Abstract: Methods for increasing targeted genome editing by down-regulating proteins involved in cytosolic DNA sensing pathways.

Abstract: The present disclosure provides a method for endogenously tagging an endogenous protein in a cell, and a cell comprising an endogenously tagged protein. Also described are cells produced using such a method and a kit comprising a cell having tagged endogenous protein.

Abstract: The present invention provides methods for using endogenous transcriptional control systems to regulate the expression of heterologous protein(s). In particular, targeted genome editing is used to integrate a sequence encoding the heterologous protein(s) in-frame with an endogenous coding sequence such that the expression of the heterologous and endogenous sequences is regulated by the endogenous control system.

Abstract: The present invention provides methods for using endogenous transcriptional control systems to regulate the expression of heterologous protein(s). In particular, targeted genome editing is used to integrate a sequence encoding the heterologous protein(s) in-frame with an endogenous coding sequence such that the expression of the heterologous and endogenous sequences is regulated by the endogenous control system.

Abstract: The present disclosure provides a method for endogenously tagging an endogenous protein in a cell, and a cell comprising an endogenously tagged protein. Also described are cells produced using such a method and a kit comprising a cell having tagged endogenous protein.

Abstract: The present invention provides compositions and methods for monitoring cellular signal transduction events. In particular, signal transduction events may be monitored by the use of biosensors comprising protein-binding domains, which bind signal-transduction proteins, and fluorescent proteins. Provided herein, therefore, methods for detecting activation of signal transduction proteins or screening for agents that modulate the activity of signal transduction proteins. Also provided are cells comprising the biosensors; lentiviral particles comprising biosensor coding sequence; and kits comprising the lentiviral particles.

Abstract: The present invention generally relates to polypeptide tags and the purification of polypeptides, proteins, and protein fragments displaying such tags. More particularly, the invention is directed to novel polypeptides, nucleic acid sequences and vectors encoding novel polypeptides containing antigenic determinants. Also provided are methods and kits for using such polypeptides for the purification of target peptides, as well as methods of constructing vectors encoding the novel polypeptides and the novel polypeptides linked to a target peptide.

Abstract: This invention provides methods, compounds, and kits for identifying Echinacea species and for distinguishing between Echinacea and imposter species based upon molecular variations. In particular, amplification primers are provided that amplify specific regions of nucleic acid sequences to generate unique amplification profiles in the different plant species. A plant may be identified by comparing its amplification profile to known amplification profiles of Echinacea and imposter species.

Abstract: This invention provides methods, compounds, and kits for identifying ginseng (Panax) species and for distinguishing between ginseng and imposter species based upon molecular variations. In particular, amplification primers are provided that amplify polymorphic regions of nucleic acid sequences to generate distinct amplification profiles in the different plant species. A plant may be identified by comparing its amplification profile to known amplification profiles of Panax and imposter species.