Patents by Inventor Motoo Watanabe
Motoo Watanabe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240000050Abstract: The present invention provides a method for preparing a somatic chimera by using a pluripotent cell genetically engineered not to differentiate into a predetermined type of cell. The present invention particularly provides a method for preventing a pluripotent cell from contributing to the brain and gonad in a somatic chimera animal.Type: ApplicationFiled: June 15, 2023Publication date: January 4, 2024Applicant: THE UNIVERSITY OF TOKYOInventors: Hiromitsu NAKAUCHI, Hideyuki SATO, Hideki MASAKI, Motoo WATANABE
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Patent number: 11856927Abstract: The present invention has found that chimeric animals suffer from noticeable inflammation after birth, though neither immune response nor inflammation in the fetal period of these animals has been reported hitherto. This is an unexpected finding since chimeric animals in the fetal period were exclusively analyzed in prior studies and thus it is deemed that immunotolerance has been theoretically established therein. The present invention provides a composition for suppressing immune response or inflammation in the fetal period of a born chimeric animal.Type: GrantFiled: January 25, 2018Date of Patent: January 2, 2024Assignee: The University of TokyoInventors: Hiromitsu Nakauchi, Tomoyuki Yamaguchi, Sanae Hamanaka, Hideyuki Sato, Hideki Masaki, Naoaki Mizuno, Motoo Watanabe
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Patent number: 11844336Abstract: The present invention provides a method for producing a chimeric animal using a primed pluripotent stem cell, a tissue stem cell, a progenitor cell, a somatic cell, or a germ cell. The method for producing a chimeric animal according to the present invention comprises introducing a mammal-derived cell into the embryo of a mammal, the cell being primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.Type: GrantFiled: April 24, 2020Date of Patent: December 19, 2023Assignees: THE UNIVERSITY OF TOKYO, THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Hiromitsu Nakauchi, Hideki Masaki, Motoo Watanabe, Irving Weissman
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Publication number: 20230180725Abstract: Methods of generating functional human organs and tissue in animal bodies suitable for transplantation into human subjects are provided. In particular, the contribution of human donor cells to tissues and organs can be increased in interspecies host embryos by knocking out a growth factor receptor gene such as the insulin-like growth factor 1 receptor or insulin receptor gene. Almost entirely donor-derived functional organs and tissue can be generated by using this method. The methods described herein are useful for generating human organs and tissue in animals and may be helpful for overcoming the current problems with organ shortage for transplantation therapy. Additionally, such organs and tissue can be used in drug discovery, drug screening, and toxicology testing.Type: ApplicationFiled: April 8, 2021Publication date: June 15, 2023Inventors: Toshiya Nishimura, Hiromitsu Nakauchi, Motoo Watanabe
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Publication number: 20230106769Abstract: The present invention discloses a serum-free medium and a culture method suitable for culturing human cells. The present invention provides a method of culturing human cells, the method comprising bringing the human cells into contact with polyalkylene glycol modified with a copolymer containing a polyvinylcaprolactam block and a polyvinylacetate block.Type: ApplicationFiled: January 22, 2021Publication date: April 6, 2023Inventors: Satoshi YAMAZAKI, Motoo WATANABE
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Publication number: 20230071538Abstract: Cytotoxic T cells derived from human T cell-derived iPS cells may avoid an NK cell missing-self response and may be used for allogeneic administration while maintaining a strong antitumor effect of antigen-specific CTLs. A method may produce a cytotoxic T cell derived from a human T cell-derived iPS cell expressing HLA class I of HLA-restricted class I of an antigen epitope of a CTL and HLA-E. Such a method may include: knocking out all HLA class I of the human T cell-derived iPS cell; introducing a gene of the HLA-restricted HLA class I of the antigen epitope of the CTL and a gene of the HLA-E into the T-iPS cell in which all HLA class I have been knocked out; and redifferentiating the genetically introduced T-iPS cell into a CD8 single-positive T cell.Type: ApplicationFiled: February 5, 2021Publication date: March 9, 2023Applicants: JUNTENDO EDUCATIONAL FOUNDATION, THE UNIVERSITY OF TOKYOInventors: Miki ANDO, Jun ANDO, Midori ISHII, Norio KOMATSU, Hiromitsu NAKAUCHI, Motoo WATANABE
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Publication number: 20220298479Abstract: The present invention discloses a composition of an albumin-free, serum-free medium suited for culturing human hematopoietic stem cells and an albumin-free culturing method. According to the present invention, a method of culturing human hematopoietic stem cells is provided which comprises bringing human hematopoietic stem cells into contact with PVA and a PI3K activator.Type: ApplicationFiled: September 11, 2020Publication date: September 22, 2022Applicant: The University of TokyoInventors: Satoshi YAMAZAKI, Motoo WATANABE, Masatoshi SAKURAI, Hiromitsu NAKAUCHI
