce compactin which is a hypocholesteremic agent.

Abstract: A method for the accelerated brewing of alcohol and alcoholic beverages including beer, wine, and spirits utilizing flavor producing strains of S. cerevisiae and/or other bacteria such as lactobacillus spp. and a high alcohol-tolerant strain of S. cerevisiae. Preferred strains of S. cerevisiae for the production of beer are ATCC Nos. 20866 and 20867.In the preferred method, the fermentation is conducted in a bioreactor containing the flavor-producing strains and the high alcohol tolerant strain of S. cerevisiae immobilized on inert, high surface area supports, preferably porous organic or glass beads. The bioreactor significantly decreases the fermentation time necessary to produce a beverage containing a desired alcohol percentage. For example, beer can be processed in less than about 12 to 18 hours, wine in less than about 36 hours.