Patents by Inventor Nader Pourmand
Nader Pourmand has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11940410Abstract: Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4 ng/ml is also disclosed, as is detection of VEGF.Type: GrantFiled: January 18, 2022Date of Patent: March 26, 2024Assignee: The Regents of the University of CaliforniaInventors: Miloslav Karhanek, Chris David Webb, Senkei Umehara, Nader Pourmand
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Patent number: 11709148Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.Type: GrantFiled: December 21, 2021Date of Patent: July 25, 2023Assignee: The Regents of the University of CaliforniaInventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger
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Publication number: 20220260520Abstract: Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4 ng/ml is also disclosed, as is detection of VEGF.Type: ApplicationFiled: January 18, 2022Publication date: August 18, 2022Inventors: Miloslav Karhanek, Chris David Webb, Senkei Umehara, Nader Pourmand
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Publication number: 20220229014Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.Type: ApplicationFiled: December 21, 2021Publication date: July 21, 2022Inventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger
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Patent number: 11255814Abstract: Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4 ng/ml is also disclosed, as is detection of VEGF.Type: GrantFiled: September 30, 2019Date of Patent: February 22, 2022Assignee: The Regents of the University of CaliforniaInventors: Miloslav Karhanek, Chris David Webb, Senkei Umehara, Nader Pourmand
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Patent number: 11243188Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.Type: GrantFiled: May 24, 2019Date of Patent: February 8, 2022Assignee: The Regents of the University of CaliforniaInventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger
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Publication number: 20210230678Abstract: Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal.Type: ApplicationFiled: November 2, 2020Publication date: July 29, 2021Inventors: Nader Pourmand, Miloslav Karhanek, Ronald W. Davis
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Publication number: 20210041426Abstract: Provided are methods for simultaneously detecting analytes. The methods include exposing a tip of a nanosensor to a biological sample, measuring ionic current flow through the nanosensor tip to detect a first analyte and a second analyte in the biological sample, and distinguishing the first analyte from the second analyte based on a first diffusion limited current peak resulting from binding of a first specific binding member to the first analyte being distinguishable from a second diffusion limited current peak resulting from binding of a second specific binding member to the second analyte. Sensing apparatuses that find use, e.g., in practicing the methods of the present disclosure, are also provided.Type: ApplicationFiled: February 11, 2019Publication date: February 11, 2021Inventor: Nader Pourmand
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Patent number: 10822641Abstract: Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal.Type: GrantFiled: April 30, 2018Date of Patent: November 3, 2020Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Nader Pourmand, Miloslav Karhanek, Ronald W. Davis
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Patent number: 10809253Abstract: Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.Type: GrantFiled: December 4, 2017Date of Patent: October 20, 2020Assignees: MagArray, Inc., The Board of Trustees of the Leland Stanford Junior UniversityInventors: Shan Xiang Wang, Sebastian J. Osterfeld, Heng Yu, Nader Pourmand, Robert L. White
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Patent number: 10696962Abstract: Described herein are devices and methods for extracting cellular material from living cells and then depositing them into to a receptacle in a nanoliter scale. Using a nanopipette integrated into a scanning ion conductance microscope (SICM), extraction of mitochondrial DNA from human BJ fibroblasts and Green Fluorescent Protein (GFP) transcripts from HeLa/GFP cells was achieved with minimal disruption to the cellular milieu and without chemical treatment prior to obtaining the isolated sample. Success of the extraction was confirmed by fluorescence microscopy and PCR analysis of the extracted material. The method and apparatus may be applied to many different cell types and intracellular targets, allowing not only single cell analysis, but single subcellular compartment analysis of materials extracted in their native state.Type: GrantFiled: March 13, 2014Date of Patent: June 30, 2020Assignee: The Regents of the University of CaliforniaInventors: Paolo Actis, Michelle M. Maalouf, Nader Pourmand
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Publication number: 20200200704Abstract: Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4 ng/ml is also disclosed, as is detection of VEGF.Type: ApplicationFiled: March 11, 2019Publication date: June 25, 2020Inventors: Miloslav Karhanek, Chris David Webb, Senkei Umehara, Nader Pourmand
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Publication number: 20200182827Abstract: Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4 ng/ml is also disclosed, as is detection of VEGF.Type: ApplicationFiled: September 30, 2019Publication date: June 11, 2020Inventors: Miloslav Karhanek, Chris David Webb, Senkei Umehara, Nader Pourmand
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Patent number: 10513434Abstract: Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.Type: GrantFiled: March 20, 2017Date of Patent: December 24, 2019Assignee: The Regents of the University of CaliforniaInventors: R. Adam Seger, Paolo Actis, Boaz Vilozny, Nader Pourmand
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Publication number: 20190369047Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.Type: ApplicationFiled: May 24, 2019Publication date: December 5, 2019Inventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger
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Patent number: 10345260Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.Type: GrantFiled: March 6, 2015Date of Patent: July 9, 2019Assignee: The Regents of the University of CaliforniaInventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger
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Publication number: 20180346971Abstract: Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal.Type: ApplicationFiled: April 30, 2018Publication date: December 6, 2018Inventors: Nader Pourmand, Miloslav Karhanek, Ronald W. Davis
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Publication number: 20180259480Abstract: Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4ng/ml is also disclosed, as is detection of VEGF.Type: ApplicationFiled: September 6, 2017Publication date: September 13, 2018Inventors: Miloslav Karhanek, Chris David Webb, Senkei Umehara, Nader Pourmand
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Publication number: 20180128822Abstract: Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.Type: ApplicationFiled: December 4, 2017Publication date: May 10, 2018Inventors: Shan Xiang Wang, Sebastian J. Osterfeld, Heng Yu, Nader Pourmand, Robert L. White
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Publication number: 20180045675Abstract: Disclosed is a method and device for sensing pH in a single living cell. The device is constructed for directing a nano-sized probe to pierce a single cell and extract accurate pH measurements in real time therefrom. A nanopipette, containing an electrode, is prepared through physisorption of chitosan, a biocompatible pH-responsive polymer, onto highly hydroxylated quartz nanopipettes with extremely small pore size (?97 nm). Changes of pH alter the surface charge of chitosan, which can be measured as a change in ionic current at the nanopore. The dynamic pH range of the nano-pH probe was from 2.6 to 10.7 with a sensitivity of 0.09 pH units. The present device can be used for single-cell intracellular pH measurements using, for example, non-cancerous and cancerous human cells, including human fibroblasts and model cells such as HeLa (epithelial cervix).Type: ApplicationFiled: February 24, 2016Publication date: February 15, 2018Inventors: Rifat Emrah OZEL, Nader POURMAND