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Patent number: 11432537Abstract: The present invention discloses a novel means capable of producing a blood chimeric animal in which a state of retaining blood cells originating in a heterologous animal at a high percentage is sustained for a long period of time. The method for producing a non-human animal that retains blood cells originating in a heterologous animal, according to the present invention, comprises transplanting hematopoietic cells of a heterologous animal into a non-human animal, in which hematopoietic cells the function of a gene that acts on the hematopoietic system is modified, The gene that acts on the hematopoietic system is, for example, Lnk gene, When a medium to large mammal is used as a recipient, the survival rate of hematopoietic cells originating in a heterologous animal is dramatically increased such that blood chimerism of 10% or more can be maintained even in a 16 month old animal.Type: GrantFiled: June 20, 2016Date of Patent: September 6, 2022Assignees: THE UNIVERSITY OF TOKYO, NATIONAL FEDERATION OF AGRICULTURAL COOPERTIVE ASSOCIATIONSInventors: Kazuo Fukawa, Tetsuya Ito, Mai Kamikawa, Yutaka Sendai, Hiromitsu Nakauchi, Satoshi Yamazaki, Motoo Watanabe
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Publication number: 20220160789Abstract: Provided is an immune cell therapy which uses an iPS technology allowing long-term survival in a living body and which exhibits an excellent antitumor effect by recognition of two antigens. An iPS cell derived from an antigen-specific cytotoxic T cell having a chimeric antigen receptor introduced therein.Type: ApplicationFiled: April 1, 2020Publication date: May 26, 2022Applicants: JUNTENDO EDUCATIONAL FOUNDATION, THE UNIVERSITY OF TOKYOInventors: Miki ANDO, Norio KOMATSU, Jun ANDO, Sakiko HARADA, Hiromitsu NAKAUCHI, Tomoyuki YAMAGUCHI, Motoo WATANABE
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Patent number: 10900092Abstract: The invention provides a primer set for detecting Chlamydia trachomatis containing (a) a first primer pair composed of a forward primer consisting of a base sequence represented by SEQ ID NO: 5 and (b) a reverse primer consisting of a base sequence represented by SEQ ID NO: 6, as well as a method for detecting Chlamydia trachomatis using the primer set, and a reagent kit therefor.Type: GrantFiled: March 27, 2017Date of Patent: January 26, 2021Assignee: FUJIFILM WAKO PURE CHEMICAL CORPORATIONInventors: Tatsuya Nakamura, Motoo Watanabe
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Publication number: 20200315148Abstract: The present invention provides a method for producing a chimeric animal using a primed pluripotent stem cell, a tissue stem cell, a progenitor cell, a somatic cell, or a germ cell. The method for producing a chimeric animal according to the present invention comprises introducing a mammal-derived cell into the embryo of a mammal, the cell being primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.Type: ApplicationFiled: April 24, 2020Publication date: October 8, 2020Applicants: THE UNIVERSITY OF TOKYO, THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Hiromitsu NAKAUCHI, Hideki MASAKI, Motoo WATANABE, Irving WEISSMAN
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Publication number: 20200315146Abstract: The present invention provides a method for preparing a somatic chimera by using a pluripotent cell genetically engineered not to differentiate into a predetermined type of cell. The present invention particularly provides a method for preventing a pluripotent cell from contributing to the brain and gonad in a somatic chimera animal.Type: ApplicationFiled: October 9, 2018Publication date: October 8, 2020Applicant: The University of TokyoInventors: Hiromitsu NAKAUCHI, Hideyuki SATO, Hideki MASAKI, Motoo WATANABE
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Patent number: 10645912Abstract: [Problem to be Solved] The present invention provides a method for producing a chimeric animal using a primed pluripotent stem cell, a tissue stem cell, a progenitor cell, a somatic cell, or a germ cell. [Solution] The method for producing a chimeric animal according to the present invention comprises introducing a mammal-derived cell into the embryo of a mammal, the cell being primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.Type: GrantFiled: January 17, 2018Date of Patent: May 12, 2020Assignees: THE UNIVERSITY OF TOKYO, THE BOARD OF REGENTS OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Hiromitsu Nakauchi, Hideki Masaki, Motoo Watanabe, Irving Weissman
