Patents by Inventor Mototsugu Eiraku
Mototsugu Eiraku has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11542471Abstract: The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.Type: GrantFiled: August 14, 2018Date of Patent: January 3, 2023Assignees: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKENInventors: Tokushige Nakano, Satoshi Ando, Yoshiki Sasai, Mototsugu Eiraku
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Patent number: 11234434Abstract: The invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the steps of (1) bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and (3) cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution.Type: GrantFiled: November 22, 2012Date of Patent: February 1, 2022Assignees: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKENInventors: Satoshi Ando, Tokushige Nakano, Yoshiki Sasai, Mototsugu Eiraku
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Publication number: 20210228645Abstract: The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serumcontaining medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.Type: ApplicationFiled: April 12, 2021Publication date: July 29, 2021Applicants: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKENInventors: Tokushige NAKANO, Satoshi ANDO, Yoshiki SASAI, Mototsugu EIRAKU
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Publication number: 20210154370Abstract: The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.Type: ApplicationFiled: September 14, 2018Publication date: May 27, 2021Applicants: RIKEN, SUMITOMO DAINIPPON PHARMA CO., LTD., SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Daiki NUKAYA, Mototsugu EIRAKU, Yuiko KINOSE, Akishi ONISHI, Masayo TAKAHASHI, Yoshiki SASAI (deceased)
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Publication number: 20210115399Abstract: Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming homogenous aggregates of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregates of stem cells in the presence of a basement membrane reference standard.Type: ApplicationFiled: October 5, 2020Publication date: April 22, 2021Applicants: RIKEN, Sumitomo Chemical Company, LimitedInventors: Yoshiki SASAI, Mototsugu EIRAKU
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Patent number: 10973854Abstract: The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serum-containing medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.Type: GrantFiled: November 22, 2012Date of Patent: April 13, 2021Assignees: Sumitomo Chemical Company, Limited, RIKENInventors: Tokushige Nakano, Satoshi Ando, Yoshiki Sasai, Mototsugu Eiraku
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Patent number: 10934523Abstract: The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming a homogenous aggregate of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as a suspended aggregate in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.Type: GrantFiled: February 15, 2019Date of Patent: March 2, 2021Assignee: RIKENInventors: Yoshiki Sasai, Takafumi Wataya, Mototsugu Eiraku
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Patent number: 10810407Abstract: A specific region such as a continuous epithelial structure is automatically detected in a cell aggregate. A region detecting method includes an input step (S01) of inputting an image obtained by imaging a cell aggregate, an outline detecting step (S02) of detecting an outline of the cell aggregate together with an order along the outline from the input image, a region detecting step (S03) of detecting a specific region included in the cell aggregate based on the detected outline and order, and an output step (S04) of outputting information indicating the detected region.Type: GrantFiled: November 25, 2016Date of Patent: October 20, 2020Assignees: RIKEN, Sumitomo Dainippon Pharma Co., Ltd., Sumitomo Chemical Company, LimitedInventors: Satoru Okuda, Mototsugu Eiraku, Atsushi Kuwahara
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Patent number: 10793830Abstract: Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.Type: GrantFiled: November 5, 2010Date of Patent: October 6, 2020Assignee: RIKENInventors: Yoshiki Sasai, Mototsugu Eiraku
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Publication number: 20200277571Abstract: The present invention aims to provide a retinal tissue rich in cone photoreceptor precursors and/or cone photoreceptors, a retinal tissue rich in rod photoreceptor precursors and/or rod photoreceptors, and a production method thereof and the like.Type: ApplicationFiled: September 14, 2018Publication date: September 3, 2020Applicants: RIKEN, SUMITOMO DAINIPPON PHARMA CO., LTD., SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Daiki NUKAYA, Mototsugu EIRAKU, Yuiko KINOSE, Akishi ONISHI, Masayo TAKAHASHI, Yoshiki SASAI (deceased)
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Publication number: 20200208103Abstract: The present invention provides a method for maintaining a continuous epithelial structure of a retinal tissue including culturing the retinal tissue in a medium comprising a methyl group donor or a substrate of the methyl group donor at a concentration at which cell differentiation of a neural retinal progenitor cell is suppressed, and a neurite extension inhibitor at a concentration at which neurite extension is suppressed.Type: ApplicationFiled: July 20, 2018Publication date: July 2, 2020Applicants: RIKEN, SUMITOMO DAINIPPON PHARMA CO., LTD., SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Hideya SAKAGUCHI, Yoshiki SASAI (deceased), Mototsugu EIRAKU, Daiki NUKAYA, Atsushi KUWAHARA
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Publication number: 20190218513Abstract: The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming a homogenous aggregate of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as a suspended aggregate in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells fromType: ApplicationFiled: February 15, 2019Publication date: July 18, 2019Applicant: RIKENInventors: Yoshiki SASAI (deceased), Takafumi WATAYA, Mototsugu EIRAKU
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Patent number: 10227563Abstract: The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming homogenous aggregates of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as suspended aggregates in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.Type: GrantFiled: June 5, 2009Date of Patent: March 12, 2019Assignee: RIKENInventors: Yoshiki Sasai, Takafumi Wataya, Mototsugu Eiraku
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Publication number: 20180371409Abstract: The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.Type: ApplicationFiled: August 14, 2018Publication date: December 27, 2018Applicants: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKENInventors: Tokushige NAKANO, Satoshi ANDO, Yoshiki SASAI, Mototsugu EIRAKU
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Publication number: 20180349672Abstract: A specific region such as a continuous epithelial structure is automatically detected in a cell aggregate. A region detecting method includes an input step (S01) of inputting an image obtained by imaging a cell aggregate, an outline detecting step (S02) of detecting an outline of the cell aggregate together with an order along the outline from the input image, a region detecting step (S03) of detecting a specific region included in the cell aggregate based on the detected outline and order, and an output step (S04) of outputting information indicating the detected region.Type: ApplicationFiled: November 25, 2016Publication date: December 6, 2018Applicants: RIKEN, Sumitomo Dainippon Pharma Co., Ltd., SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Satoru Okuda, Mototsugu Eiraku, Atsushi Kuwahara
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Patent number: 10077425Abstract: The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.Type: GrantFiled: June 7, 2013Date of Patent: September 18, 2018Assignees: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKENInventors: Tokushige Nakano, Satoshi Ando, Yoshiki Sasai, Mototsugu Eiraku
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Publication number: 20150132787Abstract: The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.Type: ApplicationFiled: June 7, 2013Publication date: May 14, 2015Applicant: RIKENInventors: Yoshiki Sasai, Satoshi Ando, Mototsugu Eiraku, Tokushige Nakano
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Publication number: 20140342346Abstract: The invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the steps of (1) bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and (3) cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution.Type: ApplicationFiled: November 22, 2012Publication date: November 20, 2014Inventors: Satoshi Ando, Tokushige Nakano, Yoshiki Sasai, Mototsugu Eiraku
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Publication number: 20140341864Abstract: The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serum-containing medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.Type: ApplicationFiled: November 22, 2012Publication date: November 20, 2014Inventors: Tokushige Nakano, Satoshi Ando, Yoshiki Sasai, Mototsugu Eiraku
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Publication number: 20130040330Abstract: Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.Type: ApplicationFiled: November 5, 2010Publication date: February 14, 2013Applicant: RIKENInventors: Yoshiki Sasai, Mototsugu Eiraku