Patents by Inventor Nadezda Fomina
Nadezda Fomina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11867660Abstract: Device and methods for controlling pH or ionic gradient comprising a multisite array of feedback electrode sets comprising electrodes and pH sensing elements. The electrodes can include a reference electrode, counter electrode, and a working electrode. The device and methods iteratively select an amount of current and/or voltage to be applied to each working electrode, apply the selected amount of current and/or voltage to each working electrode to change pH of a solution close to the working electrode, and measure the signal output of the sensing element. The multisite array can include feedback and non-feedback electrode sets.Type: GrantFiled: July 17, 2020Date of Patent: January 9, 2024Assignee: ROBERT BOSCH GMBHInventors: Christopher Johnson, Sam Kavusi, Nadezda Fomina, Habib Ahmad, Autumn Maruniak, Christoph Lang, Ashwin Raghunathan, Young Shik Shin, Armin Darvish, Efthymios Papageorgiou
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Patent number: 11814675Abstract: The present disclosure relates to systems, devices, and methods for nucleic acid sequencing including polynucleotide strands having a nucleotide(s) modified with a redox label(s) attached thereto or capable of receiving the modified nucleotide(s) with a redox label(s) attached thereto. The systems, devices, and methods include a dielectric member with an attached translocating protein positioned between oxidizing and reducing electrodes.Type: GrantFiled: August 29, 2019Date of Patent: November 14, 2023Assignee: Robert Bosch GmbHInventors: Nadezda Fomina, Christopher Johnson, Young Shik Shin, Christoph Lang
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Publication number: 20230334203Abstract: A computational method for characterizing a biopolymer property of a biopolymer. The computational method includes receiving a dimensional representation of a molecule concentration over time within a fluid flow of a fluid medium flowing through a fluid channel including the biopolymer; predicting a fluid flow velocity and/or a fluid flow pressure of the fluid medium in response to the dimensional representation of the molecule concentration over time within the fluid medium using a machine learning model; and characterizing the biopolymer property of the biopolymer in response to the fluid flow velocity and/or the fluid flow pressure.Type: ApplicationFiled: April 19, 2022Publication date: October 19, 2023Inventors: Giovanna BUCCI, Young Shik SHIN, Carlos CUNHA, Karim GADELRAB, Christopher JOHNSON, Nadezda FOMINA
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Publication number: 20230151419Abstract: A method of sequencing a prepared DNA strand includes providing the prepared DNA strand with nucleotides of the types dATP, dCTP, dGTP and/or dTTP, wherein at least one of the types of the nucleotides comprises a predetermined magnetic label. The method further includes placing the prepared DNA strand within a measuring range of a sensor unit comprising an operatively connected magneto-optical transducer unit and an optical sensor and optically exciting the magneto-optical transducer unit. The method continues with acquiring at least one value indicative of a fluorescence signal of the magneto-optical transducer unit, and assigning the acquired value to the at least one type of the nucleotides comprising the predetermined magnetic label.Type: ApplicationFiled: October 5, 2022Publication date: May 18, 2023Inventors: Andreas Brenneis, Felix Michael Stuerner, Jochen Hoffmann, Nadezda Fomina, Robert Roelver, Tino Fuchs, Christian Grumaz
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Patent number: 11592420Abstract: Closed-loop systems and methods for controlling pH. The system includes a working electrode, a counter electrode, a reference electrode, a first ion-sensitive field-effect transistor (ISFET), a second ISFET, and an electronic controller. The working electrode, the counter electrode, the reference electrode, and a first sensing terminal of the first ISFET are immersible in an active solution. A second sensing terminal of the second ISFET is immersible in a reference solution. The electronic controller is configured to apply a first amount of current or voltage to the working electrode and determine a differential voltage between the first ISFET and the second ISFET. The electronic controller is also configured to set a second amount of current or voltage to reduce a difference between the differential voltage and a target voltage. The electronic controller is further configured to apply the second amount of current or voltage to the working electrode.Type: GrantFiled: July 17, 2020Date of Patent: February 28, 2023Assignee: ROBERT BOSCH GMBHInventors: Young Shik Shin, Nadezda Fomina, Christopher Johnson, Armin Darvish, Efthymios Papageorgiou, Christoph Lang
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Patent number: 11561198Abstract: Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.Type: GrantFiled: December 20, 2018Date of Patent: January 24, 2023Assignee: ROBERT BOSCH GMBHInventors: Christopher Johnson, Sam Kavusi, Nadezda Fomina, Habib Ahmad, Autumn Maruniak, Christoph Lang, Ashwin Raghunathan
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Publication number: 20220268729Abstract: Provided is a method for monitoring a change of ion strength in a sample solution by a closed-loop device that provides continuous cycling of electrochemical pH modulation between pre-defined pH values. In particular, the change of ion strength may be induced by a chemical reaction and may ultimately alter the electrical control parameters of the closed-loop device. By measuring such electrical control parameters, the degree and progress of the underlying chemical reaction may be monitored.Type: ApplicationFiled: February 24, 2021Publication date: August 25, 2022Inventors: Young Shik Shin, Nadezda Fomina, Christopher Johnson, Armin Darvish, Christoph Lang
