Abstract: In some embodiments, the present disclosure provides methods for producing biofuel from a biological material that includes protein and a biofuel feedstock, such as triglycerides. In a specific example, the biological material is hydrolyzed to obtain the biofuel feedstock, such as by treatment with a base. Free fatty acids or triglycerides are then extracted using an organic solvent. The free fatty acids or triglycerides are converted to fatty acid esters, useable as biofuel, by esterification or transesterification, respectively. In a more specific example, the biological material is converted to a biofuel in a one step process by treating the biological material with base and an appropriate alcohol. In some implementations, a disclosed method uses chicken feathers obtained from a chicken processing operation as the biological material.