Patents by Inventor Naoto Tonouchi

Naoto Tonouchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Heparosan-producing bacterium and heparosan manufacturing method
    Patent number: 10611804
    Abstract: A method for producing heparosan is provided. Heparosan is produced by culturing an Escherichia bacterium having a heparosan-producing ability and modified so that expression of one or more genes, such as rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybil, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO, is/are increased in a medium, and collecting heparosan from the medium.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: April 7, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shunsuke Yamazaki, Tomoko Shimizu, Kenichi Mori, Naoto Tonouchi
  • Heparosan-Producing Bacterium and Heparosan Manufacturing Method
    Publication number: 20180237479
    Abstract: A method for producing heparosan is provided. Heparosan is produced by culturing an Escherichia bacterium having a heparosan-producing ability and modified so that expression of one or more genes, such as rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybil, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO, is/are increased in a medium, and collecting heparosan from the medium.
    Type: Application
    Filed: April 20, 2018
    Publication date: August 23, 2018
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shunsuke Yamazaki, Tomoko Shimizu, Kenichi Mori, Naoto Tonouchi
  • Heparosan-producing bacterium and heparosan manufacturing method
    Patent number: 9975928
    Abstract: A method for producing heparosan is provided. Heparosan is produced by culturing an Escherichia bacterium having a heparosan-producing ability and modified so that expression of one or more genes, such as rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybiI, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO, is/are increased in a medium, and collecting heparosan from the medium.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: May 22, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shunsuke Yamazaki, Tomoko Shimizu, Kenichi Mori, Naoto Tonouchi
  • Heparosan-Producing Bacterium and Heparosan Manufacturing Method
    Publication number: 20160201103
    Abstract: A method for producing heparosan is provided. Heparosan is produced by culturing an Escherichia bacterium having a heparosan-producing ability and modified so that expression of one or more genes, such as rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybiI, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO, is/are increased in a medium, and collecting heparosan from the medium.
    Type: Application
    Filed: March 28, 2016
    Publication date: July 14, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shunsuke Yamazaki, Tomoko Shimizu, Kenichi Mori, Naoto Tonouchi
  • Enzyme that catalyzes a peptide-forming reaction from a carboxy component and an amine component, microbe producing the same, and a method of producing a dipeptide using the enzyme or microbe
    Patent number: 8753841
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: December 13, 2012
    Date of Patent: June 17, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Enzyme that catalyzes a peptide-forming reaction from a carboxy component and an amine component, microbe producing the same, and a method of producing a dipeptide using the enzyme or microbe
    Patent number: 8389241
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: September 12, 2012
    Date of Patent: March 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • ENZYME THAT CATALYZES A PEPTIDE-FORMING REACTION FROM A CARBOXY COMPONENT AND AN AMINE COMPONENT, MICROBE PRODUCING THE SAME, AND A METHOD OF PRODUCING A DIPEPTIDE USING THE ENZYME OR MICROBE
    Publication number: 20130011873
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Application
    Filed: September 12, 2012
    Publication date: January 10, 2013
    Applicant: Ajinomoto Co., Inc.
    Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Enzyme that catalyzes a peptide-forming reaction from a carboxy component and an amine component, microbe producing the same, and a method of producing a dipeptide using the enzyme or microbe
    Patent number: 8329428
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: August 17, 2011
    Date of Patent: December 11, 2012
    Assignee: AJINOMOTO Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • NOVEL PEPTIDE-FORMING ENZYME GENE
    Publication number: 20120003693
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Application
    Filed: August 17, 2011
    Publication date: January 5, 2012
    Applicant: Ajinomoto Co., Inc.
    Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Method for producing a dipeptide
    Patent number: 8039232
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: October 18, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • NOVEL PEPTIDE-FORMING ENZYME GENE
    Publication number: 20100267082
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Application
    Filed: March 23, 2010
    Publication date: October 21, 2010
    Applicant: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Peptide-forming enzyme gene
    Patent number: 7736876
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: June 15, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Peptide-forming enzyme gene
    Patent number: 7736871
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: June 15, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method
    Patent number: 7618796
    Abstract: The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
    Type: Grant
    Filed: October 31, 2006
    Date of Patent: November 17, 2009
    Assignee: Ajinomoto Co., Inc.
    Inventors: Naoto Tonouchi, Sonoko Suzuki, Kenzo Yokozeki, Hiroyuki Nozaki, Masakazu Sugiyama
  • Peptide-forming enzyme gene
    Patent number: 7531340
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: May 12, 2009
    Assignee: AJINOMOTO Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • PEPTIDE-FORMING ENZYME GENE
    Publication number: 20080274530
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Application
    Filed: June 18, 2008
    Publication date: November 6, 2008
    Applicant: Ajinomoto Co., Inc.
    Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • NOVEL PEPTIDE-FORMING ENZYME GENE
    Publication number: 20080166761
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Application
    Filed: August 20, 2007
    Publication date: July 10, 2008
    Applicant: Ajinomoto Co., Inc.
    Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • NOVEL PEPTIDE-FORMING ENZYME GENE
    Publication number: 20080050773
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Application
    Filed: August 20, 2007
    Publication date: February 28, 2008
    Applicant: Ajinomoto Co., Inc.
    Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Peptide-forming enzyme gene
    Patent number: 7288389
    Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
    Type: Grant
    Filed: May 28, 2004
    Date of Patent: October 30, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
  • Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method
    Patent number: 7288388
    Abstract: The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
    Type: Grant
    Filed: January 26, 2004
    Date of Patent: October 30, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Naoto Tonouchi, Sonoko Suzuki, Kenzo Yokozeki, Hiroyuki Nozaki, Masakazu Sugiyama