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Publication number: 20220018806Abstract: Closed-loop systems and methods for controlling pH. The system includes a working electrode, a counter electrode, a reference electrode, a first ion-sensitive field-effect transistor (ISFET), a second ISFET, and an electronic controller. The working electrode, the counter electrode, the reference electrode, and a first sensing terminal of the first ISFET are immersible in an active solution. A second sensing terminal of the second ISFET is immersible in a reference solution. The electronic controller is configured to apply a first amount of current or voltage to the working electrode and determine a differential voltage between the first ISFET and the second ISFET. The electronic controller is also configured to set a second amount of current or voltage to reduce a difference between the differential voltage and a target voltage. The electronic controller is further configured to apply the second amount of current or voltage to the working electrode.Type: ApplicationFiled: July 17, 2020Publication date: January 20, 2022Inventors: Young Shik Shin, Nadezda Fomina, Christopher Johnson, Armin Darvish, Efthymios Papageorgiou, Christoph Lang
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Publication number: 20210403642Abstract: A bifunctional polymer is functionalized at one end with an azlactone end group to conjugate biomolecules of interest, and is functionalized at another end with an azide anchor group to attach the polymer to a substrate. Methods of making the bifunctional polymer are also provided. A coated substrate includes the bifunctionalized polymers on the surface of a substrate. Methods of making the coated substrate are also provided. A microarray includes a plurality of discrete regions, each region including the coated substrate.Type: ApplicationFiled: June 8, 2021Publication date: December 30, 2021Inventors: Nadezda Fomina, Autumn Maruniak, Christopher Johnson, Habib Ahmad, Sam Kavusi
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Publication number: 20210364506Abstract: A method for detecting an amount of an analyte in a solution includes adding an electrochemically active agent to the solution; applying an electrical current to a working electrode in contact with the solution to initiate the electrochemical redox reaction to change a pH of the solution from a first pH value to a second pH value; incubating the solution at the second pH value to allow the analyte to dissociate from the other molecules in the solution and to bind to a capture molecule connected to the working electrode via a linker; reacting a detecting probe with the analyte to allow the detecting probe to bind to the analyte, the detecting probe having a signaling tag attached thereto and configured to produce a signal at the second pH value; and collecting the signal to calculate the amount of the analyte in the solution.Type: ApplicationFiled: May 22, 2020Publication date: November 25, 2021Inventors: Nadezda FOMINA, Christopher JOHNSON, Young Shik SHIN, Christoph LANG
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Patent number: 11131646Abstract: A device to electrochemically sequence DNA that includes a redox species includes at least one edge electrode, at least one stack of insulator material, and a pair of DNA translocation electrodes including a DNA translocation working electrode and a DNA translocation counter electrode, where the thickness of the at least one edge electrode is about 0.5 nanometers, and the thickness of the at least one stack of insulator material is about 10 nanometers. Methods of electrochemically sequencing a strand of DNA are also provided.Type: GrantFiled: June 15, 2018Date of Patent: September 28, 2021Assignee: ROBERT BOSCH GMBHInventors: Christopher Johnson, Habib Ahmad, Nadezda Fomina, Gary Yama, Franz Laermer, Jochen Hoffmann, Patrick Staley, Christoph Lang, Young Shik Shin
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Patent number: 11035822Abstract: A sensor and a corresponding method measure the pH of a solution without using an external reference electrode. The pH sensor has at least one functionalized electrode with an attached first redox active molecule and an attached second redox active molecule that are differently sensitive to pH and oxidize/reduce at different voltages, and a difference in peaks of a response of the two molecules to applied electrical parameter is measured and correlated to a particular pH.Type: GrantFiled: September 6, 2018Date of Patent: June 15, 2021Assignee: ROBERT BOSCH GMBHInventors: Patrick Staley, Nadezda Fomina, Murat Deliomeroglu, Christopher Johnson, Young Shik Shin
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Patent number: 10961563Abstract: A method of fabricating a nanoscale topography system for inducing unfolding of a DNA molecule for sequencing includes providing a substrate and creating trench walls on the substrate which define a trench therebetween. The method further includes depositing a layer of a block copolymer (BCP) in the trench and forming cylindrical domains by self-assembly of the BCP between the trench walls, removing a first portion of the cylindrical domains to create a vacant region in the trench, and depositing a subsequent layer of the BCP in the vacant region and forming spherical domains by self-assembly of the BCP between the trench walls adjacent a second portion of the cylindrical domains. The spherical domains form staggered post structures for unfolding the DNA molecule and the cylindrical domains form parallel channel structures for entry of the DNA molecule for sequencing.Type: GrantFiled: December 19, 2019Date of Patent: March 30, 2021Assignee: Robert Bosch GmbHInventors: Karim Gadelrab, Giovanna Bucci, Nathan Craig, Christopher Johnson, Nadezda Fomina, Young Shik Shin
