Patents by Inventor Nagako Kawashima

Nagako Kawashima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methylotrophic yeast producing mammalian type sugar chain
    Patent number: 7972809
    Abstract: This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an ?-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the ?-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity.
    Type: Grant
    Filed: April 28, 2003
    Date of Patent: July 5, 2011
    Assignees: National Institute of Advanced Industrial Science & Technology, Kirin Beer Kabushiki Kaisha
    Inventors: Kazuo Kobayashi, Yoshinori Kitagawa, Toshihiro Komeda, Nagako Kawashima, Yoshifumi Jigami, Yasunori Chiba
  • Methylotroph producing mammalian type sugar chain
    Publication number: 20060148039
    Abstract: This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an ?-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the ?-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity.
    Type: Application
    Filed: April 28, 2003
    Publication date: July 6, 2006
    Inventors: Kazuo Kobayashi, Yoshinori Kitagawa, Toshihiro Komeda, Nagako Kawashima, Yoshifumi Jigami, Yasumori Chiba