Abstract: Method and systems for performing ultrasound guided transperineal prostate biopsies. A template having a plurality of apertures and attached to a transrectal ultrasound probe is used for planning and guiding the biopsy. The template is placed against the patient's perineum and transverse ultrasound images having a projected template image are displayed. Planned biopsy locations are marked on the projected aperture image and biopsy samples are obtained through the corresponding template apertures. Sagittal ultrasounds may be viewed to ensure the correct depth of the biopsy needle during the biopsy. Information about the location of the biopsy sample can be recorded by identifying the template aperture through which the biopsy was obtained. Stored biopsy location information can be used for planning later treatments which can use a template having the same set of apertures.