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Patent number: 10948413Abstract: An apparatus to detect more than one analyte in a solution comprising at least one electrode in contact with the solution, at least two dyes including a first dye and a second dye, and an electrochemically active agent, where the solution has a pH, the electrode is configured to modulate the pH of the solution by oxidizing or reducing the electrochemically active agent, the first dye and the second dye fluoresce at different pH levels, fluorescence of the first dye is used to indicate the presence of a first analyte, and fluorescence of the second dye is used to indicate the presence of a second analyte. Methods of detecting multiple analytes in a solution are also provided.Type: GrantFiled: December 21, 2017Date of Patent: March 16, 2021Assignee: ROBERT BOSCH GMBHInventors: Nadezda Fomina, Habib Ahmad, Christopher Johnson, Christoph Lang, Patrick Staley, Juergen Steigert, Franz Laermer
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Patent number: 10942146Abstract: Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.Type: GrantFiled: April 3, 2017Date of Patent: March 9, 2021Assignee: ROBERT BOSCH GMBHInventors: Christopher Johnson, Sam Kavusi, Nadezda Fomina, Habib Ahmad, Autumn Maruniak, Christoph Lang, Ashwin Raghunathan
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Publication number: 20210062255Abstract: The present disclosure relates to systems, devices, and methods for nucleic acid sequencing including polynucleotide strands having a nucleotide(s) modified with a redox label(s) attached thereto or capable of receiving the modified nucleotide(s) with a redox label(s) attached thereto. The systems, devices, and methods include a dielectric member with an attached translocating protein positioned between oxidizing and reducing electrodes.Type: ApplicationFiled: August 29, 2019Publication date: March 4, 2021Inventors: Nadezda FOMINA, Christopher JOHNSON, Young Shik SHIN, Christoph LANG
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Patent number: 10843192Abstract: At least one electrode is integrated on a lab on a chip cartridge in a sample preparation chamber of the cartridge, a DNA hybridization chamber of the cartridge, a protein assay chamber of the cartridge, and/or a detection chamber of the cartridge, for example, where the electrode is used to generate pH electrochemically in order to activate, deactivate, or intermediately attenuate an enzyme's activity on demand, in order to increase the fidelity of analyte detection, for cell lysis, for protein extraction, for DNA dehybridization, for primer hybridization control, for sample pre concentration, and/or for washing to remove non target species.Type: GrantFiled: November 4, 2016Date of Patent: November 24, 2020Assignee: ROBERT BOSCH GMBHInventors: Nadezda Fomina, Christopher Johnson, Habib Ahmad, Christoph Lang, Franz Laermer
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Publication number: 20200363371Abstract: Device and methods for controlling pH or ionic gradient comprising a multisite array of feedback electrode sets comprising electrodes and pH sensing elements. The electrodes can include a reference electrode, counter electrode, and a working electrode. The device and methods iteratively select an amount of current and/or voltage to be applied to each working electrode, apply the selected amount of current and/or voltage to each working electrode to change pH of a solution close to the working electrode, and measure the signal output of the sensing element. The multisite array can include feedback and non-feedback electrode sets.Type: ApplicationFiled: July 17, 2020Publication date: November 19, 2020Inventors: Christopher Johnson, Sam Kavusi, Nadezda Fomina, Habib Ahmad, Autumn Maruniak, Christoph Lang, Ashwin Raghunathan, Young Shik Shin, Armin Darvish, Efthymios Papageorgiou
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Patent number: 10823694Abstract: A method for detecting a presence and/or a concentration of a target substance in a reagent solution using enzyme-linked immunosorbent assay (ELISA) includes binding the target substance directly or indirectly to an electrode, and binding a detection agent directly or indirectly to the bound target substance. The method further includes modulating a pH of only a portion of the reagent solution in which the bound target substance and the bound detection agent are located using the electrode, the modulated pH of the portion of the reagent solution causing the bound detection agent to undergo a change, and detecting the change in the bound detection agent. The detected change corresponds to the presence of the target substance in the reagent solution and/or the concentration of the target substance in the reagent solution.Type: GrantFiled: July 11, 2018Date of Patent: November 3, 2020Assignee: Robert Bosch GmbHInventors: Young Shik Shin, Nadezda Fomina, Christopher Johnson, Christoph Lang, Patrick Staley
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Publication number: 20200080964Abstract: A sensor and a corresponding method measure the pH of a solution without using an external reference electrode. The pH sensor has at least one functionalized electrode with an attached first redox active molecule and an attached second redox active molecule that are differently sensitive to pH and oxidize/reduce at different voltages, and a difference in peaks of a response of the two molecules to applied electrical parameter is measured and correlated to a particular pH.Type: ApplicationFiled: September 6, 2018Publication date: March 12, 2020Inventors: Patrick Staley, Nadezda Fomina, Murat Deliomeroglu, Christopher Johnson, Young Shik Shin