Abstract: It is provided a simple and efficient process for producing a large amount of protein in a cell-free protein synthesis system, through a continuous synthesis reaction over a long time using a linear DNA as a template. The continuous synthesis reaction is carried out for at least one hour with a cell-free protein synthesis system comprising an extract of Escherichia coli cells cultured at any temperature between 18° C. and 36° C., and a linear template DNA encoding the protein. The continuous protein synthesis system is preferably a dialysis system or a continuous flow system. The cell-free protein synthesis system by dialysis system comprises an internal dialysate comprising the extract and the linear template DNA, an external dialysate comprising a substrate solution for protein synthesis, and a dialysis membrane through which the substrate can be transferred.

Abstract: It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.

Abstract: It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.

Abstract: It is provided a simple and efficient process for producing a large amount of protein in a cell-free protein synthesis system, through a continuous synthesis reaction over a long time using a linear DNA as a template. The continuous synthesis reaction is carried out for at least one hour with a cell-free protein synthesis system comprising an extract of Escherichia coli cells cultured at any temperature between 18° C. and 36° C., and a linear template DNA encoding the protein. The continuous protein synthesis system is preferably a dialysis system or a continuous flow system. The cell-free protein synthesis system by dialysis system comprises an internal dialysate comprising the extract and the linear template DNA, an external dialysate comprising a substrate solution for protein synthesis, and a dialysis membrane through which the substrate can be transferred.

Abstract: It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.

Abstract: A moving picture processing apparatus comprises an image capture unit for taking in time-series images (moving picture series) of a target object, a feature point extraction unit for extracting feature points constituting the surface of the object whose model is to be made, a three-dimensional position information extraction unit for finding three-dimensional position information on the feature points by correlating the feature points contained in the image at a point in time with those at another point in time in the time-series images and analyzing positional coordinate information on the correlated feature points, a three-dimensional patch group generating unit for generating three-dimensional patch groups constituting the surface of the target object on the basis of the feature points for which the three-dimensional position information has been found and supplying the three-dimensional patch groups as information on a model of the target object to an output section.