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Publication number: 20200137991Abstract: The present invention has found that chimeric animals suffer from noticeable inflammation after birth, though neither immune response nor inflammation in the fetal period of these animals has been reported hitherto. This is an unexpected finding since chimeric animals in the fetal period were exclusively analyzed in prior studies and thus it is deemed that immunotolerance has been theoretically established therein. The present invention provides a composition for suppressing immune response or inflammation in the fetal period of a born chimeric animal.Type: ApplicationFiled: January 25, 2018Publication date: May 7, 2020Inventors: Hiromitsu NAKAUCHI, Tomoyuki YAMAGUCHI, Sanae HAMANAKA, Hideyuki SATO, Hideki MASAKI, Naoaki MIZUNO, Motoo WATANABE
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Publication number: 20190112638Abstract: The invention provides a primer set for detecting Chlamydia trachomatis containing (a) a first primer pair composed of a forward primer consisting of a base sequence represented by SEQ ID NO: 5 and (b) a reverse primer consisting of a base sequence represented by SEQ ID NO: 6, as well as a method for detecting Chlamydia trachomatis using the primer set, and a reagent kit therefor.Type: ApplicationFiled: March 27, 2017Publication date: April 18, 2019Applicant: FUJIFILM WAKO PURE CHEMICAL CORPORATIONInventors: Tatsuya NAKAMURA, Motoo WATANABE
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Publication number: 20180360004Abstract: [Problem to be Solved] The present invention provides a method for producing a chimeric animal using a primed pluripotent stem cell, a tissue stem cell, a progenitor cell, a somatic cell, or a germ cell. [Solution] The method for producing a chimeric animal according to the present invention comprises introducing a mammal-derived cell into the embryo of a mammal, the cell being primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.Type: ApplicationFiled: January 17, 2018Publication date: December 20, 2018Applicants: THE UNIVERSITY OF TOKYO, THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Hiromitsu Nakauchi, Hideki Masaki, Motoo Watanabe, Irving Weissman
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Publication number: 20180177164Abstract: The present invention discloses a novel means capable of producing a blood chimeric animal in which a state of retaining blood cells originating in a heterologous animal, such as a human, at a high percentage is sustained for a long period of time, even in cases where a medium to large domestic animal is used as a recipient. The method for producing a non-human animal that retains blood cells originating in a heterologous animal, according to the present invention, comprises transplanting hematopoietic cells of a heterologous animal into a non-human animal, in which hematopoietic cells the function of a gene that acts on the hematopoietic system is modified. The gene that acts on the hematopoietic system is, for example, Lnk gene.Type: ApplicationFiled: June 20, 2016Publication date: June 28, 2018Applicants: National Federation of Agricultural Cooperative Associations, The University of TokyoInventors: Kazuo FUKAWA, Tetsuya ITO, Mai KAMIKAWA, Yutaka SENDAI, Hiromitsu NAKAUCHI, Satoshi YAMAZAKI, Motoo WATANABE
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Publication number: 20160073616Abstract: [Problem to be Solved] The present invention provides a method for producing a chimeric animal using a primed pluripotent stem cell, a tissue stem cell, a progenitor cell, a somatic cell, or a germ cell. [Solution] The method for producing a chimeric animal according to the present invention comprises introducing a mammal-derived cell into the embryo of a mammal, the cell being primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.Type: ApplicationFiled: January 29, 2014Publication date: March 17, 2016Applicants: THE UNIVERSITY OF TOKYO, THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Hiromitsu NAKAUCHI, Hideki MASAKI, Motoo WATANABE, Irving WEISSMAN
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Patent number: RE43227Abstract: A method and system for monitoring and controlling machines, such as printers, copiers, facsimile machine and other image forming machines. A server is used for transmitting and communicating information among machines and computers. The server acquires, after machine installation, reference values and during operation, current condition information. The server evaluates this information with the reference values such that a response to the machine may be executed remotely. The server also may update the reference values which change during operation.Type: GrantFiled: April 2, 2007Date of Patent: March 6, 2012Assignee: Ricoh Company, LimitedInventors: Motoo Watanabe, Gregory Wright
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Patent number: RE43257Abstract: A method and system for monitoring and controlling machines, such as printers, copiers, facsimile machine and other image forming machines. A server is used for transmitting and communicating information among machines and computers. The server acquires, after machine installation, reference values and during operation, current condition information. The server evaluates this information with the reference values such that a response to the machine may be executed remotely. The server also may update the reference values which change during operation.Type: GrantFiled: July 1, 2005Date of Patent: March 20, 2012Assignee: Ricoh Company, LimitedInventors: Motoo Watanabe, Gregory Wright