Abstract: There is provided a method of an animal model experiment to observe cells implanted to an experimental small animal in which the position or spatial spread only of the implanted cells can be detected and observed with time progress and with sufficient accuracy while a plurality of kinds of the implanted cells can be distinguished from one another. The inventive method of in vivo detecting implanted cells implanted into the body of a small animal comprises steps of preparing cells used as the implanted cells into which a gene expressing a tag of a part of a peptide or a protein on a cell membrane is introduced; implanting the implanted cells to an arbitrary site of the small animal; and detecting the tags in the small animal. The tags may be detected by dosing the small animal with fluorescently labeled antibodies to the tags and imaging the fluorescence from the antibodies.

Abstract: There is provided a method for detecting a reaction of a fluorescently labeled protein and a sample simply, in a short time and at a higher precision. By using an expression system such as an extracellular gene expression system and an extracellular transcription or translation system, a reaction of expressing a protein from a constructed vector is performed. At this time, a fluorescently labeled amino acid is introduced into the expression system in the form of a tRNA having the fluorescently labeled amino acid. With expression of a protein, the fluorescently labeled amino acid is incorporated into the protein, and a fluorescently labeled protein (hereafter, referred to as fluorescently labeled protein) is produced. The fluorescently labeled protein and a sample S are mixed to prepare a mixed solution, and FCS measurement is performed. Based on a measured value of the FCS, the presence or absence of a reaction is detected.

Abstract: A method for detecting binding of a nucleic acid and a nucleic acid binding protein by monomolecular fluorescent analysis.

Abstract: A method of detecting presence/absence of binding capacity of a test substance, with respect to a protein, comprising, (1) having a protein, which has been labeled with a fluorescence material, exist in a solution, and (2) while successively measuring fluorescence intensity from the fluorescent material, reacting the test substance with the fluorescence-labeled protein described in (1) above and determining presence/absence of binding capacity of the test substance, with respect to the protein, on the basis of the successive change in fluorescence intensity.

Abstract: Qualitative and quantitative methods for detecting the presence of double-stranded, non-5'-phosphorylated DNA in samples that may also contain 5'-phosphorylated DNA and/or single-stranded DNA are described. These methods involve treating the sample with an enzyme that specifically degrades 5'-phosphorylated DNA together with an enzyme that specifically degrades single-stranded DNA. More specifically, methods are described for the detection of the products of DNA amplification reactions, such as the polymerase chain reaction (PCR), wherein post-amplification enzyme digestion substantially reduces or eliminates background signals that are apparently caused by the presence of template DNA and primers in the sample after amplification is complete.