Abstract: Methods for transforming monocot leaf explants are provided.

Abstract: Methods for transforming dicot vegetative plant organs and their composite tissues are provided.

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays PR1 gene. Some embodiments relate to a promoter or a 5? UTR from a Zea mays PR1 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3? UTR or a terminator from a Zea mays PR1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Zea mays chlorophyll a/b binding protein gene. Some embodiments relate to a 3? UTR from a Zea mays chlorophyll a/b binding protein gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Zea mays chlorophyll a/b binding protein gene. Some embodiments relate to a 3? UTR from a Zea mays chlorophyll a/b binding protein gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Oryza sativa Ubiquitin-3 gene. Some embodiments relate to a 3? UTR from an Oryza sativa Ubiquitin-3 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: A genetic screen for Arabidopsis mutants displaying a hyper-susceptible to Agrobacterium transformation (hat) phenotype was performed. The gene disrupted in the hat3 mutant encodes a putative myb-family transcription factor (MTF) that negatively regulates susceptibility to Agrobacterium-mediated transformation. Over-expression of an integrin-like protein results in plants that are hyper-susceptible to transformation. Manipulation of MTF, members of this protein family, and members of the integrin domain-like protein family for example At14a allows improved control of Agrobacterium transformation, including in crops.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.

Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

Abstract: A genetic screen for Arabidopsis mutants displaying a hyper-susceptible to Agrobacterium transformation (hat) phenotype was performed. The gene disrupted in the hat3 mutant encodes a putative myb-family transcription factor (MTF) that negatively regulates susceptibility to Agrobacterium-mediated transformation. Over-expression of an integrin-like protein results in plants that are hyper-susceptible to transformation. Manipulation of MTF, members of this protein family, and members of the integrin domain-like protein family for example At14a allows improved control of Agrobacterium transformation, including in crops.

Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.

Abstract: A genetic screen for Arabidopsis mutants displaying a hyper-susceptible to Agrobacterium transformation (hat) phenotype was performed. The gene disrupted in the hat3 mutant encodes a putative myb-family transcription factor (MTF) that negatively regulates susceptibility to Agrobacterium-mediated transformation. Over-expression of an integrin-like protein results in plants that are hyper-susceptible to transformation. Manipulation of MTF, members of this protein family, and members of the integrin domain-like protein family for example At14a allows improved control of Agrobacterium transformation, including in crops.

Abstract: The present invention relates to fine mapping and potential application of dna markers linked to a gall midge resistance gene gm7 for marker-aided selection in rice. Towards this, the present invention discloses a combination of novel sequence characterized amplified region (SCAR) primers for use in assay with the DNA of Rice plants in question. A cross between the gall midge resistant parent, RP2333 carrying the Gm7 gene and susceptible parent Shyamala, is developed and a F5 progeny is raised. A polymorphic band is identified from the F5 progeny, using AFLP that cosegregates with the susceptible phenotype. This band is eluted from the gel and cloned. The cloned AFLP fragment is sequenced and primers are developed for selectively amplifying DNA of susceptible phenotypes, thus differentiating them from the resistant phenotypes.